Table IV

A proteome-wide prediction of Aurora-B-specific substrate sites

The predicted phosphorylation sites are bold. ROCK, Rho kinase; PKC, protein kinase C; LOK, lymphocyte-oriented kinase; PAK, p21-activated kinase; GFAP, glial fibrillary acidic protein; DES, desmin; NES, nestin; VIM, vimentin; AURKA, Aurora kinase A; CENPA, centromere protein A; BPTF, bromodomain PHD finger transcription factor; MCAK, mitotic centromere-associated kinesin; INCENP, inner centromere protein.

SubstratePhospho.ELMSitePeptideGPS scoreKnown kinases
HEC1O147775 ---MKRSSVSSGGAG 6.1515Aurora-B
O1477715 SGGAGRLSMQELRSQ 4.8788Aurora-B
O1477749 KLSINKPTSERKVSL 3.8182Aurora-B
O1477755 PTSERKVSLFGKRTS 5.3636Aurora-B
O1477762 SLFGKRTSGHGSRNS 3.2424
O1477769 SGHGSRNSQLGIFSS 4.303Aurora-B
AURKAO14965288 APSSRRTTLCGTLDY 5.8485PKA, Aurora-A
PPP1R12AO14974696 ARQSRRSTQGVTLTD 5.0606ROCK1
SurvivinO15392117 KNKIAKETNNKKKEF 4.2424Aurora-B
VIMP0867065 GVYATRSSAVRLRSS 4.303PAK
P0867072 SAVRLRSSVPGVRLL 4.5758PAK, ROCK, Aurora-B
GFAPP141367 -MERRRITSAARRSY 8.303ROCK, Aurora-B
P1413613 ITSAARRSYVSSGEM 7.5152ROCK, PKC, CaMKII, Aurora-B
P1413638 LGPGTRLSLARMPPP 4.5455ROCK, PKC, CaMKII, Aurora-B
STMN1P1694962 AAEERRKSHEAEVLK 5.8182PKA
DESP1766111 YSSSQRVSSYRRTFG 4.7576Aurora-B
P1766116 RVSSYRRTFGGAPGF 5.2727ROCK, Aurora-B
P1766159 VYQVSRTSGGAGGLG 4.8788Aurora-B
LMNB1P2070027 PLSPTRLSRLQEKEE 3.303
PSMA3P25788242 AEKYAKESLKEEDES 3.1818CK2
CDC25BP30305353 VQNKRRRSVTPPEEQ 6Aurora-A
BDKRB2P30411373 SMGTLRTSISVERQI 3.6364GRK-4, PKC
NESP48681767 ETQQRRRSLGEQDQM 6.8788
CENPAP494507 -MGPRRRSRKPEAPR 6.3636Aurora-A, Aurora-B
PLK1P53350137 LELCRRRSLLELHKR 3.2424
P53350210 YDGERKKTLCGTPNY 3.2121LOK
H3.1P6843110 TKQTARKSTGGKAPR 10.1212Aurora-A, Aurora-B
P6843128 ATKAARKSAPATGGV 8.9091MAPK, Aurora-B
MDM2Q00987157 SHLVSRPSTSSRRRA 4.0909
KIF23Q02241911 NGSRKRRSSTVAPAQ 6.303
Q02241912 GSRKRRSSTVAPAQP 6.3939
RELAQ04206276 SMQLRRPSDRELSEP 3.2121RSK-5
BPTFQ1283077 PRVHRPRSPILEEKD 3.0303
TP53BP1Q128881460 GAGALRRSDSPEIPF 3.2424
CBX3Q1318593 KDGTKRKSLSDSESD 5.1212
PIN1Q1352616 PGWEKRMSRSSGRVY 3.1818PKA
IFI16Q16666132 GAQKRKKSTKEKAGP 4.8485CK2
RCC1Q6NT9711 KRIAKRRSPPADAIP 4.4545
FLJ37981Q8N1Q373 ETSSLRNSQSENSSL 5
MCAKQ9966195 IQKQKRRSVNSKIPA 7.5455Aurora-B
RACGAP1Q9H0H5387 ETGLYRISGCDRTVK 3.7879Aurora-B
INCENPQ9NQS7897 KPRYHKRTSSAVWNS 4.1515Aurora-B
Q9NQS7898 PRYHKRTSSAVWNSP 5.7273Aurora-B
Q9NQS7899 RYHKRTSSAVWNSPP 3.1818Aurora-B
TD-60Q9P25843 RERPERCSSSSGGGS 4.1515
BAZ1BQ9UIG0189 EDEGRRESINDRARR 5.7576
Q9UIG01342 KRSSRRQSLELQKCE 4.6061
CDC23Q9UJX2582 NTPTRRVSPLNLSSV 3.7879