Table III

Proteins identified by MS/MS that are “known” or “likely” axonemal components

DescriptionAccession No.2-D gel1-D gelDirect LC/MS/MSMulti-dimensional LC/MS/MS
1α-TubulinMultipleXXXX
2β-TubulinMultipleXXXX
370-kDa heat shock protein(s)MultipleXXXX
4Heat shock protein(s) J2MultipleXXXX
5ActinMultipleXXXX
6Stratagene fetal retinalAA176890X
7AKAP-associated sperm proteinAAG59587XX
8Testicular microtubules-related protein Tektin 3AAK15340X
9Mus Musculus male testis cDNAAK005609X
10Mus Musculus male testis cDNAAK005739X
11Mus Musculus male testis cDNAAK015697X
12H-Tektin-TMultipleXX
13Human nucleoside diphosphate kinase homologP56597XXXX
14c-Myc-binding proteinQ99417XX
15Annexin 1MultipleXXX
161CMI_A protein inhibitor of neuronal nitric oxide PDB1CMIAX
17HU ciliary dynein heavy chain 9AAF69004X
18IC1 HomologAAG28497XX
19CAB71195 DJ111C20.1 (similar to chlamydomonas radial spoke protein)CAB71195X
20DJ412I7.1 (similar to radial spokehead protein) (fragment)CAC00771X
21Intermediate dynein chainCAC17464X
22Axonemal dynein light chainO14645XX
23Probable dynein protein (fragment)O43352XXX
24Hsp89-α-Δ-NO75322X
25Type II peroxiredoxin 1O88376X
26HU heat shock 27-kDa proteinP04792X
27cAMP-dependent protein kinase, α-catalytic subunitP27791X
28Human Serine/Threonine protein phosphatase 2A, 65-kDa regulatory subunit A, α-isoformP30153XX
29Human transformation-sensitive protein IEF SSP 3521P31948X
30Guanine nucleotide-binding protein G(1), α-2 subunit (adenylate cyclase-inhibiting G α-proteinP38400X
31Kinesin-like protein FLA10 (KHP1 protein)P46869X
32Casein kinase I, α-isoformP48729X
33HU oxygen-regulated protein 1 (retinitis pigmentosa RP1 protein)P56715XX
34HU sperm surface protein Sp17Q15506XXX
35DY13_ANTCR dynein intermediate chain 3, ciliaryQ16960X
36DYL_1CAEEL probable dynein light chain 1 cytoplasmicQ22799X
37Axonemal dynein light chain p33Q26630X
38Dynein heavy chain isotype 3AQ27803XX
39Dynein heavy chain isotype 7AQ27811X
40Cytoplasmic dynein 3 heavy chain (fragment)Q92816X
41β-Dynein heavy chainQ9MBF8X
42Dynein intermediate chain DNAI1Q9UI46X
43KIAA0944 protein (fragment)Q9Y2F3XX
44Axonemal dynein heavy chain protein DNAHQ9Y3I6X
45Brain specific proteinQ9Y6H0X
46Hspc162/bithoraxoidAAF29126XX
47Axonemal dynein heavy chainCAB94756X
48WUGSC:H_RG252K19.1 protein (fragment) (dynein β-chain, ciliary by FASTA)O95705X
49Human calgizzarin (S100C protein)P31949X
50TG737Q13099X
51HU dynein light chain 1, cytoplasmicQ15701X
52Dynein γ-chain, flagellar outer armQ39575X
53Dynein intermediate chain DNAI1Q9UI45X
54Dynein-associated proteinAAK18711X
55Radial spoke protein 3AAK26432X
56Mus Musculus male testis cDNAAK017056X
57Dynein light chain Hp28CAC36087X
58KIAA0357 proteinO15064X
59Sperm flagellar protein SPAG6O75602X
60PP11_DROME Serine/Threonine protein phosphataseP48461X
61FLGK_BUCAI flagellar hook-associated proteinP57428X
62Dynein light chainQ15763X
63DyneinQ9H179X
64Similar to radial spokehead proteinQ9NQ10X
65Dynein heavy chainQ9NYC9X