Table I

Antibodies used in this study. mAb1 is a monoclonal antibody raised against a peptide. mAb2 through mAb5 are monoclonal antibodies with epitopes previously determined in the literature. All of the polyclonal antibodies were raised against peptides selected from the random sequence peptide array

NameImmunogenClonalityIsotypeClone nameSequencepIHydropathicity
mAb1HA peptideMonoclonalIgG116B12YPYDVPDYA3.56−0.9
mAb2Human IL2MonoclonalIGg1LNKB2KPLEEVLNL4.53−0.044
mAb3Human p53MonoclonalIgG1PAb240RHSVV9.76−0.02
mAb4Human p53MonoclonalIgG2b, IgG2aDO-7, BP53–12SDLWKL5.55−0.25
mAb5Human Tubulin-alphaMonoclonalIgG1kappaDM1AAALEKD4.67−0.583
pAb1KLH-peptidePolyclonalIgG detectedNAMDQDDGEGVIGHFHPILGSC4.21−0.185
pAb2KLH-peptidePolyclonalIgG detectedNAEFWDKEWHTRADWPVWDGSC4.43−1.245
pAb3KLH-peptidePolyclonalIgG detectedNATIPAHNIFWILYFSIGTGSC6.40.87
pAb4KLH-peptidePolyclonalIgG detectedNAPAMKHREPHWVIPGIIWGSC8.64−0.13
pAb5KLH-peptidePolyclonalIgG detectedNAEFSNPTAQVFPDFWMSDGSC3.49−0.315