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Submitted on November 11, 2004
Center for Research on Reproduction & Women's Health, University of Pennsylvania, Philadelphia, PA 19104
Corresponding Author: jfs3{at}mail.med.upenn.edu
The axoneme central apparatus is thought to control flagellar/ciliary waveform and maintain the structural integrity of the axoneme, but proteins involved in these processes have not been fully elucidated. Moreover, the network of interactions among them which allow these events to take place in a compact space have not been defined. PF6, a component of the Chlamydomonas central apparatus, is localized to the 1a projection of the C1 microtubule. Mutations in the Chlamydomonas PF6 gene result in flagellar paralysis. We characterized human and murine orthologues of PF6. The murine Pf6 gene is expressed in a pattern consistent with a role in flagella and cilia, and that PF6 protein is indeed localized to the central apparatus of the sperm flagellar axoneme. We discovered that a portion of PF6 associates with the mammalian orthologue of Chlamydomonas PF16 (SPAG6), another central apparatus protein that is localized to the C1 microtubule in algae. A fragment of PF6 corresponding to the PF6 domain that interacts with SPAG6 in yeast 2-hybrid assays and colocalizes with SPAG6 in transfected cells, was missing from epididymal sperm of SPAG6-deficient mice. SPAG6 binds to the mammalian orthologue of PF20, which in Chlamydomonas is located in bridges connecting the C2 and C1 microtubules. Thus, PF6, SPAG6 and PF20 form a newly identified network that links together components of the axoneme central apparatus, and presumably participate in its dynamic regulation of ciliary and flagellar beat.
Revised on April 4, 2005
Accepted on April 12, 2005
Dissecting the axoneme interactome: The mammalian orthologue of chlamydomonas PF6 interacts with SPAG6, the mammalian orthologue of chlamydomonas PF16
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