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Submitted on February 3, 2006
UMR 692 Phytopharmacie et Biochimie des Interactions Cellulaires, INRA, Dijon, Cedex 21065
Corresponding Author: simon{at}epoisses.inra.fr
A large body of evidence from the past decade supports the existence, in membrane from animal and yeast cells, of functional microdomains which play important roles in protein sorting, signal transduction, or infection by pathogens. Recent reports demonstrated the presence, in plants, of detergent resistant fractions isolated from plasma membrane. Analysis of the lipidic composition of this fraction revealed its enrichment in sphingolipid and sterols, and depletion in phospho and glycerolipids, as previously observed for animal microdomains. One- dimensional gel electrophoresis experiments indicated that these detergent resistant fractions were able to recruit a specific set of plasma membrane proteins and exclude others. In the present study, we used mass spectrometry to give an extensive description of a tobacco plasma membrane fraction resistant to solubilization with Triton X-100. This led to the identification of 145 proteins whose functional and physico-chemical characteristics were analysed in silico. Parameters such as isoelectric point, molecular weight, number and length of transmembrane segments, or global hydrophobicity were analysed and compared to the data available concerning plant plasma membrane proteins. Post-traductional modifications such as myristoylation, palmitoylation or presence of a GPI-anchor, have been examined in relation to the presence of the corresponding proteins in these microdomains. From a functional point of view, this analysis indicated that if a primary function of the plasma membrane, such as transport, seems underrepresented in the detergent resistant fraction, others undergo a significant increase of their relative importance. Among these are : signalling and response to biotic and abiotic stress, cellular trafficking and cell wall metabolism. This suggests that these domains are likely to consitute, as in animal cells, signalling platforms involved in these physiological functions.
Revised on March 31, 2006
Accepted on April 28, 2006
Proteomics of plant detergent resistant membranes
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