If you don't remember your password, you can reset it by entering your email address and clicking the Reset Password button. You will then receive an email that contains a secure link for resetting your password
If the address matches a valid account an email will be sent to __email__ with instructions for resetting your password
Division of Analytical and Environmental Toxicology, Department of Laboratory Medicine and Pathology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, CanadaKey Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian, China
Orthogonal IP_MS assays revealed four distinct isoforms of TMPRSS2-ERG fusion protein
T1E4-ERG isoform accounted for 52% of total ERG in VCaP cells, 50% in PCa tissues
Unique N-terminal modifications of T1/E4 TMPRSS2-ERG fusion protein were identified
C-term mAb revealed 29 ERG-interacting proteins including canonical SWI/SNF complexes
TMPRSS2-ERG gene fusion, a molecular alteration found in nearly half of primary prostate cancer cases, has been intensively characterized at the transcript level. However limited studies have explored the molecular identity and function of the endogenous fusion at the protein level. Here, we developed immunoprecipitation–mass spectrometry assays for the measurement of a low-abundance T1E4 TMPRSS2-ERG fusion protein, its isoforms, and its interactome in VCaP prostate cancer cells. Our assays quantified total ERG (∼27,000 copies/cell) and its four unique isoforms and revealed that the T1E4-ERG isoform accounted for 52 ± 3% of the total ERG protein in VCaP cells, and 50 ± 11% in formalin-fixed paraffin-embedded prostate cancer tissues. For the first time, the N-terminal peptide (methionine-truncated and N-acetylated TASSSSDYGQTSK) unique for the T1/E4 fusion was identified. ERG interactome profiling with the C-terminal, but not the N-terminal, antibodies identified 29 proteins, including mutually exclusive BRG1- and BRM-associated canonical SWI/SNF chromatin remodeling complexes. Our sensitive and selective IP-SRM assays present alternative tools to quantify ERG and its isoforms in clinical samples, thus paving the way for development of more accurate diagnostics of prostate cancer.
Prostate cancer is the most frequently diagnosed neoplasm and the third leading cause of cancer mortality in men. Introduction of prostate-specific antigen (PSA) testing revolutionized the practice of urologic oncology (
), facilitated earlier detection of localized tumors, and resulted in the active surveillance as a treatment option for many patients with low-grade prostate cancer. PSA test, however, is prone to overdiagnosis and unable to differentiate between indolent and aggressive cancers (
Recent genomic studies on the primary prostate adenocarcinoma revealed major subtypes defined by gene fusions of E26 transformation-specific (ETS) transcription factors and mutations in SPOP, FOXA1, and IDH1 genes (
). Functionally, TMPRSS2-ERG fusion results in the androgen-dependent overexpression of the N-terminally truncated ERG protein and its isoforms, which may contribute to oncogenic transformation of prostate epithelial cells (Fig. 1A) (
Numerous studies evaluated TMPRSS2-ERG fusion mRNA as a prognostic biomarker, but the fusion mRNA revealed either positive, or negative, or no association with the clinical significance, progression, or aggressiveness of prostate cancer (
). At the protein level, quantification of the total ERG has been suggested as a surrogate marker for the TMPRSS2-ERG fusion. Measurement of ERG protein expression by immunohistochemistry (IHC) in the prostate needle biopsies (
). Antibody-based IHC analysis, however, could not provide any details on the heterogeneity of ERG protein isoforms or posttranslational modifications.
Human ERG gene has 12 predicted protein-coding mRNA isoforms, of which six isoforms were experimentally detected by RNA sequencing in VCaP cells (supplemental Table S1). While the wild-type ERG protein is not expressed in VCaP cells, T1/E4 fusion results in overexpression of the N-term truncated isoforms which retain the function the full ERG. Earlier studies suggested that some ERG isoforms could have distinct molecular functions (
). As a result, isoform-6 protein (P11308-6) lacking DNA-binding ETS domain was suggested as an inhibitor of the transcriptional activation mediated by the full-length ERG isoforms. Overexpression of ERG protein isoforms in HEK293 cells revealed an inhibitory function of isoform-6 protein (
), but expression of the endogenous isoform-6 protein has never been demonstrated in VCaP cells or prostate tissues. In addition, limited experimental data were available for posttranslational modifications (
) is a promising tool to generate novel knowledge on TMPRSS2-ERG heterogeneity at the protein level. He et al. pioneered measurements of ERG protein by targeted mass spectrometry, utilizing two-dimensional liquid chromatography separations and selected reaction monitoring (SRM) assays (
). Unique peptides of the total ERG protein were quantified in VCaP cell lysate, achieving limits of detection of 1.8 fg/cell or ∼3000 cells spiked into urine.
In our study, we aimed at developing immunoprecipitation-shotgun mass spectrometry (IP-MS) and immunoprecipitation-selected reaction monitoring (IP-SRM) assays for the identification and quantification of the endogenous TMPRSS2-ERG fusion protein and its isoforms. As a model cell line, we selected VCaP prostate cancer epithelial cells, which harbored T1/E4 TMPRSS2-ERG gene fusion, overexpressed T1/E4 fusion mRNA, and expressed detectable levels of the endogenous ERG protein. We hypothesized that sensitive IP-MS and IP-SRM assays, as well as orthogonal assays designed with the N-term and C-term monoclonal antibodies, would provide novel knowledge on the abundance of the endogenousTMPRSS2-ERG and its isoforms, TMPRSS2-ERG posttranslational modifications, and TMPRSS2-ERG protein-protein interactions.
Hypothesis, Study Design, and Objectives
We hypothesized that the orthogonal IP-MS assays with the N-term and C-term ERG antibodies will provide novel knowledge on the identity and abundance of ERG isoforms, interactome, and posttranslational modifications. Our study was designed to measure the endogenous T1E4 TMPRSS2-ERG fusion protein in VCaP cells. Specific objectives included: (1) develop IP-MS and IP-SRM assays; (2) quantify the relative abundance of TMPRSS2-ERG protein isoforms in VCaP cells and formalin-fixed paraffin-embedded (FFPE) prostate cancer tissues (exploratory stage), (3) identify the N-term modifications of TMPRSS2-ERG protein, (4) identify TMPRSS2-ERG protein interactome in VCaP cells, and (5) investigate if synthetic N-term epitope peptides could disrupt TMPRSS2-ERG interactome.
Chemicals and Cell Culture Reagents
Iodoacetamide, dithiothreitol, L-methionine, and trifluoracetic acid (TFA) were purchased from Thermo Fisher Scientific. Optima water and acetonitrile were purchased from Fisher Scientiﬁc. Formic acid (FA) was obtained from Sigma-Aldrich. Stable isotope-labeled peptides (SpikeTides_L and SpikeTide_TQL) were obtained from JPT Peptide Technologies GmbH. VCaP and LNCaP prostate cancer lines were obtained from the American Type Culture Collection. Cell lines were cultured in a humidified incubator at 37 °C and 5% CO2. Dulbecco's Modified Eagles Medium (HyClone) and RPMI 1640 medium (Gibco) were used to culture VCaP and LNCaP cells, respectively. Media were supplemented with 10% fetal bovine serum (Invitrogen) and 1% penicillin-streptomycin (Invitrogen).
Cell Lysis and Sample Preparation
Cell pellets were lysed in 50 μl of 0.1% RapiGest SF (Waters) with repeated pipetting, vortexing, and probe sonication at 20 kHz. EDTA-free protease inhibitor cocktail (Roche) combined with Benzonase Nuclease (Fisher Scientific) was added prior to cell lysis to reduce proteolysis and digest nucleic acids. To remove cell debris, lysates were centrifuged at 16,000g and 4 °C for 10 min. Total protein of lysates was measured with Pierce BCA protein assay kit (Fisher Scientific). Proteins were denaturated, and disulfide bonds were reduced by 10 mM dithiothreitol at 70 °C for 15 min and alkylated with 20 mM iodoacetamide at room temperature (RT) in the dark for 45 min. Digestion was completed overnight at 37 °C using recombinant dimethylated SOLu-trypsin (Sigma-Aldrich) with a trypsin:protein ratio 1:20. Trifluoroacetic acid (1%) was added to cleave and precipitate Rapigest SF, and 1 μl of 0.4 M L-methionine was added to limit methionine oxidation during storage. OMIX C18 10 μl tips (Agilent Technologies) were used for desalting and microextraction of tryptic peptides. Finally, samples were diluted in 5% acetonitrile with 0.1% formic acid.
Total RNA was extracted from LNCaP and VCaP cells using TRIzoL (Thermo Fisher Scientific). RNA was reversely transcribed to cDNA via iScriptTM Reverse Transcription Supermix (Bio-Rad Laboratories). After quantification by NanoVue Plus spectrophotometer (GE Healthcare), 500 ng cDNA was utilized as a template for amplification of TMPRSS2-ERG fusion using Hot Start Taq 2X Master Mix (New England Biolabs) and GeneAmp PCR System 2700 thermal cycler (Applied Biosystems). The forward and reverse PCR primers for T1/E4 fusion included 5’-TAGGCGCGAGCTAAGCAGGAG-3’ and 5’-CCATAT TCTTTCACCGCCCACTCC-3’ (Integrated DNA Technologies). ERG isoform-6 primers were 5'-GGTACGAAAACACCCCTGTG-3' (forward) and 5'-CCAAATCAACAGAGGCAGAA-3' (reverse); the total ERG primers were 5'-AACGAGCGCAGAGTTATCGT-3' (forward) and 5'-GTGAGCCTCTGGAAGTCGTC-3' (reverse). The final volume was 25 μl, and an initial denaturation step of 95 °C for 5 min was followed by 40 cycles of 95 °C for 30 s, 59 °C for 30 s, 72 °C for 30 s, and one cycle at 72 °C for 5 min. T1/E4 fusion, isoform-6, and total ERG cDNA were detected by 2% agarose gel electrophoresis (supplemental Fig. S1).
Rabbit monoclonal anti-ERG antibody EPR3864(2) (C-term epitope PNTRLPTSHMPSH; Abcam), mouse monoclonal antibody 9FY (N-term epitope KMSPRVPQQDWLSQ; BioCare Medical), and mouse monoclonal antibody OTI5F12 (epitope unknown; Origene Technologies) were used as capture antibodies for the enrichment of ERG protein from cell lysates (Fig. 1B). Rabbit polyclonal antibody GTX129433 (Genetex) was used to enrich ARI1A_HUMAN. Two microliters of antibody stock was diluted in PBS (pH 7.4), coated (100 μl per well) onto high-binding 96-well polystyrene microplates (#07000128; Greiner Bio-One), and incubated overnight at RT. Since 9FY antibody was provided in Renoir Red solution with carrier proteins, a goat anti-mouse Fcγ fragment-specific antibody (Jackson Immunoresearch Labs) was first coated on plates for 9FY antibody pull-down. After washing with 0.1% Tween 20 in PBS (washing buffer; three times with 200 μl), the plate was blocked for 1 h at RT with 200 μl of blocking buffer (2% BSA in wash buffer). The washing step was repeated, followed by addition of 80 μg total protein VCaP lysates per well and dilution to 100 μl with the dilution buffer (0.1% BSA in wash buffer, 0.2 μm-filtered). After 2 h incubation with continuous shaking, the plate was finally washed three times with 200 μl washing buffer and three times with 50 mM ammonium bicarbonate. Proteins were digested with trypsin (0.25 ng per well). Heavy isotope-labeled peptide internal standards were mixed and diluted to 100 fmol/μl. Three microliters of the internal standard mixture was spiked into each sample before (SpikeTides_TQL peptides) or after (SpikeTides_L peptides) trypsin digestion, and each digest was analyzed in triplicates (10 μl per injection). For the interactome studies, antibody isotype controls included: (i) anti-FOLH1 mouse monoclonal antibody (clone 3B5, Abnova) as an IgG1 isotype control for OTI5F12 and 9FY antibodies; (ii) anti-KLK3 rabbit monoclonal antibody (Sino Biological) as an IgG isotype control for EPR3864(2) antibody.
Selection of Tryptic Peptides and Development of SRM Assays
Peptide Atlas, neXtProt database, and our in-house IP-shotgun MS data were used to select best peptides for the total ERG, or peptides unique and shared by specific isoforms. Excision of the N-term methionine, N-term acetylation, and serine and threonine phosphorylation were selected as potential posttranslational modifications of a unique N-term peptide MTASSSSDYGQTSK of T1/E4 fusion. In total, 14 synthetic heavy isotope-labeled SpikeTides_L peptides were used as internal standards for the Tier 2 SRM assay development (supplemental Table S2). Initially, 28 heavy and light peptide pairs (280 transitions) were included into a multiplex unscheduled SRM method with 5 ms scan time per transition. Based on the SRM peak area, main charge states and collision energies for each peptide were determined, and poorly performing peptides were removed. Low-intensity transitions and transitions with interferences with the VCaP cell lysate were removed. Finally, six best peptides with three or four most intense and reproducible transitions per peptide (42 transitions) were scheduled within 2-min acquisition windows (supplemental Table S3). Scan time of 10 ms ensured acquisition of at least 20 points per peak. Superposition of light and heavy peptide peaks, peak shapes, and the order of y-ion transition intensities ensured the correct identities of peptides in the cell lysate (
). Amounts of the light endogenous peptides were calculated using the peak area ratio of the spiked-in heavy peptide internal standards. To enable accurate and absolute quantification, heavy isotope-labeled SpikeTides_TQL peptides with the trypsin-cleavable JPT-tags [serine-alanine-(3-nitro)tyrosine-glycine] were finally used as internal standards.
Chromatography and Targeted Mass Spectrometry
A quadrupole ion-trap mass spectrometer (AB SCIEX QTRAP 5500) coupled to EASY-nLC II liquid chromatography (Thermo Scientific) via a NanoSpray III ion source (AB SCIEX) was used for SRM assays. The tryptic peptides were loaded at 5 μl/min onto a C18 trap column (Thermo Scientific, 100 μm ID × 2 cm, 5 μm, 120 Å). Peptides were separated with PicoFrit columns (New Objective, 15 cm × 75 μm ID, 8 μm tip, PepMap C18, 3 μm, 100 Å) and 28 min gradients (300 nl/min). The gradient started with 5% buffer B and ramped to 65% buffer B over 20 min, then to 100% buffer B within 1 min, and continued for 7 min. QTRAP 5500 parameters were: 2300 V ion spray; 75 °C source temperature; 2.0 arbitrary units for gas 1 (N2), 0 arbitrary units for gas 2; 25 arbitrary units for curtain gas (N2); and 100 V declustering potential. Q Exactive Hybrid Quadrupole-Orbitrap (Thermo Scientific) coupled to EASY-nLC 1000 (Thermo Scientific) was used for PRM assays. The mobile phase consisted of 0.1% formic acid in water (buffer A) and 0.1% formic acid in acetonitrile (buffer B). Acclaim PepMap 100 nanoViper C18 precolumn (Thermo Scientific, 100 μm ID×2 cm, 5 μm, 100 Å) was used for sample loading, while EASY-Spray C18 (Thermo Scientific, 15 cm × 75 μm ID, 3 μm, 5 μm) was used as an analytical column. An 18-min gradient (400 nl/min) started with 0% buffer B and ramped to 50% buffer B over 15 min, then to 100% buffer B within 1 min, and continued for 2 min. PRM scans were performed at 17.5 K resolution with 27% normalized collision energy. Automatic Gain Control target value was set to 3 × 106 (100 ms injection time; 2.0 m/z isolation width). The performance of the nanoLC-MS systems was assessed every six runs with BSA digest solution (10 μl of 20 fmol/μl).
Chromatography and Shotgun Mass Spectrometry
ERG interactome was identified using Orbitrap Elite Hybrid Ion Trap-Orbitrap mass spectrometer (Thermo Scientific) coupled to EASY-nLC II liquid chromatography. The peptides were eluted at 300 nl/min with a 2-h gradient: 5% B for 5 min, 5 to 35% B for 95 min, 35 to 65% B for 10 min, 65 to 100% B for 1 min, and 100% B for 9 min. LC-MS/MS data were acquired with XCalibur (v. 2.2). MS1 scans (400–1250 m/z) were performed at 60 K resolution in the profile mode, followed by top 20 ion trap centroid MS/MS, acquired at 33% normalized collision energy. Ion counts were set to 1 × 106 (FTMS; 200 ms injection time) and 9000 (MS/MS; 100 ms injection time). MS/MS acquisition settings included 500 minimum signal threshold, 2.0 m/z isolation width, 10 ms activation time, and 60 s dynamic exclusion. Monoisotopic precursor selection and charge state rejection were enabled; +1 and unknown charge states were rejected. Instrument parameters included 230 °C capillary temperature, 2.0 kV source voltage, and 67% S-lens RF level.
IP-SRM Assessment of Interactome Disruption by Synthetic 9FY Epitope Peptides
Synthetic 9FY epitope peptides (HCl salts; Biomatik) included the minimal (RVPQQDWL) and extended (KMSPRVPQQDWLSQ) epitopes. The C-term antibody EPR3864(2) (1 μg per well) was used to enrich ERG interactome from VCaP lysate (80 μg total protein). Following ERG interactome enrichment on 96-well plates (in analytical duplicates), 9FY epitope peptides were added at 0, 200, 400, and 800 μM (in 100 μl PBS) and incubated overnight at RT, followed by three washings and trypsin digestion (0.25 ng per well). SpikeTides_L peptides (200 fmol per well) were used for the accurate relative quantification of seven ERG-interacting proteins (supplemental Table S4). Each analytical replicate was analyzed in technical duplicates using the Tier 2 PRM assays and Q Exactive mass spectrometer.
Lysis and Sample Preparation of Prostate Cancer FFPE Tissues
Thirteen FFPE prostate cancer prostatectomy tissue blocks (ten TMPRSS2-ERG positive and three negative) were obtained from the University of Calgary (Ethics approval #HREBA.CC-14-0085). TMPRSS2-ERG positive areas were marked based on ERG IHC staining at Alberta Precision Laboratories, as previously reported (
). Fifteen 10 μm slices were cut from each block, deparaffinized with toluene (three times for 5 min), centrifuged at 14,000 rpm, and rehydrated with 100% ethanol for 2 min, then 95% ethanol for 2 min, 70% ethanol for 2 min, and distilled water for 1 min. Protocol for tissue pellet homogenization, antigen retrieval, and protein extraction included: (i) 90 min incubation at 90 °C in 0.2% Rapigest SF (100 mM tris-HCl, pH 8) and 1 mM DTT, (ii) repeated probe sonication at 45 kHz for 2 min, (iii) repeated incubation at 90 °C for 90 min; and (iv) centrifugation at 14,000 rpm. One sample (C13) could not be well homogenized, while the limited amount of sample C33 was sufficient only for the IP-SRM measurements.
). Briefly, C-term EPR3864(2) mAb (500 ng in 100 μl PBS per well) was coated onto a 96-well plate and incubated overnight at RT. The plate was washed six times with 250 μl wash buffer (125 mM Tris, 22.5% NaCl, 1.25% Tween 20, pH 7.8) using microplate washer (Biotek ELx50), followed by 1 h blocking (6% BSA in 250 μl wash buffer), and subsequent washing. To generate a calibration curve (7.8–1000 pg/ml), VCaP lysate with the known amount of total ERG (as measured by SRM) was diluted in assay diluent (6% BSA, 0.6% Tris, 20 mM KCl, 0.25% Tween 20). Twelve FFPE sample lysates were diluted in assay diluent (1:5 and 1:20). Calibrants and samples (100 μl per well) were incubated for 2 h with continuous shaking. Detection antibody OTI5F12 was biotinylated in-house with EZ-Link Sulfo-NHS-LC-LC-Biotin (Thermo Scientific, A35358). OTI5F12 (40 ng per well; in 100 μl blocking buffer) was incubated for 1 h. After washing, alkaline phosphatase-conjugated streptavidin (1 mg/ml; Jackson Immuno-Research) diluted in blocking buffer (1:1000 final) was added and incubated for 30 min at RT with continuous shaking. Following the final wash, 100 μl of 10 mM diflunisal phosphate (Toronto Research Chemicals) diluted in substrate buffer (10 mM Tris, 150 mM NaCl, 2 mM MgCl2) was added to each well. After 10 min incubation, 100 μl developing solution (1 M Tris, 0.12 M NaOH, 2 mM Tb3+, 3 mM EDTA) was added. Following 2 min shaking, luminescence of Tb-diflunisal-EDTA complex was measured with FilterMax F5 multi-mode microplate reader (Molecular Devices) with excitation at 370 nm (0.1 ms; TRF excitation filter 370NM BW80) and emission at 625 nm (0.1 ms delay and 0.6 ms integration time; fluorescence emission filter 625NM BW35).
Experimental Design and Statistical Rationale
For the preliminary quantification of the TMPRSS2-ERG protein isoforms, VCaP cell lysates were subjected to IP by the C-term, N-term, and 5F12 mAbs, or matched no-Ab controls, and were analyzed by the single-point titration with 100 fmol/injection SpikeTides_TQL heavy peptide internal standards, three analytical replicates, and two technical replicates (36 injections in total). According to effect size calculations, IP-SRM analysis with three analytical replicates could detect at least 17% change in ERG peptide abundance, respectively, assuming 80% power, α = 0.05, average CV 5% for analytical replicates in our IP-SRM experiments, and a two-tailed paired t-test (G∗Power software, v3.1.7, Heinrich Heine University Dusseldorf). For more accurate titration, VCaP cell lysates were subjected to IP by the C-term and N-term mAbs and were analyzed with 1, 5, and 20 fmol/injection SpikeTides_TQL standards, two analytical replicates, and two technical replicates (24 injections in total). To measure ERG and its isoforms in clinical samples (exploratory stage), 13 FFPE samples (ten positive and three negative for TMPRSS2-ERG fusion) were selected. Due to the low amounts of tissue, only one analytical and two technical replicates were analyzed per sample. For the interactome study, VCaP cell lysates were subjected to IP by the C-term, N-term, and 5F12 mAbs, or matched isotype mAb controls, and were analyzed with three analytical and two technical replicates (36 injections). According to effect size calculations, IP-MS analysis with three analytical replicates could identify proteins up- or down-regulated at least 1.09-fold, assuming 80% power, α = 0.05, average 2.7% coefficient of variation (CV) for log2-transformed LFQ (Label-Free Quantification with MaxQuant software) values and a two-tailed t-test (G∗Power software, v3.1.7, Heinrich Heine University Dusseldorf).
MS Data Analysis
SRM and PRM raw files were analyzed with Skyline Targeted Proteomics Environment v184.108.40.206 (MacCoss Lab) (
). Peak boundaries were adjusted manually, and the integrated areas of all transitions were extracted. Light-to-heavy peak area ratios were used for accurate relative or absolute quantification of the endogenous peptides. Shogun MS data were searched against the reviewed human UniProtKB/Swiss-prot database (20,365 entries, Uniprot release 2020_03) using MaxQuant software (v220.127.116.11) (
). Search parameters included: trypsin enzyme specificity, two missed cleavages, 7 aa minimum peptide length, top eight MS/MS peaks per 100 Da, 20 ppm MS1, and 0.5 Da MS/MS tolerance. Fixed modifications included cysteine carbamidomethylation; variable modifications included methionine oxidation, N-terminal acetylation, and deamidation (N). False-discovery rates for peptide and protein identifications were set to 1.0%. Label-free quantification (LFQ) algorithm was used for protein quantification. MaxQuant search results file proteinGroups.txt was uploaded to Perseus software (v18.104.22.168) to complete statistical analysis and data visualization (
). Protein identifications marked as “Only identified by site,” “Reverse,” and “Contaminants” were excluded. LFQ intensities were log2-transformed, and missing LFQ values were imputed with values representing normal distribution (0.2 width and 1.8 down shift). Proteins significantly enriched by anti-ERG antibodies versus isotype controls were selected using the log2 fold cutoffs (one-tail t-test p-value < 0.05). In addition, IP-shotgun MS data was searched against the custom database with all potential noncanonical ERG isoforms (total 46 unreviewed TrEMBL ERG isoforms and 20,365 entries of the reviewed human Swiss-Prot release 2020_03).
Assessment of Synthetic 9FY Epitope Peptides in Live VCaP Cells
To facilitate cell penetration and investigate synthetic 9FY epitope peptides in live VCaP cells, RVPQQDWL and KMSPRVPQQDWLSQ peptides were synthesized with the TAT sequence (GRKKRRQRRRG) at N- and C- terms (HCl salts; Biomatik). Peptides (final 400 μM in PBS) were incubated with VCaP cells (15,000 cells per well) on a 96-well plate. After 4 days of incubation, cell morphology was monitored via live cell imaging using the MetaXpress XLS system (Molecular Devices). Cell confluency was estimated with cell counting (average of four images) by the FIJI cell segmentation algorithm (ImageJ v1.51w).
Selection of ERG Protein Isoforms
RNA sequencing data available at the Cancer Cell Line Encyclopedia (CCLE) and DeepMap database (v2; https://depmap.org (
)) revealed that only six protein-coding mRNA isoforms were expressed in VCaP cells (supplemental Table S1). Our rationale for consideration of the endogenous ERG isoforms was based on the following facts: (i) VCaP cells had four copies of chromosome 21 (
), of which only two harbored T1/E4 TMPRSS2-ERG fusion and expressed TMPRSS2-ERG mRNA and a truncated ERG protein; (ii) similar to other prostate cancer cells, wild-type chromosomes 21 did not express any full length ERG (supplemental Table S5 and supplemental Fig. S2); (iii) mRNA T1/E4 isoforms 1 and 2, as well as isoforms 3 and B5MDW0, were identical at the protein level (Table 1); (iv) protein-coding mRNA isoforms 5, A0A088AWP2, A8MX39, and A8MZ24 were not expressed in VCaP cells. As a result, we considered five protein isoforms for further investigation (Table 1).
Table 1TMPRSS2-ERG protein isoforms identified and quantified in VCaP cells
To measure the expression and relative abundance of ERG protein isoforms, tryptic peptides for the total ERG, peptides shared by some isoforms, or peptides unique for specific isoforms were selected. Among five protein isoforms, only T1E4-ERG_7bpA had unique peptides (Fig. 1C). The remaining four isoforms could be distinguished using combinations of the shared tryptic peptides VPQQDWLSQPPAR, NTDLPYEPPR, and ITTRPDLPYEPPR (Fig. 1C). Since isoforms T1E4-ERG_Δ4Δ7b and T1E4-ERG_Δ4 were lacking the N-term exon 4 (including KMSPRVPQQDWLSQ epitope of the 9FY mAb), immunoprecipitation with either the N-term 9FY or C-term EPR3864(2) antibodies followed by quantification of the shared and total ERG peptides would unambiguously resolve relative abundances of these four ERG isoforms.
Quantification of TMPRSS2-ERG Protein Isoforms by Orthogonal IP-SRM Assays
ERG protein was immunoprecipitated from VCaP cell lysate with three different anti-ERG mAbs. Following trypsin digestion, ERG protein was quantified by SRM using trypsin-cleavable SpikeTides_TQL internal standard peptides (supplemental Table S6), to enable absolute quantification (Fig. 2A). Figure 2B shows extracted-ion chromatograms of transitions monitored for the unique ERG peptides in VCaP lysate. ERG peptides detected in VCaP lysate by three different antibodies are listed in Table 2 and supplemental Table S7. The recovery of ERG after IP was estimated at ∼90% (relative to the total ERG measured in the direct digest of VCaP cells). IP-SRM assays revealed excellent reproducibility, with coefficient of variation (CV) below 10% (Table 2). Limit of detection of IP-SRM, as estimated with serial dilutions of VCaP lysate, was 93.6 amol/μg total protein or ∼10,000 cells (supplemental Fig. S3). Three antibodies displayed similar efficiency of capturing total ERG protein (69–84%; Fig. 2C and supplemental Table S8). Based on the cell count, the amount of total ERG was estimated at 2.2 fg per VCaP cell (∼27,000 copies/cell), which was in good agreement with the previously reported levels (1.8 fg per cell) (
Table 2Quantification of TMPRSS2-ERG isoform razor peptides by IP-SRM in VCaP cells
Shared and unique peptides
C-term mAb; L/H ratio (CV%)
N-term mAb; L/H ratio (CV%)
Total ERG (all five isoforms)
T1E4-ERG; T1E4-ERG_Δ7b; T1E4-ERG_7bpA
The amounts (L/H ratios) and coefficients of variation (CV) were calculated based on analytical duplicates with spiked heavy standards of 1, 5, and 20 fmol per injection. Detailed data are presented in supplemental Table S9.
While mRNA isoform-6 was expressed at high levels (supplemental Fig. S4), none of the unique peptides of the corresponding protein isoform T1E4-ERG_7bpA were detected in VCaP cells (supplemental Fig. S5). Detection of the mutually exclusive peptides ITTRPDLPYEPPR and NTDLPYEPPR indicated expression of at least two distinct ERG isoforms (Fig. 2B). Based on our data (Table 2, supplemental Table S9, and supplemental Text), the relative abundance of four ERG isoforms was estimated at 52.3 ± 2.7% (T1E4-ERG), 9.5 ± 1.0% (T1E4-ERG_Δ7b), 32.9 ± 2.1% (T1E4-ERG_Δ4), and 5.3 ± 2.3% (T1E4-ERG_Δ4Δ7b). To the best of our knowledge, the relative abundance of endogenous ERG isoforms, as well as lack of expression of the shortest isoform T1E4-ERG_7bpA (P11308-6) missing ETS domain was quantified for the first time.
Identification of the N-terminal Peptide of T1/E4 TMPRSS2-ERG Protein
Tryptic digestion of T1E4-ERG and T1E4-ERG_Δ7b isoforms (∼62% of the total ERG in VCaP cells) should generate a unique N-terminal peptide MTASSSSDYGQTSK (as opposed to the wild-type ERG peptide TEMTASSSSDYGQTSK). However, this unique peptide has never been identified in previous studies (
). We hypothesized that the N-terminus of ERG could be further modified by acetylation, methionine cleavage, or threonine and serine phosphorylation. The challenge to detect a low-abundance fusion-specific peptide was our initial motivation. We then realized that the knowledge of the N-terminal modifications of ERG could be important to explore a presumably increased stability of TMPRSS2-ERG protein (
) of the fusion-derived, but not the wild-type ERG.
To reveal the N-terminal identity of T1/E4 TMPRSS2-ERG protein, we searched IP-MS/MS data for peptides modified with the N-terminal acetylation, N-terminal methionine truncation, and phosphorylation of threonine, serine, and tyrosine. Our search identified a peptide N-acetyl-TASSSSDYGQTSK, with the near-complete series of y- and b-fragment ions (Fig. 3A). Additional search of unreviewed TrEMBL sequences did not provide any nonstandard isoforms. We then confirmed the N-terminal identity of the T1/E4 TMPRSS2-ERG protein with more sensitive IP-SRM assays using internal standards with several potential modifications (Fig. 3B). IP-SRM confirmed the presence of N-acetyl-TASSSSDYGQTSK peptide (Fig. 3C). No phosphorylation of threonine or serine, as opposed to previous studies on ERG overexpressed in 293T cells (
We hypothesized that orthogonal IP-MS/MS assays with the N-term and C-term antibodies could elucidate the most complete interactome of the endogenous ERG. First, we optimized conditions for the mild cell lysis. Several nondenaturing detergents (
), including (i) 0.1% Rapigest; (ii) modified IP lysis buffer (0.1% Rapigest, 1% NP-40, 0.1% sodium deoxycholate), and (iii) 0.5% SDS with 0.5% NP-40 were tested, and 0.1% Rapigest was selected based on the completeness of the cell lysis, total protein amount, and ERG recovery.
Following concurrent immunoprecipitation with the N-term, C-term, and 5F12 mAbs, ERG interactomes were identified by shotgun MS with the label-free quantification. MaxQuant search resulted in identification and quantification of 449 proteins (supplemental Table S10). As opposed to shotgun MS of the VCaP direct digest (no ERG identified), IP-MS identified ERG with eight unique peptides, including some isoform-specific peptides (supplemental Fig. S6). Proteins identified with LFQ fold change >1.3 and p-value <0.05 were selected as ERG-interacting proteins (Fig. 4 and supplemental Table S11). As a result, IP-MS with the C-term antibody identified 29 ERG-interacting proteins, including canonical BAF (SWI/SNF) chromatin remodeling complex subunits, androgen receptor (
), and nuclear receptor co-activators NCOA2_HUMAN and NCOA6_HUMAN (Fig. 4A and supplemental Fig. S7). Identification of eight proteins of the canonical BAF complexes (∼2000 kDa polymorphic assemblies (
). Interestingly, unique protein subunits of the polybromo-associated BAF complexes (including a unique subunit ARID2_HUMAN), noncanonical BAF (BRD9_HUMAN), embryonic stem cell-specific BAF (BC11A_HUMAN), or neuron-specific BAF (ACTL6B_HUMAN) were not identified in the ERG interactome, thus suggesting that expression of ERG-regulated genes in VCaP cells was mediated through the canonical BAF complexes, including mutually exclusive BRG1 (SMARCA4)- and BRM (SMARCA2)-associated BAF complexes (
). We believe that our C-term IP-MS identified one of the most comprehensive ERG interactomes (supplemental Table S11).
Interestingly, while ERG protein alone has been efficiently enriched 81-fold (N-term mAb, supplemental Table S12), 67-fold (C-term mAb), and 48-fold (5F12 mAb), immunoprecipitation with the N-term mAb and 5F12 mAb provided mostly high-abundance ribosomal, mitochondrial, and housekeeping proteins (most common contaminating proteins (
)), but failed to provide any meaningful candidates, such as proteins involved in transcriptional regulation (Fig. 4, B and C). We believe that such difference was due to the antibodies specificity, rather than stochastic nature of co-IP-MS. For example, detailed examination of MS1 data for ARI1A_HUMAN protein, the subunit of the canonical BAF complexes, revealed the high reproducibility of the C-term IP-MS assays and confirmed the absence of ARI1A_HUMAN in the IP experiments with N-term mAb and 5F12 mAb (supplemental Fig. S8). Such unexpected result on interactome differences suggested that some antibodies could interfere or disrupt protein–protein interactions of ERG. We previously observed similar antibody-mediated disruptions of protein–protein interactions for the TEX101-DPEP3 complex (
). Here, this finding motivated us to investigate further if the ERG interactome could be disrupted by short synthetic peptides representing the binding epitope of the N-term mAb, thus paving the way for development of potential disruptors of the oncogenic ERG interactomes.
Independent Verification of ERG-Interacting Proteins by PRM
To verify some ERG-interacting proteins, we developed parallel reaction monitoring (PRM) assays for the most interesting ERG-interacting proteins, including some cBAF complex subunits (ARI1A_HUMAN, SMCA2_HUMAN, and SMCE1_HUMAN), androgen receptor (ANDR_HUMAN), nuclear receptor coactivators (NCOA2_HUMAN and NOCA6_HUMAN), and a transcriptional coregulator EWS. Targeted assays with the heavy peptide internal standards facilitated precise measurements of protein relative abundances and more accurate estimation of differential enrichments, as previously demonstrated (
). Interestingly, independent verification revealed potentially three groups of ERG-interacting proteins: 1) strong and moderate ERG binders disrupted by the N-term antibody (NCOA2_HUMAN, NCOA6_HUMAN, and ARI1A_HUMAN); 2) a strong ERG binder not disrupted by the N-term antibody (EWS_HUMAN); and 3) weak ERG binders (supplemental Table S12).
It should be noted that while additional experiments with reciprocal IP-SRM and polyclonal anti-ARI1A antibodies successfully enriched ARI1A_HUMAN (110-fold versus isotype control), neither total ERG or its isoforms were detected. That result could be explained by the lower sensitivity of the ERG peptide assays, saturation of polyclonal anti-ARI1A antibodies with ARI1A_HUMAN paralogs (VCaP cells expressed six proteins of the AT-rich interactive domain-containing family (
), with 30–60% sequence identity), or lower stability of massive cBAF complexes (∼2000 kDa) during IP-SRM. Overall, protein–protein interactions of cBAF are heterogeneous and dynamic; cBAF complexes simultaneously interact with numerous transcriptional activators. Our ERG C-term IP-SRM data estimated that only 1 in 50 ERG molecules interacted with ARI1A_HUMAN. We could suggest that the reciprocal IP-SRM assays for verification of cBAF interactions might utilize mAbs generated against known and unique epitopes with no cross-reactivity to the numerous paralogs of cBAF subunit proteins.
Evaluation of ERG Interactome Disruption by the N-term Epitope Peptides
Since the N-term mAb could potentially disrupt ERG interactome, we hypothesized that synthetic peptides representing minimal or extended epitopes (RVPQQDWL and KMSPRVPQQDWLSQ (
), respectively) could also disrupt interactions between ERG protein and its interactome.
First, we confirmed by IP-PRM that both peptides had no effect on ERG enrichment by the C-term mAb, but disrupted ERG enrichment by the N-term mAb. Following that, we evaluated the impact of a minimal epitope peptide RVPQQDWL on co-IP of some ERG-interacting proteins. As a result, only small changes were observed at very high RVPQQDWL concentrations (800 μM; Fig. 5A, supplemental Table S13). We concluded that the minimal epitope peptide was not a strong candidate for the disruption of ERG-interacting proteins (EC50 in mM range, if any at all).
Impact of the N-term Epitope Peptides on Proliferation and Morphology of VCaP Cells
We also evaluated the impact of a minimal epitope peptide RVPQQDWL on proliferation and morphology of VCaP cells. The cell-permeable N-term epitope peptides were conjugated to the cationic HIV-TAT motif GRKKRRQRRRG, to facilitate uptake into VCaP cells (
). As a result, we found that the cell-permeable N-term epitope peptides (400 μM) had no impact on VCaP proliferation (Fig. 5B, supplemental Table S14) or morphology (Fig. 5C).
Measurement of TMPRSS2-ERG Isoforms in Prostate Cancer FFPE Tissues by IP-SRM Assays
Quantification of four distinct TMPRSS2-ERG isoforms in VCaP cells motivated us to explore isoform identity in prostate cancer clinical samples. While FFPE tissues appear as the most challenging samples for proteomics, FFPE samples are widely available, have detailed clinical information, and include large amounts of tissue (radical prostatectomy FFPEs). Here, we obtained radical prostatectomy FFPEs blocks with positive (N = 10) and negative (N = 3) tissues for TMPRSS2-ERG fusion (as assessed by ERG IHC staining), and developed an IP-SRM-compatible protocol for protein extraction and quantification. Both C-term and N-term mAbs could enrich ERG protein even after intense tissue homogenization, probe sonication, and rigorous protein denaturation at high temperature in the presence of detergents.
As a result, total ERG protein was quantified by IP-SRM in six and ten fusion-positive FFPEs, with C-term and N-term mAbs, respectively (Fig. 6, A and B). LOD of the N-term IP-SRM (0.39 fmoles on column; S/N = 3) was low enough to differentiate between fusion-positive and negative FFPEs. Median ERG levels in ERG-positive tissues were 1.5 [IQR 1.2–1.6] fmoles on column. In addition, our C-term and N-term IP-SRM were sensitive enough to measure low-abundance isoform-specific peptides in five FFPE samples (Fig. 6B and supplemental Table S15). Interestingly, IP-SRM with the N-term mAb could also detect a low-abundance fusion-specific peptide Ac-TASSSSDYGQTSK in three fusion-positive FFPEs. Collectively, our data (Fig. 6) revealed that T1E4-ERG was the dominant isoform both in VCaP cells (52 ± 3%) and prostate-cancer FFPE tissues (50 ± 11%). These new data on expression of distinct ERG isoforms warrant further investigation on their association with progression and aggressiveness of prostate cancer.
Measurement of TMPRSS2-ERG in FFPE Tissues by TRF-ELISA
To facilitate independent validation of IP-SRM data in FFPE tissues, we developed in-house a highly sensitive (60 pg/ml LOD) and reproducible time-resolved fluorescence ELISA using the C-term and 5F12 mAbs for ERG capture and detection, respectively. TRF-ELISA revealed ERG levels below LOD in three ERG-negative FFPE lysates, and ERG levels ranging from 0.4 to 2.7 ng/ml in eight ERG-positive FFPE lysates (Fig. 6A, supplemental Fig. S9 and supplemental Table S16).
It should be noted that colorimetric ELISA for ERG protein (300 pg/ml LOD) has previously been reported (
), with the N-term mAb (9FY) used as a capture antibody (lacking detection of Δ4 ERG isoforms: T1E4-ERG_Δ4 and T1E4-ERG_Δ4Δ7b). Even though the epitope of 5F12 mAb was not known, our IP-SRM data suggested that 5F12 enriched Δ4 ERG isoforms (based on VPQQDWLSQPPAR/VIVPADPTLWSTDHVR ratios of 0.85 ± 0.04, 0.78 ± 0.01, and 1.28 ± 0.02 for the C-term, 5F12, and N-term mAbs, respectively; supplemental Table S7). In future, our ELISA and IP-SRM assays may facilitate development of ERG isoform-specific ELISA and evaluate clinical significance of total ERG (ELISA with the C-term capture and 5F12 detection mAbs) versus clinical significance of isoforms T1E4-ERG and T1E4-ERG_Δ7b (ELISA with the N-term capture and 5F12 detection mAbs).
Proteomics by mass spectrometry advanced to the level of identification and quantification of nearly whole proteomes of human cells (∼12,000 proteins per cell) (
). However, quantification of low-abundance cellular proteins, such as transcription factors, may still require extensive sample fractionation or protein enrichment approaches. Distinct isoforms of transcription factors may present particular interest due to their different, or even opposite, molecular functions (for example, dominant-negative effects of the N-terminally truncated isoform ERα46 of the estrogen receptor alpha (
). Immunoprecipitation substantially reduces sample complexity and facilitates quantification of multiple peptides per protein, thus enabling resolution of splicing isoforms and in-depth analysis of posttranslational modifications. In our previous studies, IP-SRM assays were successfully utilized to quantify low-abundance kallikrein-related peptidases (
). In this study, we focused on development of IP-MS and IP-SRM assays for a low-abundance fusion protein TMPRSS2-ERG. While the TMPRSS2-ERG gene fusion was found in nearly 50% of prostate cancer cases (
)) is still conflicting. Here, we hypothesized that IP-MS and IP-SRM would emerge as novel assays to characterize TMPRSS2-ERG fusion protein, quantify its levels in cells and tissues, elucidate its N-terminal posttranslational modifications, resolve the identity and abundance of TMPRSS2-ERG protein isoforms, and discover TMPRSS2-ERG interactome.
Wild-type ERG is a transcription factor expressed in endothelial cells and implicated in vascular development and angiogenesis (
). ERG expression is negligible in normal prostate tissues and prostate epithelial and stromal cells. Only two prostate cancer cell lines harbor TMPRSS2-ERG gene fusion and express measurable amounts of ERG transcripts: VCaP (∼50,000-fold higher expression versus baseline expression in prostate stromal cells) and NCI-H660 (∼6,000-fold) (
), ERG isoforms still cannot be fully resolved without extensive fractionation or protein enrichment approaches. He et al. previously pioneered quantification of ERG protein with two-dimensional liquid chromatography and targeted SRM assays (
). Here, we resolved for the first time the relative abundance of four ERG isoforms in VCaP cells and demonstrated that isoform-6 was not expressed at the protein level (supplemental Figs. S4 and S5).
Recently, it has been demonstrated that degradation of wild-type ERG protein was mediated through the SPOP ubiquitin ligase-binding site 42-ASSSS-46 (“degron”) located at the N-term of ERG. The N-term truncated TMPRSS2-ERG fusion protein displayed significantly reduced interaction with SPOP in vivo and in vitro. (
) This fact encouraged us to characterize the N-terminal composition of the endogenous TMPRSS2-ERG fusion protein in VCaP cells. Our sensitive IP-SRM assays revealed the N-term methionine truncation and threonine acetylation (peptide N-acetyl-TASSSSDYGQTSK), but no phosphorylation of threonine or serine. In future, development of affinity ligands recognizing the N-terminal motif N-acetyl-TASSSS (none of the human canonical UniProt protein isoforms have the N-term motifs MTASSSS or MTASSS) could be utilized as strategy to target TMPRSS2-ERG fusion protein for degradation (
Finally, we employed our orthogonal IP-MS assays to identify interactome of the endogenous TMPRSS2-ERG protein in VCaP cells. Previous co-IP-MS studies completed with polyclonal antibodies identified partial ERG interactomes (lacking BAF complex subunits (
Our data revealed that the N-term and 5F12 mAbs could enrich ERG, but not its interactome. We suggested that these high-affinity mAbs could interfere with ERG protein–protein interactions, and that the region crucial for interactions with cBAF complexes could be located between the N-term epitope (46-KMSPRVPQQDWLSQ-59 of P11308-4) and the DNA-binding ETS domain (aa 320–391). PNT domain (aa 120–199) located in that region was previously suggested as the protein–protein interaction domain of the ETS family of transcription factors (
). Our experimental methods, however, were lacking any three-dimensional structural perspectives to explore the hypothesis that interactions of large cBAF complexes (∼2000 kDa) and transcriptional regulators (NCOA2_HUMAN and NCOA6_HUMAN, ∼200 kDa) could be disrupted due to steric hindrance with the N-term or 5F12 mAbs. In future, the exact regions of ERG protein–protein interactions could be identified with the series of recombinant truncated ERG proteins, epitope mapping arrays, or cross-linking mass spectrometry (
). It would also be worth investigating whether the shorter isoforms T1E4-ERG_Δ7b and T1E4-ERG_Δ4Δ7b (lacking exon 7 of 26 aa in the region between the N-term epitope and ETS domain; representing 15% of total ERG) could reveal alternative interactomes or exhibit any dominant-negative inhibition of the ERG transcriptional activity (similar to the hypothetical isoform T1E4-ERG_7bpA (
), could be identified and explored as potential targeted therapies of prostate cancer.
Finally, we demonstrated that our IP-SMR assays were useful for quantification of ERG and its four isoforms in prostatectomy FFPE tissues. Sensitivity of the N-term IP-SRM was sufficient to differentiate between fusion-positive FFPEs (median ERG levels 1.5 [IQR 1.2–1.6] fmoles on column) and fusion-negative FFPEs (values below LOD of 0.39 fmoles on column). These data were in agreement with our in-house TRF-ELISA results for fusion-positive (median 1.2 [IQR 0.8–1.5] ng/ml) and fusion-negative FFPEs (values below LOD of 0.06 ng/ml). In future, our IP-SRM assays could be utilized to quantify ERG and its isoforms in fresh-frozen tissues, semen, and exfoliated prostate epithelial cells in urine. Some post-digital rectal examination urine samples of prostate cancer patients were found to contain up to 27,000 exfoliated cells (
), which was above LOD of our IP-SRM assay (∼10,000 VCaP cells). Our ELISA and IP-SRM assays could also facilitate development of ERG isoform-specific immunoassays and evaluate clinical significance (for example, the risk of prostate cancer progression during active surveillance (
)) of total ERG versus isoforms T1E4-ERG and T1E4-ERG_Δ7b in the large cohorts of clinical samples. Collectively, future applications of our IP-SRM assays may well contribute to the precision diagnostics needs prioritized by the Movember Prostate Cancer Landscape Analysis (
We developed IP-SRM and IP-MS assays for the quantification of a low-abundant transcriptional factor TMPRSS2-ERG fusion protein (∼27,000 copies/cell), its isoforms, and its interactome in VCaP cells. Our orthogonal IP-SRM assays quantified for the first time the relative abundance of four isoforms and revealed that the T1E4-ERG isoform accounted for 52 ± 3% of the total ERG protein in VCaP cells and 50 ± 11% in prostate cancer FFPE tissues. For the first time, the N-terminal peptide (methionine-truncated and N-acetylated TASSSSDYGQTSK) unique for the T1/E4 fusion was identified. ERG interactome mapping with the C-terminal antibodies identified 29 proteins, including the mutually exclusive BRG1- and BRM-associated canonical SWI/SNF chromatin remodeling complexes, and numerous transcriptional regulators. Our sensitive and selective IP-SRM assays present alternative tools to quantify ERG and its isoforms in clinical samples, thus paving the way for development of more accurate diagnostics of prostate cancer.
We thank Prof. Xingfang Li for the access to QTRAP 5500 mass spectrometer and Dr. Konstantin Stoletov for providing VCaP and LNCaP cells.
Funding and additional information
This work was supported by the Prostate Cancer Canada grant (RS2015-01) to A. P. D.; Z. F. acknowledges the support from the National Natural Science Foundation of China (21806018) and Globalink Early Career Fellowship (Mitacs, Canada and China Scholarship Council, China; 201806065018).
A. P. D. designed the research project. Z. F. and Y. R. performed all major experiments and analyzed the data. T. A. B. and M. E. H. provided clinical samples and clinical information. Z. F. and A. P. D. wrote the article, and all the authors contributed to the revisions.
Z. F.: Investigation, Methodology, Validation, Formal Analysis, Writing—Original Draft, Writing—Review and Editing, Data Curation, Visualization, Y. R.: Investigation, Writing—Review and Editing, T. A. B.: Resources, M. E. H.: Resources, X. C. L.: Resources, Writing—Review and Editing, Funding Acquisition, A. P. D.: Conceptualization, Methodology, Validation, Formal Analysis, Data Curation, Writing—Original Draft, Writing—Review and Editing, Visualization, Supervision, Project Administration, Funding Acquisition