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Submitted on April 19, 2006
Revised on October 10, 2006
Accepted on November 27, 2006

Proteomic identification of differentially expressed proteins associated with pollen germination and tube growth reveals characteristics of germinated Oryza sativa pollen

Shao Jun Dai, Tao Tao Chen, Kang Chong, Yong Biao Xue, Si Qi Liu, and Tai Wang

Research Center for Molecular & Developmental Biology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093

Corresponding Author: twang{at}ibcas.ac.cn

Mature pollen from most plant species is metabolically quiescent; however, after pollination, it germinates quickly and gives rise to a pollen tube to transport sperm into the embryo sac. Because methods for collecting a large amount of in vitro-germinated pollen grains for transcriptomic and proteomic studies from model plants of Arabidopsis and rice are not available, molecular information about the germination developmental process is lacking. Here, we describe a method for obtaining a large quantity of in vitro-germinating rice pollen for proteomic study. 2-D electrophoresis of approximately 2300 protein spots revealed 186 that were differentially expressed in mature and germinated pollen. Most showed a changed level of expression, and only 66 appeared to be specific to developmental stages. Furthermore, 160 differentially expressed protein spots were identified on mass spectrometry to match 120 diverse protein species. These proteins involve different cellular and metabolic processes with obvious functional skew onto wall metabolism, protein synthesis and degradation, cytoskeleton dynamics and carbohydrate/energy metabolism. Wall metabolism-related proteins are prominently featured in the differentially expressed proteins and the pollen proteome as compared with in rice sporophytic proteomes. Our study also reveals multiple isoforms and differential expression patterns between isoforms of a protein. These results provide novel insights into pollen function specialization.


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