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Submitted on December 5, 2005
Revised on February 27, 2006
Accepted on March 8, 2006

Molecular dynamics of the Shewanella oneidensis response to chromate stress

Steven D. Brown, Melissa R. Thompson, Nathan C. VerBerkmoes, Karuna Chourey, Manesh Shah, Jizhong Zhou, Robert L. Hettich, and Dorothea K. Thompson

Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6131

Corresponding Author: hettichrl{at}ornl.gov

Temporal genomic profiling and whole-cell proteomic analyses were performed to characterize the dynamic molecular response of the metal-reducing bacterium Shewanella oneidensis MR-1 to an acute chromate shock. The complex dynamics of cellular processes demand the integration of methodologies that describe biological systems at the levels of regulation, gene and protein expression, as well as metabolite production. Genomic microarray analysis of the transcriptome dynamics of mid-exponential-phase cells subjected to 1 mM potassium chromate (K2CrO4) at exposure time intervals of 5, 30, 60 and 90 min revealed 910 genes that were differentially expressed at one or more time points. Differential proteomics based on multidimensional high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to complement the transcriptome data, resulting in comparable induction and repression patterns for a subset of corresponding proteins. The initial response of S. oneidensis to chromate shock appears to require a combination of different regulatory networks that involve genes with annotated functions in oxidative stress protection, detoxification, protein stress protection, iron and sulfur acquisition, and SOS-controlled DNA repair mechanisms.


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