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Submitted on June 27, 2006
Accepted on July 6, 2006

A quantitative results-driven approach to analyzing multi-site protein phosphorylation: The phosphate-dependent phosphorylation profileof the transcription factor pho4

Francesca Zappacosta, Therese S. Collingwood, Michael J. Huddleston, and Roland S. Annan

Computational, Analytical and Structural Sciences, GlaxoSmithKline Pharm, King of Prussia, PA 19406

Corresponding Author: roland_s_annan{at}gsk.com

This paper describes the using of N-terminal isotope labeling and precursor ion scanning for the quantitative analysis of protein phosphorylation. The regulation of the budding yeast transcription factor Pho4 via nutrient sensitive phosphorylation is studied using this technique.


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