Serum Microarrays for Large Scale Screening of Protein Levels *S

  1. Peter Nilsson**
  1. From the ‡Division of Clinical Immunology, Karolinska Institute, Karolinska Hospital in Huddinge, SE-14186 Stockholm, Sweden and Department of Molecular Biotechnology, KTH-Royal Institute of Technology, AlbaNova University Center, SE-10691, Stockholm, Sweden
  1. ‖To whom correspondence may be addressed. Tel.: 46-8-5248-3586; Fax: 46-8-5248-3588; E-mail: lennart.hammarstrom{at}labmed.ki.se

Abstract

There is a great need for comprehensive proteomic analysis of large patient cohorts of plasma and serum samples to identify biomarkers of human diseases. Here we describe a new antibody-based proteomic approach involving a reverse array format where serum samples are spotted on a microarray. This enables all samples to be screened for their content of a certain serum protein in a single experiment using target-recognizing antibodies and fluorescently labeled secondary antibodies. The procedure is illustrated with the analysis of the IgA levels in 2009 spotted serum samples, and the data are compared with clinical routine measurements. The results suggest that it is possible to simultaneously screen thousands of complex clinical serum samples for their content of the relative amount of specific serum proteins of clinical relevance.

Footnotes

  • Published, MCP Papers in Press, August 29, 2005, DOI 10.1074/mcp.M500213-MCP200

  • ↵1 The abbreviations used are: RPP, reverse phase protein; IgAD, IgA deficiency; CV, coefficient of variation; C3, third component of complement.

  • ↵* This research was supported by the Swedish Research Council, European Union (MolPAGE), and Knut and Alice Wallenberg Foundation

  • ↵S The on-line version of this article (available at http://www.mcponline.org) contains supplemental material.

  • ↵§ Both authors contributed equally to this work.

  • ↵** To whom correspondence may be addressed. Tel.: 46-8-5537-8331; Fax: 46-8-5537-8481; E-mail: peter.nilsson{at}biotech.kth.se

    • Received July 14, 2005.
    • Revision received August 18, 2005.
View this article with LENS
| Table of Contents

This Article

  1. First Published on August 29, 2005, doi: 10.1074/mcp.M500213-MCP200 Molecular & Cellular Proteomics, 4, 1942-1947.
  1. » AbstractFree
  2. Full TextFree
  3. Full Text (PDF)Free
  4. Supplemental Data
  5. All Versions of this Article:
    1. M500213-MCP200v1
    2. 4/12/1942 most recent

Classifications

Article Usage Stats

  1. Article Usage Statistics

Responses

  1. Submit a Letter to the Editor
  2. No responses published

Current Issue

  1. July 2017, 16 (7)
  1. Current Issue
  1. Alert me to new issues of Molecular & Cellular Proteomics