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Submitted on March 6, 2006
Accepted on March 8, 2006

From gene expression analysis to tissue microarrays – a rational approach to identify therapeutic and diagnostic targets in lymphoid malignancies

Sara Ek, Ulrika Andréasson, Sophia Hober, Caroline Kampf, Fredrik Pontén, Mathias Uhlén, Hartmut Merz, and Carl A. K. Borrebaeck

Department of Immunotechnology, Lund University, LUND 220 07

Corresponding Author: sara.ek{at}immun.lth.se

Mantle cell lymphoma (MCL) is an aggressive lymphoid malignancy for which better treatment strategies are needed. To identify potential diagnostic and therapeutic targets, a signature consisting of MCL-associated genes was selected, based on a comprehensive gene expression analysis of malignant and normal B cells. The corresponding protein epitope signature tags (PrESTs) were identified and used to raise mono-specific, polyclonal antibodies, which were subsequently analyzed on paraffin-embedded sections of malignant and normal tissue. In this study, we demonstrate that the initial selection strategy of MCL-associated genes successfully allows identification of protein antigens either uniquely expressed or overexpressed in MCL, compared to normal lymphoid tissues. We propose that genome-based, affinity proteomics, using PrEST-induced antibodies, is an efficient way to rapidly identify a number of disease-associated protein candidates of previously both known and unknown identity.


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