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Molecular & Cellular Proteomics

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Research

Identification of O-Linked N-Acetylglucosamine (O-GlcNAc)-modified Osteoblast Proteins by Electron Transfer Dissociation Tandem Mass Spectrometry Reveals Proteins Critical for Bone Formation

Alexis K. Nagel, Michael Schilling, Susana Comte-Walters, Mary N. Berkaw and Lauren E. Ball
Molecular & Cellular Proteomics April 1, 2013, First published on February 26, 2013, 12 (4) 945-955; https://doi.org/10.1074/mcp.M112.026633
Alexis K. Nagel
From the Departments of ‡Craniofacial Biology and
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Michael Schilling
From the Departments of ‡Craniofacial Biology and
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Susana Comte-Walters
§Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston, South Carolina 29425
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Mary N. Berkaw
§Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston, South Carolina 29425
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Lauren E. Ball
§Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston, South Carolina 29425
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  • For correspondence: ballle@musc.edu
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Abstract

The nutrient-responsive β-O-linked N-acetylglucosamine (O-GlcNAc) modification of critical effector proteins modulates signaling and transcriptional pathways contributing to cellular development and survival. An elevation in global protein O-GlcNAc modification occurs during the early stages of osteoblast differentiation and correlates with enhanced transcriptional activity of RUNX2, a key regulator of osteogenesis. To identify other substrates of O-GlcNAc transferase in differentiating MC3T3E1 osteoblasts, O-GlcNAc-modified peptides were enriched by wheat germ agglutinin lectin weak affinity chromatography and identified by tandem mass spectrometry using electron transfer dissociation. This peptide fragmentation approach leaves the labile O-linkage intact permitting direct identification of O-GlcNAc-modified peptides. O-GlcNAc modification was observed on enzymes involved in post-translational regulation, including MAST4 and WNK1 kinases, a ubiquitin-associated protein (UBAP2l), and the histone acetyltransferase CREB-binding protein. CREB-binding protein, a transcriptional co-activator that associates with CREB and RUNX2, is O-GlcNAcylated at Ser-147 and Ser-2360, the latter of which is a known site of phosphorylation. Additionally, O-GlcNAcylation of components of the TGFβ-activated kinase 1 (TAK1) signaling complex, TAB1 and TAB2, occurred in close proximity to known sites of Ser/Thr phosphorylation and a putative nuclear localization sequence within TAB2. These findings demonstrate the presence of O-GlcNAc modification on proteins critical to bone formation, remodeling, and fracture healing and will enable evaluation of this modification on protein function and regulation.

Advances in proteomic approaches are revealing the abundance of β-O-linked N-acetylglucosamine (O-GlcNAc)1 modification on highly phosphorylated proteins and regulatory enzymes involved in post-translational processing (1, 2). First reported in 1984 in B-lymphocytes (3), O-GlcNAc modification is now appreciated as a nutrient-responsive mechanism for modulating signal transduction (4, 5) and transcriptional regulation (6, 7). In contrast to N- and O-linked glycosylation taking place in the endoplasmic reticulum and Golgi apparatus, reversible O-GlcNAcylation occurs in the cytoplasm and nucleus and is catalyzed by O-GlcNAc transferase (OGT), which transfers GlcNAc from UDP-GlcNAc to Ser/Thr residues, and O-GlcNAcase (OGA), which removes it. These highly conserved enzymes are expressed in all mammalian tissues (8⇓⇓–11).

The OGT gene encodes three splice variants differing in the number of N-terminal tetratricopeptide repeats that mediate protein-protein interactions and subcellular localization (8, 12). The full-length nucleocytoplasmic form of OGT is O-GlcNAc-modified, tyrosine-phosphorylated, and dynamically redistributed within the cell upon insulin stimulation (2, 13⇓–15). Ablation of OGT is embryonically lethal or developmentally limiting in animal models (10, 16, 17) with the noted exception of Caenorhabditis elegans. In C. elegans, the phenotypes of oga-1 and ogt-1 null mutants suggest the enzymes are involved in macronutrient storage and life span (18⇓–20), and subsequent studies revealed the presence of O-GlcNAc modification at over 800 promoters associated with transcriptional programs influencing carbohydrate metabolism, stress responses, and longevity (21).

The presence of OGT in polycomb group repressor complexes further implicates OGT in the regulation of myriad genes involved in tissue differentiation and spatial patterning during embryogenesis (22, 23). In cellular models of differentiation, modulation of OGT and OGA impacts the expression and activity of markers characteristic of tissue specification (24⇓⇓⇓–28). This has been observed in differentiating osteoblasts whereby inhibition of OGA enhanced the expression of osteocalcin, a marker indicative of progression to the later stages of osteoblastogenesis (28). The increased rate of differentiation in osteoblasts was attributed to the potentiation of the transcriptional activity of runt-related transcription factor 2 (RUNX2), a master regulator of osteogenic programs, by O-GlcNAc modification of RUNX2 (28). Accumulating evidence continues to establish the adult skeleton as a nutrient- and insulin-sensitive tissue that communicates with other organs via bone-specific endocrine signals (e.g. osteocalcin (OCN) and fibroblast growth factor (FGF23)) thereby coupling bone metabolism to whole-body nutrient and mineral homeostasis (29⇓⇓⇓–33). O-GlcNAc modification of the insulin receptor substrates, critical mediators of insulin/IGF-1 signaling, raises the possibility that O-GlcNAc cycling serves as a nutrient-responsive regulatory mechanism in bone tissue (34). It is evident from immunoblot analysis that many proteins are O-GlcNAc-modified in osteoblasts, and the extent of protein O-GlcNAc modification changes during osteoblast differentiation (28). The aim of this study was to identify O-GlcNAc-modified substrates of OGT in differentiating osteoblasts.

Advances in methodology to isolate O-GlcNAcylated peptides and the recent availability of multiple modes of peptide fragmentation during tandem mass spectrometry are revealing the ubiquitous nature of this modification (1, 2, 35, 36). The enrichment of O-GlcNAc-modified peptides from complex mixtures of digested proteins has been achieved by enzymatic labeling of the N-acetylglucosamine with chemically reactive moieties amenable to affinity purification, immunoprecipitation with pan-specific anti-O-GlcNAc antibodies, or by wheat germ agglutinin (WGA) lectin affinity chromatography (1, 2, 36⇓⇓⇓–40). The identification of O-GlcNAcylated peptides by conventional collision-induced dissociation (CID) tandem mass spectrometry can be complicated by the labile nature of the O-linkage, which upon fragmentation results in neutral loss of GlcNAc from the Ser/Thr prior to fragmentation of the peptide backbone (36, 41, 42). This impedes detection of O-GlcNAc-modified peptides by database search algorithms and obscures identification of the sites of modification. Peptide fragmentation by electron transfer dissociation (ETD) provides an alternative approach that leaves the fragile O-linkage intact enabling the direct detection of O-GlcNAc-modified peptides and more confident assignment of the sites of modification (43, 44). With the capability of multiple modes of fragmentation, the generation of N-acetylglucosamine (GlcNAc) oxonium ions or the neutral loss of GlcNAc from precursor ions during CID or higher energy collision-induced dissociation (HCD) (36) can trigger the acquisition of an ETD spectrum yielding complementary tandem mass spectra of the peptide. The development of algorithms for searching ETD spectra against protein databases (45), the incorporation of neutral loss information during searches of CID spectra (46), and the association of a score with the assignment of the site of modification (47) are further enabling this field. A recent study employing lectin weak affinity chromatography (LWAC) and CID/ETD permitted the identification of over 1,750 unique sites of O-GlcNAcylation on 2,434 synaptosome peptides (2).

In this study, we employed lectin chromatography to identify O-GlcNAc-modified proteins by ETD MS/MS in MC3T3E1 preosteoblasts cultured for 7 days under osteogenic conditions. Although many of the proteins observed in this study were previously reported to be O-GlcNAc-modified in other cell types, in some cases different sites of O-GlcNAc were elucidated. Of particular interest with respect to osteoblast function were sites of O-GlcNAc modification on the TFGβ-activated kinase 1/MAP3K7-binding protein 1 and 2 (TAB1/TAB2), regulatory subunits associated with the TFGβ-activated kinase 1 (TAK1) signaling node (48). Recent findings demonstrate that TAK1 signaling is essential to osteoblast differentiation and skeletal ossification, and it modulates RUNX2 activation and its association with the CREB-binding protein (CBP) (49). CBP, a histone acetyltransferase and transcriptional co-activator, is critical for osteoblast differentiation and skeletal mineralization. A new site of O-GlcNAc modification was observed in the C-terminal domain of CBP at Ser-2360, a known site of phosphorylation (53, 54). Also observed were sites of O-GlcNAcylation on nucleobindin 2 (precursor to the anorexic hormone, nesfatin-1) (55) and the Ser/Thr kinase WNK1 (56), which have roles in nutrient and electrolyte homeostasis, respectively.

EXPERIMENTAL PROCEDURES

Materials

Antibodies and providers were as follows: the pan-specific, monoclonal CTD 110.6 antibody specifically recognizing Ser/Thr β-O-linked N-acetylglucosamine (57) was from ThermoScientific (Rockford, IL); GAPDH (H-12), RUNX2 (M-70), α-tubulin (B-7), and HA peptide (F-7) antibodies were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA); CBP (D6C5), rabbit IgG (2729), and TAB2 (3744S) antibodies were from Cell Signaling (Danvers, MA); OGT antibody was from Sigma-Aldrich; OGA (MGEA5) antibody was from ProteinTech Group Inc. (Chicago, IL); anti-FLAG epitope tag (3×FLAG) was from Fisher; NUCB2 (nesfatin-1) antibody recognizing nucleobindin 2 residues 1–45 was from Phoenix Pharmaceuticals (Burlingame, CA). The O-GlcNAcase inhibitor O-(2-acetamido-2-deoxy-d-glucopyranosylidene)amino N-phenyl carbamate (PUGNAc) (58) was from Toronto Research Chemicals (Ontario, Canada), and Thiamet G, a more potent and selective inhibitor of OGA enzyme activity (59), was from Cayman Chemical (Ann Arbor, MI).

Osteoblast Cell Culture

Primary normal human osteoblasts (NHOst) (Lonza Inc., Walkersville, MD) and murine C57BL/6 calvarial MC3T3E1 preosteoblasts (subclone E4) (ATCC, Manassas, VA) were cultured in α-minimal essential medium (Cellgro-Mediatech, Manassas, VA) with 10% FBS and 1% penicillin/streptomycin. Cells were grown to subconfluency (90%) before inducing osteogenesis; NHOst cells were differentiated in the presence of 400 nm hydrocortisone and 7.5 mm β-glycerol phosphate (as optimized by the manufacturer), whereas MC3T3E1 cells were differentiated in the presence of 5 mm β-glycerol phosphate and 50 μg/ml ascorbic acid. Cells were incubated at 37°C in the presence of 5% CO2. Osteogenic medium was changed every other day for up to 21 days, during which time cells were harvested for mRNA transcript or protein analysis as described below or, alternatively, stained for alkaline phosphatase (ALP) activity. For ALP staining, the MC3T3E1 monolayer was washed twice with phosphate-buffered saline (PBS) and fixed for 10 min in 2% paraformaldehyde in PBS. The cells were then washed twice with PBS, twice more with 100 mm Tris-HCl, pH 9.4, 100 mm NaCl, 50 mm Mg MgCl2, 0.1% Tween 20 before incubating with BM Purple alkaline phosphatase substrate (Roche Applied Science) for 18 h. The reaction was terminated by washing cells three times with deionized water.

Quantitative Real Time PCR

Total RNA was extracted from cells using the RNeasy mini kit (Qiagen, Valencia, CA), and the concentration was determined by UV absorbance at 260 nm using a NanoDrop 2000 UV-visible spectrophotometer (ThermoScientific). Messenger RNA (1 μg; 260/280 nm ≥2.00) was reverse-transcribed with iScript cDNA synthesis reagents (Bio-Rad), and the resulting cDNAs were amplified with an iCycler iQ real time PCR detection system (Bio-Rad) in the presence of IQ SYBR Green Supermix (Bio-Rad) and oligonucleotide primers. Sequence-specific primers were as follows: OCN, forward 5′-GACAAAGCCTTCATGTCCAAG-3′ and reverse 5′-AAAGCCGAGCTGCCAGAGTTT-3′; OGT forward 5′-GACGCAACCAAACTTTGCAGT-3′ and reverse 5′-TCAAGGGTGACAGCCTTTTCA-3′; OGA forward 5′-TATGCTATTTCGCCAGGACTAGA-3′ and reverse 5′-GACCTGCACCCAAACTGAGA-3′ and GAPDH forward 5′-CAGCAAGGACACTGAGCAAG-3′ and reverse 5′-GGTCTGGGATGGAAATTGTG-3′. Resulting amplification products were quantified with iCycler iQ version 3.1 system software (Bio-Rad).

Preparation of Proteins for Immunoblot Analysis

Cells were washed twice with ice-cold PBS and lysed in 15 mm Tris-HCl, pH 7.4, 150 mm NaCl, 1 mm EDTA, 1% Nonidet P-40 amended with complete-mini EDTA-free protease inhibitor mixture (Sigma-Aldrich), phosphatase inhibitors sodium fluoride (10 mm), sodium orthovanadate (0.5 mm), sodium pyrophosphate (1 mm), and the β-N-acetylglucosaminidase inhibitor PUGNAc (50 μm). Insoluble material was removed by centrifugation at 8,000 × g for 10 min. Alternatively, crude nuclear and cytosolic fractions were prepared from cells using NE-PER extraction reagents (ThermoScientific) according to the manufacturer's instructions. GAPDH, RUNX2, and/or HisH1 served as endogenous, cytosolic, or nuclear fraction markers, respectively. Protein concentration was determined by the Bradford assay (ThermoScientific) for both total cell lysates and subcellular fractions.

For immunoblot analysis, proteins were resolved by SDS-PAGE, transferred to nitrocellulose membrane, and probed with protein- or modification-specific antibodies for 18 h at 4°C. Membranes were incubated with 1:20,000 secondary dye-conjugated goat anti-rabbit IRDye 680RD or goat anti-mouse 800CW IgG antibodies (Li-Cor; Lincoln, NE). Immunoblots employing the CTD 110.6 O-GlcNAc primary antibody were incubated with the secondary Alexa Fluor goat anti-mouse 680 IgM antibody (Invitrogen). Proteins were visualized with an Odyssey infrared imaging system (Li-Cor).

LWAC Enrichment of O-GlcNAc-modified Peptides

MC3T3E1 cells were cultured for 7 days under osteogenic conditions and harvested in 50 mm Tris-HCl, pH 7.4, 300 mm NaCl, 1% Nonidet P-40 amended with protease, phosphatase, and O-GlcNAcase inhibitors as described above. Protein was precipitated in acetone, resolubilized in 6 m guanidine hydrochloride, and diluted with 50 mm ammonium bicarbonate. Cysteines were reduced in 2 mm tris(2-carboxyethyl)phosphine hydrochloride at 55°C for 1 h and alkylated in 10 mm iodoacetamide at ambient temperature in the dark for 30 min. Protein was digested at a 1:100 concentration with sequencing-grade, modified trypsin (Promega, Madison WI) at 37°C for 40 h. Resulting peptides were centrifuged to remove any remaining insoluble material, acidified with formic acid, and desalted using tC18 Sep-Pak cartridges (Waters). Dried tryptic peptides were solubilized in 0.2 ml of column buffer (25 mm Tris, pH 7.8, 300 mm NaCl, 5 mm CaCl2·2H2O, 1 mm MgCl2·6H2O) and loaded onto a column containing agarose-coupled WGA, prepared as described previously (38, 39). A maximum of 2 mg of peptides was loaded onto the column. The flow rate was 100 μl/min, and eluting fractions were collected and screened for the presence of O-GlcNAc-modified peptides by LC-MS/MS and dot-blot immunoassay with the CTD110.6 O-GlcNAc antibody (supplemental Fig. 2). Positive fractions were combined, desalted, and dried under vacuum.

Separation and MS/MS Analysis of O-GlcNAc-modified Peptides

Peptides from combined fractions from a single LWAC enrichment were separated by C18 reversed phase nano-LC on a 75-μm × 20-cm capillary column packed in-house (YMC ODS-AQ 120Å S5; Waters) with a gradient of 5–60% B over 120 min (mobile phase A: 2% acetonitrile, 0.2% formic acid; mobile phase B: acetonitrile, 0.2% formic acid) at 200 nl/min using an Ultimate 3000 nanoflow system equipped with Chromeleon 6.8 software (Dionex, Sunnyvale, CA). To increase the yield, O-GlcNAc-positive fractions from three and then seven sequential LWAC enrichments were combined and separated by two-dimensional LC. The first dimension consisted of a step gradient of 0, 10, 20, 40, 50, 60 75, and 100 mm ammonium acetate (mobile phase A: 2% acetonitrile, 0.2% formic acid; mobile phase B: 500 mm ammonium acetate, pH 2.8) on a 0.5 × 50-mm BioBasic SCX KAPPA column (Fisher). Peptides eluted in each salt step were desalted in-line with an Acclaim PepMap 100 C18 μ-precolumn (0.3 × 5 mm, 5 μm, 100 Å; Dionex) and separated in the second dimension by C18 reversed phase nano-LC as described above.

Peptides were fragmented by alternating CID/ETD MS/MS or by ETD MS/MS only on the five most abundant ions in the full mass spectrum survey scan (m/z range, 400–2000) with an LTQ XL ion-trap mass spectrometer (ThermoFisher). The parameters used for ETD were as follows: 100 ms activation time; emission current, 130 μA; automatic gain control, 30,000; temperature, 170°C; supplemental activation was turned off; isolation width, 4 Da; reagent ion, fluoranthene. Dynamic exclusion was enabled with a repeat count of two, a repeat duration of 30 s, and exclusion duration of 180 s. In subsequent studies, an aliquot of the seven-enrichment pooled fraction was analyzed using an Orbitrap Elite (ThermoFisher). Peptides were separated by two-dimensional LC as described above, and full mass spectrum survey scans (m/z 400–2000) were acquired at a resolution of 60,000. The top 10 most abundant ions in each survey scan were fragmented by HCD using a collision energy of 35, and the product ions were detected in the Orbitrap. The presence of HexNAc oxonium ions (m/z 138.06, 186.08, and 204.1) within the top 30 product ions triggered the acquisition of an ETD fragmentation spectrum of the precursor ions in the Velos ion trap.

Data Analysis

Xcalibur RAW files were converted to peak list files in -mzXML format using ReAdW-3.5.1. CID/ETD data were searched separately using the Batch Tag Web feature of Protein Prospector 2 version 5.7.3 against the SwissProt database 2011.01.11 (Mus musculus, 16,338 entries). ETD spectra were searched with allowances for variable HexNAc modifications (+203.2 Da) on Ser/Thr (O-linkage) and Asn (N-linkage) residues, as well as oxidation on Met residues with an upper limit of four modifications per peptide. Carbamidomethylated cysteine was included as a static modification. CID data were searched using the same parameters allowing the neutral loss of HexNAc from the precursor and fragment ions. Trypsin was selected as the proteolytic enzyme with a maximum of two missed cleavages allowed. Precursor ion and fragment ion mass tolerances were set at 1 and 1.5 Da, respectively. Data obtained on the Orbitrap were converted to -mzXML format using a converter obtained from ProteoWIZARD (version 3.0.4171). HCD and ETD data were searched against the SwissProt database 2012.03.21 (M. musculus, 16,520 entries) using Prospector version 5.10.8 with 50 ppm precursor tolerance and 0.5-Da fragment tolerance. The minimum peptide score was 15, and the maximum peptide expectation value was 0.05. Tandem mass spectra corresponding to putative O-GlcNAc-modified peptides were manually confirmed by comparing experimental sequence ions to theoretical fragment ions generated by Protein Prospector. CID and HCD spectra of putative O-GlcNAc-modified peptides were examined for the presence of the expected neutral loss of HexNAc from the precursor ion and/or the generation of HexNAc oxonium ions.

Transient TAB2 or CBP Expression

For immunological characterization of TAB2 and CBP O-GlcNAc modification, HEK293 cells were transfected with human CMV promoter-driven N-terminal 3×FLAG-tagged TFGβ-activated kinase 1/MAP3K7-binding protein 2 (TAB2) (GeneCopoeia Inc., Rockville, MD) or mouse RSV promoter-driven C-terminal HA-tagged CBP (Invitrogen) in the presence of PolyFect transfection reagent (Qiagen). After 48 h, cells were washed twice and then cultured for 18 h in modified improved essential medium (Invitrogen) with 1% FBS, 1% penicillin, streptomycin/amphotericin B (HyClone, Logan, UT) in the absence or presence of PUGNAc (50–100 μm) or Thiamet G (20 μm).

Total cell lysates or nuclear and cytosolic fractions were prepared as described above. TAB2 was immunoprecipitated from 250 to 500 μg of total protein with 3×FLAG antibody-conjugated agarose beads (Sigma-Aldrich) at 4°C. CBP was immunoprecipitated from 0.5 to 1 mg of total protein in the presence of protein A-agarose beads (EMD Chemicals, Inc., San Diego) at 4°C. Mock immunoprecipitations were performed with normal rabbit IgG (0.5 μg/mg) antibody to control for nonspecific interactions. Beads were collected by centrifugation and washed extensively with 150–300 mm NaCl in 15 mm Tris HCl, pH 7.4. Immunoprecipitates were eluted with an equal bead volume of 2× XT gel loading sample buffer (Bio-Rad) with 10% β-mercaptoethanol at 100°C for 5 min. Gel-purified and trypsin-digested CBP was also analyzed by LC-MS/MS using HCD-triggered ETD on the Orbitrap Elite mass spectrometer as described above.

RESULTS

The MC3T3E1 (subclone E4) cells can be differentiated into osteoblasts in the presence of ascorbic acid and β-glycerol phosphate, resulting in increased expression of osteoblast-specific markers ALP and OCN, secretion of collagen matrix, and the formation of mineral nodules (60⇓–62). In this study, MC3T3E1 cells were cultured under osteogenic conditions for 0, 3, 7, 14, and 21 days, and the progression of osteoblast differentiation was confirmed by measuring OCN transcript expression and ALP activity (Fig. 1). The pattern of protein O-GlcNAc modification was assessed by immunoblot analysis using a pan-specific CTD 110.6 anti-O-GlcNAc antibody. Consistent with previous observations (28), the pattern and intensity of protein O-GlcNAcylation varied during the progression of osteogenesis with the highest overall levels of modification occurring between days 3 and 7 (Fig. 1A). To test whether this also occurs in primary osteoblasts, NHOst were differentiated, and the protein was probed for O-GlcNAc modification by immunoblot analysis. Similar to murine MC3T3E1 cells, a burst of elevated protein O-GlcNAcylation occurred during the first few days of differentiation in NHOst cells (supplemental Fig. 1). Changes in the extent of protein O-GlcNAc modification occurring concomitantly with increased expression OGT and OGA have been observed during the differentiation of mesenchymal stem cells toward myoblasts or chondrocytes (26, 27). During the time course of differentiation in MC3T3E1 cells the expression of OGT and OGA did not change (Fig. 1A). As this was the first analysis of OGT and OGA protein expression in osteoblasts, we also confirmed that OGT was localized preferentially in the nuclear compartment, and OGA was primarily localized in the cytosol (Fig. 1C). Inhibition of OGA with PUGNAc resulted in increased O-GlcNAc modification of nuclear and cytosolic proteins, and as observed previously in vivo, the expression of OGA increased in response to inhibition (63).

Fig. 1.
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Fig. 1.

Changes in global protein O-GlcNAc modification during differentiation of MC3T3E1 osteoblasts. MC3T3E1 cells were differentiated for up to 21 days under osteogenic conditions. A, Protein lysates (30 μg) were probed for protein O-GlcNAc modification and OGT, OGA, and α-tubulin as a loading control. Three biological replicates are shown. B, Elevated expression of osteoblast markers was confirmed by quantitative PCR analysis of OCN transcript (left panel) and staining for ALP activity (right panel). OCN transcript levels were normalized against GAPDH (mean ± S.E., n = 3). Significant differences between day 0 and any given time point were determined by Student's unpaired t test: p < 0.01 < (**), or p < 0.001 (***). C, Immunoblot analysis of cytosolic (C) and nuclear (N) fractions prepared from cells differentiated in the presence of osteogenic factors (5 mm β-glycerol phosphate, 50 μg/ml ascorbate) for 3 days with 10% FCS or 1% FCS in the absence or presence of the OGA inhibitor PUGNAc. GAPDH, histone H1 (HisH1), and RUNX2 served as controls for subcellular fractionation and loading.

To identify O-GlcNAc-modified substrates of OGT in differentiating osteoblasts, WGA LWAC was employed to enrich O-GlcNAc-modified peptides from MC3T3E1 cells cultured under osteogenic conditions for 7 days. Harvested protein was digested with trypsin, and the resulting peptides were separated by lectin chromatography on a 3-m WGA-conjugated agarose column, as described previously (38). Fractions were collected and screened for the presence of O-GlcNAc-modified peptides by dot-blot immunoassay with the CTD 110.6 antibody (supplemental Fig. 2) and by LC-MS/MS. Peptides containing N- or O-linked HexNAc monosaccharides were minimally retained on the column and eluted at the end of the main peak after the bulk of unmodified peptides. These fractions were combined and analyzed by tandem mass spectrometry using multiple complementary fragmentation approaches as follows: 1) alternating CID/ETD fragmentation; 2) ETD alone; 3) and on the Orbitrap, the acquisition of ETD spectra was triggered upon detection of HexNAc-derived oxonium ions following HCD. Of the putative O-GlcNAc-modified peptides detected, 23 could be confidently confirmed by manual inspection of the spectra (supplemental material). Nine novel sites of modification were assigned (Table I). Although the lectin chromatography enrichment approach was relatively simple, the majority of peptides identified in this study were unmodified. Recent improvements in the lectin chromatography approach are now yielding much more efficient enrichment of O-GlcNAc-modified peptides, including one of the largest O-GlcNAc-modified proteome datasets achieved to date (2).

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Table I Tandem mass spectrometric identification of O-linked HexNAc-modified peptides isolated from differentiating osteoblasts

Novel sites of modification are in boldface, and ambiguous sites of modification are underlined. An asterisk indicates a site of HexNAc modification either at or proximal to a known site of phosphorylation (± four amino acids) (see supplemental Table 2).

The specificity of OGT for target proteins is thought to be mediated by associated regulatory proteins (2, 64⇓–66). Although efforts to define a consensus motif for O-GlcNAc modification indicate a 10–25-fold enrichment of O-GlcNAcylated sites within a P-X-T(GlcNAc)-X-A and P-V-S(GlcNAc) sequence, respectively (1, 38), a strict consensus motif has not been identified. Examination of OGT substrates with three-dimensionally resolved structures reveals that modification occurs within flexible, solvent-accessible regions that may also be highly phosphorylated (2). Consistent with these findings, sites of O-GlcNAc modification identified in osteoblasts occurred in the vicinity of proline and valine residues, within stretches of multiple serines and threonines, and in close proximity to sites of phosphorylation (supplemental Table 2). In addition to the potential for cross-talk among modifications at specific residues, OGT may impact signaling through the modification of regulatory enzymes (1, 2, 67). O-GlcNAc modification was observed on CBP lysine acetyltransferase, MAST4 and WNK1 kinases, and UBAP2L, a protein involved in ubiquitin conjugation. We also observed O-GlcNAc modification of proteins involved in systemic nutrient homeostasis, including perilipin, a protein involved in lipid droplet formation, and NUCB2, the precursor to the anorexic hormone nesfatin-1. Many of the proteins identified in our screen are involved in transcriptional regulation or have been observed in the nucleus within other cell types. These include TAB2 (68), HCF1 (69), SBNO1 (70), NFRκB (71), CNOT4 (72), NUCB2 (73), PHF21A (74), NF1A (75), Nup214 (76), LPP (77, 78), and CBP (79). The nuclear localization of TAB2, CBP, and NUCB2, was confirmed in differentiated MC3T3E1 cells (Fig. 1D). With regard to osteoblastogenesis, TAK-1 and CBP are critical for skeletal development, and thus we focused further efforts on the TAK1-associated regulatory subunit TAB2 and CBP.

O-GlcNAc modification of TAB2 was observed at Thr-456. The CID MS/MS spectrum of the HexNAc-modified peptide, residues 453–465, of TAB2 exhibited the characteristic neutral loss of the N-acetylglucosamine moiety from the doubly (m/z 762.96) and triply (m/z 509.13) charged precursor ions and the presence of HexNAc oxonium ions at m/z 204.01, 168.08, and 186.07 (supplemental material). Dissociation of the peptide by ETD permitted localization of the site of O-GlcNAc modification at Thr-456 (Fig. 2). This peptide was observed previously in HeLa cells using an enzymatic labeling approach; however, the site could not be assigned (48). Thr-456 is proximal to several sites of phosphorylation at residues Ser-419, Ser-423, Ser-447, Thr-449, and Ser-450. In addition to modulating TAK1 signaling in the cytoplasm, TAB2 functions as a transcriptional co-repressor in association with HDAC3 and NCoR (68). Phosphorylation at Ser-419 and Ser-423, which lie within a nuclear export sequence, regulates trafficking of TAB2 from the nucleus. To determine whether O-GlcNAc modification impacted the subcellular localization of TAB2, the protein was transiently expressed in HEK293 cells grown in the presence or absence of OGA inhibitors and immunoprecipitated from nuclear and cytosolic fractions. Immunoblot analysis demonstrated that treatment with PUGNAc or Thiamet G increased the extent of TAB2 O-GlcNAcylation in the nucleus and cytoplasm; however, the subcellular localization was not significantly altered (Fig. 2). Immunoblotting with the CTD 110.6 O-GlcNAc antibody further validated the observed 203.2 Da mass shift on TAB2 as GlcNAc rather than GalNAc.

Fig. 2.
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Fig. 2.

O-GlcNAc modification of TGF-β-activated kinase 1/MAP3K7-binding protein 2 (TAB2), in differentiating MC3T3E1 osteoblasts. The ETD tandem mass spectrum of TAB2, residues 453–465, confirms a 203.2-Da mass shift at residue Thr-456 (top panel). The peaks labeled with asterisks correspond to unfragmented precursor ions that have undergone a neutral loss of m/z 203.2. Immunoblot analyses of TAB2 immunoprecipitated from nontransfected (NT) or 3×FLAG-TAB2-transfected HEK293 cells cultured in the presence of PUGNAc (50 μm) or Thiamet G (20 μm) further confirm the 203.2-Da mass shift is due to O-GlcNAc modification and reveal the presence of O-GlcNAc-modified TAB2 in both the nuclear and cytoplasmic compartments.

Novel sites of HexNAc modification were observed within the N and C termini of CBP, a highly modified lysine acetyltransferase that is auto-acetylated, phosphorylated, methylated, and sumoylated (53). ETD MS/MS analysis permitted the unambiguous identification of O-GlcNAc modification at Ser-2360, a known site of phosphorylation (Fig. 3) (54). O-GlcNAc modification of CBP was also observed at Ser-147 by CID MS/MS (supplemental material). Although this O-GlcNAcylated peptide, residues 135–156, was observed in brain and synaptosomal preparations, the exact site of modification had not been assigned (1, 2, 13). O-GlcNAc modification at Ser-147 was further confirmed in HEK293 cells by ETD MS/MS (supplemental Fig. 3). The extent of O-GlcNAc modification of CBP increased in the nuclear fractions upon inhibition of OGA, and the localization of protein did not appear to change with enhanced O-GlcNAc modification.

Fig. 3.
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Fig. 3.

O-GlcNAc modification of CBP in differentiating MC3T3E1 osteoblasts. The ETD tandem mass spectrum of HexNAc-modified CBP, residues 2344–2365, indicates O-GlcNAc modification at Ser-2360. Immunoblot analysis of CBP immunoprecipitated from cytosolic and nuclear fractions of HEK293 treated with Thiamet G (20 μm) indicates the presence of O-GlcNAc modification of CBP localized in the nucleus. Immunoprecipitation with IgG was performed as a control for nonspecific binding.

As noted previously, the WGA lectin chromatography approach yields N-linked HexNAc-modified peptides as well as O-linked HexNAc (39). N-Linked HexNAc modification was observed on sulfated glycoprotein 1 (80) and osteonectin/SPARC, one of the most abundant secreted glycoproteins within the bone extracellular matrix (supplemental Table 1) (81).

DISCUSSION

Multiple O-GlcNAc-modified proteins were identified in MC3T3E1 lysates by MS/MS, expanding upon the results of Kim et al. (28), which demonstrated an elevation in protein O-GlcNAc modification during osteoblast differentiation. Although findings from that study suggested that global O-GlcNAc levels were indiscriminately enhanced in MC3T3E1 across a 7-day time period, our expansion of this assay to 21 days revealed more dynamic patterns of O-GlcNAc modification with the progression of osteogenesis. This suggests that O-GlcNAc modulation of specific signaling components may be altered during different phases of osteoblast differentiation.

Accumulating evidence supports a role for OGT and OGA in the modulation of master transcriptional regulators involved in the differentiation of various lineages derived from mesenchymal stromal cells, such as adipocytes, myoblasts, chondrocytes, and osteoblasts. In adipocytes, elevated O-GlcNAcylation of adipogenic CCAAT/enhancer-binding protein β and peroxisome proliferator-activated receptor γ decreased the DNA binding and transcriptional activity, respectively, and hindered terminal adipocyte differentiation (24, 25). In C2C12 myoblasts, chronic inhibition of OGA selectively reduced expression of myogenic markers myogenin and MRF4 and blocked myotube formation (26). In ATDC5 chondrocytes, pharmacological inhibition of OGA increased the progression of chondrogenesis (59) and enhanced the expression and activity of matrix metalloprotease (MMP) 2 and 9 (27). In osteoblasts, Kim et al. (28) proposed the increased rate of PTH- and forskolin-stimulated osteoblast differentiation, which occurred with inhibition of OGA, resulted from enhanced transcriptional activity of RUNX2. In this study, we identified O-GlcNAc modification of multiple proteins potentially involved in regulating CREB- and RUNX2-mediated transcription, including CBP, TAB1, and TAB2.

O-GlcNAc modification events have been described on all major components of the TAK1-signaling node, including TAB1 (1, 2, 82), TAB2 (48), TAB3 (1, 2), and TAK1 (1); however, the functional impact of O-GlcNAc modification on TAK1-mediated signaling in osteoblasts has not been addressed. Signal transduction through the TAK1 complex is stimulated by IL-1β, TGFβ, and BMPs. In osteoblasts, signaling through TAK1 modulates transcription by enhancing the association of RUNX2 and CBP, thereby influencing osteoblast differentiation, mineralization, and skeletal development (49). In this study, we observed O-GlcNAc modification of TAB1 and TAB2 and demonstrated for the first time that TAB2 is localized in the nucleus of differentiating osteoblasts.

Two sites of O-GlcNAc modification were assigned on the transcriptional co-activator CBP a 300 kDa acetyltransferase which participates in CREB coactivation. Heterozygous mutations in CBP and its paralog, p300, are associated with Rubenstein-Taybi syndrome, a clinical conditions characterized by mental retardation, microcephaly, and craniofacial abnormalities. Mice which are haploinsufficient for CBP exhibit defects in craniofacial skeletal development and mineralization (51). In osteoblasts, CBP is thought to interact and regulate the transcriptional activity of RUNX2 and CREB and contribute to CREB-mediated expression of BMP2 (51). The activity and protein interactions of CBP are known to be regulated by post-translational modifications. For example, the phosphorylation of CBP at Ser-89 blocks the binding of CBP to transcription factors such as CREB (79). In addition to CBP, CREB and CRTC2 (79) are also O-GlcNAcylated, and OGT has been shown to co-localize with CREB (83) at unique promoter regions in mouse neurons (84). Although the O-GlcNAc modification of an epigenetic regulator such as CBP is consistent with a mechanism proposed for OGT-mediated transcriptional control in a broad sense, it also implicates O-GlcNAc modification in the regulation of the anabolic effects elicited by PTH (50), insulin/IGF-1 (51), BMPs (49, 52), and Wnts (52) in osteoblasts.

Finally, the role of protein glycosylation in osteoblast function is intriguing in the context of a new paradigm that suggests that bone metabolism can directly influence whole-body glucose homeostasis (29, 30, 32, 85, 86). It has been shown that biologically active (i.e. under-carboxylated) and circulating OCN enhances glucose tolerance and insulin sensitivity in mice by stimulating the production of insulin and adiponectin from pancreatic β-cells and adipocytes, respectively (29⇓–31). Furthermore, the expression of OCN in osteoblasts is dependent upon insulin signaling, suggesting that bone and energy metabolism can reinforce one another in the absence of other negative regulatory factors. Being intimately connected to glucose flux through the hexosamine biosynthetic pathway, it has been proposed that the reversible O-GlcNAc modification is poised to respond to shifts in glucose and/or nutrient status (87). As reported for other cell types, we observe an increase in global protein O-GlcNAc modification in osteoblasts cultured in 25 mm versus 5 mm glucose (data not shown). As such, O-GlcNAcylation may represent a contributing mechanism for the control of bone anabolism at the level of the osteoblast. Studies are currently underway to explore the regulation of OGT and OGA as it relates to osteoblast survival and differentiation in the context of bone formation, fracture healing, and alveolar bone loss in diabetic patients.

Acknowledgments

We acknowledge the support of the Proteomics Center and Mass Spectrometry Facility, a University Research Resource at Medical University of South Carolina, and we thank Jennifer Rutherford Bethard for maintaining instrumentation. We also thank Robert Chalkley (University of California San Francisco) for help with the LWAC protocol and using Protein Prospector. We thank Matt Rice for technical help.

R01 DE020925T32 DE017551 05National Institutes of Health

Footnotes

  • ↵* This work was supported, in whole or in part, by National Institutes of Health Grants R01 DE020925 (to L.E.B.), and T32 DE017551 05 (to A.K.N.) from the NIDCR and Grants S10 D010731 (to L.E.B.), DK 055524-11A2S1 (to M.S.), and P20 GM103542 (to S.C.W.) from NCRR.

  • ↵Embedded Image This article contains supplemental material.

  • ↵1 The abbreviations used are:

    O-GlcNAc
    O-linked N-acetylglucosamine
    LWAC
    lectin weak affinity chromatography
    ETD
    electron transfer dissociation
    CBP
    CREB-binding protein
    RUNX2
    runt-related transcription factor 2
    OGT
    O-GlcNAc transferase
    OGA
    O-GlcNAcase
    WGA
    wheat germ agglutinin
    ALP
    alkaline phosphatase
    OCN
    osteocalcin
    PTH
    parathyroid hormone
    IGF-1
    insulin-like growth factor 1
    BMP
    bone morphogenetic protein
    CREB
    cAMP-response element-binding protein
    HCD
    higher energy collision-induced dissociation
    PUGNAc
    O-(2-acetamido-2-deoxy-d-glucopyranosylidene)amino N-phenyl carbamate
    CTD
    C-terminal domain
    NHOst
    normal human osteoblast.

  • Received December 10, 2012.
  • Revision received February 21, 2013.
  • © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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Identification of O-Linked N-Acetylglucosamine (O-GlcNAc)-modified Osteoblast Proteins by Electron Transfer Dissociation Tandem Mass Spectrometry Reveals Proteins Critical for Bone Formation
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Identification of O-Linked N-Acetylglucosamine (O-GlcNAc)-modified Osteoblast Proteins by Electron Transfer Dissociation Tandem Mass Spectrometry Reveals Proteins Critical for Bone Formation
Alexis K. Nagel, Michael Schilling, Susana Comte-Walters, Mary N. Berkaw, Lauren E. Ball
Molecular & Cellular Proteomics April 1, 2013, First published on February 26, 2013, 12 (4) 945-955; DOI: 10.1074/mcp.M112.026633

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Identification of O-Linked N-Acetylglucosamine (O-GlcNAc)-modified Osteoblast Proteins by Electron Transfer Dissociation Tandem Mass Spectrometry Reveals Proteins Critical for Bone Formation
Alexis K. Nagel, Michael Schilling, Susana Comte-Walters, Mary N. Berkaw, Lauren E. Ball
Molecular & Cellular Proteomics April 1, 2013, First published on February 26, 2013, 12 (4) 945-955; DOI: 10.1074/mcp.M112.026633
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Molecular & Cellular Proteomics: 12 (4)
Molecular & Cellular Proteomics
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1 Apr 2013
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