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Submitted on August 25, 2005
Accepted on August 26, 2005

A human protein atlas for normal and cancer tissues based on antibody proteomics

Mathias Uhlen, Erik Bjorling, Charlotta Agaton, Cristina Al-Khalili Szigyarto, Bahram Amini, Elisabet Andersen, Ann-Catrin Andersson, Pia Angelidou, Anna Asplund, Caroline Asplund, Lisa Berglund, Kristina Bergstrom, Harry Brumer, Dijana Cerjan, Marica Ekstrom, Adila Elobeid, Cecilia Eriksson, Linn Fagerberg, Ronny Falk, Jenny Fall, Mattias Forsberg, Marcus Gry Bjorklund, Kristoffer Gumbel, Asif Halimi, Inga Halllin, Carl Hamsten, Marianne Hansson, My Hedhammar, Gorel Hercules, and Caroline Kampf

Royal Institute of Technology (KTH), Stockholm 10691

Corresponding Author: mathias{at}biotech.kth.se

Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We have used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here, we report of a new publicly available database containing, in the first version, approximately 400,000 high-resolution images corresponding to more than 700 antibodies towards human proteins (www.proteinatlas.org). Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.


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