Table I

46 proteins identified and quantified in mouse neuro2a cells

Swis-Prot accession no.Protein nameMascot hit no.MrProtein concentrationaNumber of observed peptidesMascot scorePAIemPAI
fmoll
P19378 Heat shock cognate 71-kDa protein170,9898222912350.886.56
P07901 Heat shock protein HSP 90-α285,0039403110470.866.26
P20152 Vimentin553,5813362710600.825.58
P58252 Elongation factor 2 (EF-2)696,091118248300.532.41
Q03265 ATP synthase α chain, mitochondrial precursor859,830149186350.562.65
P17182 α enolase947,322596218280.886.50
P15331 Peripherin1054,34984135560.391.48
P48975 Actin, cytoplasmic 1 (β-actin)1242,0531206228941.2215.68
P05213 Tubulin α-2 chain (α-tubulin 2)1450,8181782248621.1412.89
P52480 Pyruvate kinase, M2 isozyme1658,289216176430.492.06
P20001 Elongation factor 1-α 12450,424870176471.009.00
P08113 Endoplasmin precursor2592,70390103790.230.71
O35501 Stress-70 protein, mitochondrial precursor2773,970195154950.381.42
P14869 60 S acidic ribosomal protein P03434,33610293790.502.16
P03975 IgE-binding protein3563,221122103680.331.15
Q9CZD3 Glycyl-tRNA synthetase3782,6247793410.210.62
P35215 14-3-3 protein ζ/δ4027,925381124010.754.62
P42932 T-complex protein 1, θ subunit4260,0889692810.260.81
P51881 ADP, ATP carrier protein, fibroblast isoform4633,13826482940.421.64
Q9JIK5 Nucleolar RNA helicase II4894,1513082680.160.45
P14148 60 S ribosomal protein L75231,45712092270.532.38
Q9WVA4 Transgelin 26523,81013082580.623.12
P14211 Calreticulin precursor7248,13611482460.331.15
P16858 Glyceraldehyde-3-phosphate dehydrogenase8735,94140082700.532.41
P29314 40 S ribosomal protein S98822,41813562010.461.89
Q60932 Voltage-dependent anion-selective channel protein8932,5027262610.381.37
P17080 GTP-binding nuclear protein RAN9724,57925551810.451.85
P17008 40 S ribosomal protein S169816,41845661740.602.98
Q60930 Voltage-dependent anion-selective channel protein9932,3405441610.270.85
P11983 T-complex protein 1, α subunit B10060,8673661430.180.52
P05064 Fructose-bisphosphate aldolase A10339,65621062600.331.15
P09058 40 S ribosomal protein S810924,3449661860.602.98
Q01320 DNA topoisomerase II, α isozyme143173,5673641250.050.12
Q8VEM8 Phosphate carrier protein, mitochondrial precursor14940,0634841220.190.55
P19253 60 S ribosomal protein L13a15023,4324841230.331.15
P08526 60 S ribosomal protein L2715715,6578631130.502.16
P47961 40 S ribosomal protein S416029,66616241090.210.62
Q06647 ATP synthase oligomycin sensitivity conferral protein17923,440783980.230.70
Q9CPR4 60 S ribosomal protein L1718221,506903960.301.00
P39026 60 S ribosomal protein L1118620,3371083920.331.15
Q9D1R9 60 S ribosomal protein L3420413,381963830.502.16
O08807 Peroxiredoxin 420631,261724830.270.85
Q62188 Dihydropyrimidinase related protein-320762,296303820.100.27
P50310 Phosphoglycerate kinase21344,776303800.130.33
Q9DBJ1 Phosphoglycerate mutase 122328,7971143700.250.78
P11442 Clathrin heavy chain226193,187553690.040.10
  • a Protein concentrations were measured by “reversed” isotope dilution using SILAC-labeled proteins and unlabeled synthetic peptides.