Submitted on March 15, 2007
Revised on September 17, 2007
Accepted on October 13, 2007
Protein expression profiling of breast cancer cells by dissociable antibody microarray (DAMA) staining
X. Cynthia Song, Guanyuan Fu, Xufen Yang, Zhong Jiang, Yingjian Wang, and G. Wayne Zhou
Biological Sciences, Louisiana State University, Baton Rouge, LA 70803
Corresponding Author: zhouw{at}lsu.edu
Dissociable Antibody MicroArray (DAMA) staining is a technology that combines protein microarrays with traditional immunostaining techniques. It can simultaneously determine the expression and subcellular location of hundreds of proteins in cultured cells and tissue samples. We have developed this technology and demonstrated its application in identifying potential biomarkers for breast cancer. We have compared the expression profiles of 312 proteins among three normal breast cell lines and seven breast cancer cell lines, and have identified ten differentially expressed proteins by the data analysis program DAMAPEP. Among those proteins, RAIDD, Rb p107, Rb p130, SRF and Tyk2 have been confirmed by Western blot and statistical analysis to have higher expression levels in breast cancer cells than in normal breast cells. These proteins could be potential biomarkers for the diagnosis of breast cancer.
