| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on September 28, 2005
Department of Anesthesia and Perioperative Care, University of California San Francisco, San Francisco, CA 94143-0648
Corresponding Author: hirschj{at}anesthesia.ucsf.edu
The aim of the study was to investigate the impact of ischemia reperfusion injury on the proteome of Kupffer cells. Lean Zucker rats (n=6 each group) were randomized to 75 min of warm ischemia or sham operation. After reperfusion for 8h, Kupffer cells were isolated by enzymatic perfusion and density gradient centrifugation. Proteins were tryptically digested into peptides, and differentially labeled with iTRAQ reagent. After fractionation by cation exchange chromatography, peptides were identified by mass spectrometry (ESI-LC-MS-MS). Spectra were interrogated against the Swissprot database and quantified using ProteinProspector®. The results for heat shock protein 70 and myeloperoxidase were validated by ELISA. Quantitative information for more than 1559 proteins was obtained. In the ischemia group proteins involved in inflammation were significantly up-regulated. The ratio for calgranulin b in the ischemia/sham group was 1.81±0.97 (p<0.0001) for complement C3 1.81±0.49 (p<0.0001) and myeloperoxidase (1.30±0.32). Myeloperoxidase was only recently documented in Kupffer cells. The antioxidative proteins superoxide dismutase [CuZn] (1.34±0.19; p<0.001) and catalase (1.23±0.43; p<0.001) were also elevated. In conclusion, ischemia reperfusion injury induces alterations in the Kupffer cell proteome. Isotope ratio mass spectrometry is a powerful tool to investigate these reactions. The ability to simultaneously monitor several pathways involved in reperfusion stress may result in important mechanistic insight and possibly new treatment options
Revised on February 8, 2006
Accepted on February 23, 2006
Warm ischemia induced alterations in oxidative and inflammatory proteins in hepatic Kupffer cells in rats
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Pisitkun, J. D. Hoffert, M.-J. Yu, and M. A. Knepper Tandem Mass Spectrometry in Physiology Physiology, December 1, 2007; 22(6): 390 - 400. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. B. Nicoud, C. D. Knox, C. M. Jones, C. D. Anderson, J. M. Pierce, A. E. Belous, T. M. Earl, and R. S. Chari 2-APB protects against liver ischemia-reperfusion injury by reducing cellular and mitochondrial calcium uptake Am J Physiol Gastrointest Liver Physiol, September 1, 2007; 293(3): G623 - G630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Hirsch, K. C. Hansen, A. Sapru, J. A. Frank, R. J. Chalkley, X. Fang, J. C. Trinidad, P. Baker, A. L. Burlingame, and M. A. Matthay Impact of Low and High Tidal Volumes on the Rat Alveolar Epithelial Type II Cell Proteome Am. J. Respir. Crit. Care Med., May 15, 2007; 175(10): 1006 - 1013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. B. Nicoud, C. M. Jones, J. M. Pierce, T. M. Earl, L. M. Matrisian, R. S. Chari, and D. L. Gorden Warm Hepatic Ischemia-Reperfusion Promotes Growth of Colorectal Carcinoma Micrometastases in Mouse Liver via Matrix Metalloproteinase-9 Induction Cancer Res., March 15, 2007; 67(6): 2720 - 2728. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Journal of Lipid Research | ASBMB Today |
