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Submitted on June 6, 2005
Revised on July 21, 2005
Accepted on July 25, 2005

Analysis of leaf proteome after UV-B irradiation in maize lines differing in sensitivity

Paula Casati, Xing Zhang, Alma L. Burlingame, and Virginia Walbot

Department of Biological Sciences, Universidad Nacional de Rosario, Rosario, Santa Fe 2000

Corresponding Author: paulacasati{at}gmail.com

UV-B radiation causes diverse morphological and physiological responses in plants, but the underlying mechanisms governing these integrated responses are unknown. In this study, we systematically surveyed responses of maize leaves to UV-B radiation using DIGE 2D gels and identified selected proteins by mass spectrometry and immunodetection analysis. To identify changes in protein accumulation in response to UV-B radiation, a line (b, pl W23) deficient in flavonoid sunscreen compounds and hence similar to commercial corn was used. In addition, its proteome in natural UV-B conditions was compared to that of two maize landraces from high altitudes (Cacahuacintle, and Confite Puneño) that have improved UV-B tolerance. Protein patterns in adult maize leaves (Zea mays) were documented after growth for 21 days in sunlight depleted of UV-B or growth in sunlight including an 8h UV-B supplementation during one day in the field. We found that there is a very high correlation between previously documented mRNA accumulation assessed by microarray hybridization and qRT-PCR and protein expression after UV-B irradiation in leaves of W23. Multiple isoforms were confirmed for some proteins; at least one protein, pyruvate phosphate dikinase, is regulated post-translationally by phosphorylation by UV-B exposure. Proteins differentially regulated by UV-B in W23 with higher levels under similar UV-B conditions in high altitude plants were also identified. These could be genetically fixed traits conferring UV-B tolerance and offer clues to specific adaptations to living in high ambient UV-B conditions.


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