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A more recent version of this article appeared on June 1, 2006. Originally published In Press as doi:10.1074/mcp.M500320-MCP200 on February 24, 2006.
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Submitted on September 28, 2005
Revised on February 8, 2006
Accepted on February 23, 2006

Warm ischemia induced alterations in oxidative and inflammatory proteins in hepatic Kupffer cells in rats

Jan Hirsch, Kirk C. Hansen, SooJinNa Choi, Joonhwa Noh, Ryutaro Hirose, John P. Roberts, Michael A. Matthay, Alma L. Burlingame, Jacquelyn J. Maher, and Claus U. Niemann

Department of Anesthesia and Perioperative Care, University of California San Francisco, San Francisco, CA 94143-0648

Corresponding Author: hirschj{at}anesthesia.ucsf.edu

The aim of the study was to investigate the impact of ischemia reperfusion injury on the proteome of Kupffer cells. Lean Zucker rats (n=6 each group) were randomized to 75 min of warm ischemia or sham operation. After reperfusion for 8h, Kupffer cells were isolated by enzymatic perfusion and density gradient centrifugation. Proteins were tryptically digested into peptides, and differentially labeled with iTRAQ reagent. After fractionation by cation exchange chromatography, peptides were identified by mass spectrometry (ESI-LC-MS-MS). Spectra were interrogated against the Swissprot database and quantified using ProteinProspector®. The results for heat shock protein 70 and myeloperoxidase were validated by ELISA. Quantitative information for more than 1559 proteins was obtained. In the ischemia group proteins involved in inflammation were significantly up-regulated. The ratio for calgranulin b in the ischemia/sham group was 1.81±0.97 (p<0.0001) for complement C3 1.81±0.49 (p<0.0001) and myeloperoxidase (1.30±0.32). Myeloperoxidase was only recently documented in Kupffer cells. The antioxidative proteins superoxide dismutase [CuZn] (1.34±0.19; p<0.001) and catalase (1.23±0.43; p<0.001) were also elevated. In conclusion, ischemia reperfusion injury induces alterations in the Kupffer cell proteome. Isotope ratio mass spectrometry is a powerful tool to investigate these reactions. The ability to simultaneously monitor several pathways involved in reperfusion stress may result in important mechanistic insight and possibly new treatment options


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