MCP AbD Serotec
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on December 1, 2005.
This Article
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M500207-MCP200v1
4/12/2000    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hu, Y.
Right arrow Articles by Holtzman, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hu, Y.
Right arrow Articles by Holtzman, D. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Submitted on July 11, 2005
Revised on September 30, 2005
Accepted on September 30, 2005

Comparative proteomics analysis of intra- and inter-individual variation in human cerebrospinal fluid

Yan Hu, James P. Malone, Anne M. Fagan, R. Reid Townsend, and David M. Holtzman

Neurology Dept., Washington University, St. Louis, MO 63110

Corresponding Author: holtzman{at}neuro.wustl.edu

Cerebrospinal fluid (CSF) is a potential source of biomarkers for many disorders of the central nervous system (CNS), including Alzheimer’s disease (AD). Prior to comparing CSF samples between individuals to identify patterns of disease-associated proteins, it is important to examine variation within individuals over a short period of time so that one can better interpret potential changes in CSF between individuals, as well as changes within a given individual over a longer time span. In this study, we analyzed 12 CSF samples, composed of pairs of samples from 6 individuals, obtained 2 weeks apart. Multi-affinity depletion, 2-D DIGE and tandem mass spectrometry were employed. A number of proteins whose abundance varied between the two time points were identified for each individual. Some of these proteins were commonly identified in multiple individuals. More importantly, despite the intra-individual variations, hierarchical clustering and multidimensional-scaling analysis of the proteomic profiles revealed that two CSF samples from the same individual cluster the closest together, and the between-subject variability is much larger than the within-subject variability. Among the 6 subjects, comparison between the 4 cognitively normal and the 2 very mildly demented subjects also yielded some proteins that have been identified in previous AD biomarker studies. These results validate our method of identifying differences in proteomic profiles of CSF samples and have important implications for the design of CSF biomarker studies for AD and other CNS disorders.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J AndrolHome page
K. Yamakawa, K. Yoshida, H. Nishikawa, T. Kato, and T. Iwamoto
Comparative Analysis of Interindividual Variations in the Seminal Plasma Proteome of Fertile Men With Identification of Potential Markers for Azoospermia in Infertile Patients
J Androl, November 1, 2007; 28(6): 858 - 865.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
T. Liu, W.-J. Qian, M. A. Gritsenko, W. Xiao, L. L. Moldawer, A. Kaushal, M. E. Monroe, S. M. Varnum, R. J. Moore, S. O. Purvine, et al.
High Dynamic Range Characterization of the Trauma Patient Plasma Proteome
Mol. Cell. Proteomics, October 1, 2006; 5(10): 1899 - 1913.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
W.-J. Qian, J. M. Jacobs, T. Liu, D. G. Camp II, and R. D. Smith
Advances and Challenges in Liquid Chromatography-Mass Spectrometry-based Proteomics Profiling for Clinical Applications
Mol. Cell. Proteomics, October 1, 2006; 5(10): 1727 - 1744.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.