In Brief We performed phosphoproteomics after acid guanidinium thiocyanate–phenol–chloroform (AGPC) isolation for cells and tissue and compared it with a classical urea lysis protocol. For both, we show high similarity of data in steady state and after DNA damage induction through irradiation of U2OS cells. Since AGPC extraction can also yield DNA and RNA for molecular characterization, our results could be of potential use in situations when sample material is limited such as in clinical biopsy workflows.
