- •The red blood cell (RBC) proteins are modified due to the presence of a breast tumor.
- •Embryonic or fetal hemoglobins were found in RBCs from breast cancer patients.
- •LAMP2 from RBCs is a prognostic biomarker in breast cancer patients.
- •RBCs display unexplored functions in the metastatic setting.
Abbreviations:BC (breast cancer), CFC (cancer-free controls), CTCs (circulating tumor cells), GSEA (gene set enrichment analysis), Hb (hemoglobin), M0 (nonmetastatic), M1 (metastatic), MS (mass spectrometry), PBMCs (peripheral blood mononuclear cells), PNP (purine nucleoside phosphorylase), RBCs (red blood cells), RDW (red blood cell distribution width), SWATH-MS (Sequential Window Acquisition of All Theoretical Mass Spectra)
Patients and Samples
|Bone & visceral||16||44.44|
|Number of metastatic sites|
Proteomic Analysis by TripleTOF 6600 LC-MS/MS System
LC-MS/MS in Data-Dependent Acquisition Mode-Shotgun Analysis
Protein Quantification by SWATH-MS Analysis
Differential RBCs Proteomic Profiles Between BC Patients and Cancer-Free Controls
Comparative Analysis Between Shotgun- and SWATH-MS Proteomics Data–Identified Common Proteins
Identification of Nonreported Proteins in RBCs
Metastatic BC Patients Showed Altered Blood Clinical Values
LAMP2 as a Prognostic and Diagnostic Marker for Metastatic BC
- Olumuyiwa-Akeredolu O.-O.O.
- Soma P.
- Buys A.V.
- Debusho L.K.
- Pretorius E.
Conflict of interest
Funding and additional information
- Supplemental Table S1
List of patients and CFC showing concentration of RBCs protein (μg/μl), hematocrit value, and subtype information.
Supplemental Table S2. Summary of numbers of peptides identified from RBCs extracts from pools of cancer-free controls (CFC), M0, and M1 BC patients by shotgun.
Supplemental Tables S3–S5. List of peptides of CFC, M0, or M1 pooled samples from shotgun analysis.
Supplemental Table S6. Library of proteins identified by SWATH-MS in CFC samples.
Supplemental Table S7. List of differentially expressed proteins by SWATH-MS.
Supplemental Table S8. List of potential novel proteins identified by Swath-Ms or shotgun compared with reported RBCs and platelets databases.
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Present address for Thais Pereira-Veiga: Department of Tumor Biology, Center of Experimental Medicine, University Medical Center Hamburg-Eppendorf, Hamburg 20246, Germany.
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