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Submitted on December 7, 2004
Revised on March 5, 2005
Accepted on March 5, 2005

Proteomic analysis of primary cultures of human adipose derived stem cells: Modulation by adipogenesis

James Delany, Z. Elizabeth Floyd, Sanjin Zvonic, Andrea Smith, Amy Gravois, Eric Reiners, Xiying Wu, Gail Kilroy, Michael Lefevre, and Jeffrey M. Gimble

Stem Cell Laboratory, Pennington Biomedical Research Center, Baton Rouge, LA 70808

Corresponding Author: gimblejm{at}pbrc.edu

Adipogenesis plays a critical role in energy metabolism and is a contributing factor to the obesity epidemic. This study examined the proteome of primary cultures of human adipose-derived adult stem (ADAS) cells as an in vitro model of adipogenesis. Protein lysates obtained from four individual donors were compared before and after adipocyte differentiation by 2-dimensional gel electrophoresis and tandem mass spectroscopy. Over 170 individual protein features in the undifferentiated ADAS cells were identified. Following adipogenesis, over 40 proteins were up-regulated by =2-fold while 13 showed a =3-fold reduction. The majority of the modulated proteins belonged to the following functional categories: cytoskeleton, metabolic, redox, protein degradation, and heat shock protein/chaperones. Additional immunoblot analysis documented the induction of four individual heat shock proteins and confirmed the presence of the heat shock protein 27 phosphoserine 82 isoform, as predicted by the proteomic analysis, as well as the crystallin a phosphorylated isoforms. These findings suggest that the heat shock protein family proteome warrants further investigation with respect to the etiology of obesity and type 2 diabetes.


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