MCP Thermo Scientific TMT Isobaric Mass Tagging Kits
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on February 1, 2005.
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M400076-MCP200v1
M400076-MCP200v2
4/2/120    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jimenez, C. R.
Right arrow Articles by Smit, A. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jimenez, C. R.
Right arrow Articles by Smit, A. B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on June 28, 2004
Revised on October 19, 2004
Accepted on October 25, 2004

Proteomics of the injured rat sciatic nerve reveals protein expression dynamics during regeneration

Connie R. Jimenez, Floor J. Stam, Ka Wan Li, Yvonne Gouwenberg, Martin P. Hornshaw, Fred De Winter, Joost Verhaagen, and August B. Smit

Molecular&Cellular Neurobiology, Vrije Universiteit, Amsterdam 1081 HV

Corresponding Author: connie.jimenez{at}falw.vu.nl

Using proteomics, we investigated the temporal expression profiles of proteins in rat sciatic nerve after experimental crush. Extracts of sciatic nerves collected at 5, 10 and 35 days after injury were analyzed by two-dimensional gel electrophoresis and quantitative image analysis. Of the ~1500 protein spots resolved on each gel, 121 showed significant regulation during at least one time point. Using cluster analysis these proteins were grouped into two expression profiles of down-regulation and four of up regulation. These profiles mainly reflected differences in cellular origins in addition to different functional roles. Mass spectrometric analysis identified 82 proteins pertaining to several functional classes, i.e., acute phase proteins, antioxidant proteins and proteins involved in protein synthesis/ maturation/ degradation, cytoskeletal (re)organization and in lipid metabolism. Several proteins not previously implicated in nerve regeneration were identified, e.g., translationally-controlled tumor protein, annexin A9/31, vitamin D-binding protein, a-crystallin B, a-synuclein, dimethylargininases and reticulocalbin. Real-time PCR analysis of selected genes showed which were expressed in the nerve vs the dorsal root ganglion neurons. In conclusion, this study highlights the complexity and temporal aspect of the molecular process underlying nerve regeneration and points to the importance of glial and inflammatory determinants.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
M.-C. Tsai, L.-F. Shen, H.-S. Kuo, H. Cheng, and K.-F. Chak
Involvement of Acidic Fibroblast Growth Factor in Spinal Cord Injury Repair Processes Revealed by a Proteomics Approach
Mol. Cell. Proteomics, September 1, 2008; 7(9): 1668 - 1687.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
E. K. Bichler, S. T. Nakanishi, Q.-B. Wang, M. J. Pinter, M. M. Rich, and T. C. Cope
Enhanced Transmission at a Spinal Synapse Triggered In Vivo by an Injury Signal Independent of Altered Synaptic Activity
J. Neurosci., November 21, 2007; 27(47): 12851 - 12859.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
N. Komori, N. Takemori, H. K. Kim, A. Singh, S.-H. Hwang, R. D. Foreman, K. Chung, J. M. Chung, and H. Matsumoto
Proteomics study of neuropathic and nonneuropathic dorsal root ganglia: altered protein regulation following segmental spinal nerve ligation injury
Physiol Genomics, April 24, 2007; 29(2): 215 - 230.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.