Skip to main content
Molecular & Cellular Proteomics

Main menu

  • Home
  • Articles
    • Current Issue
    • Papers in Press
    • Reviews and Minireviews
    • Special Issues
    • Editorials
    • Archive
    • Letters to the Editor (eLetters)
  • Info for
    • Authors
      • Editorial Policies
      • How to Submit
      • Manuscript Contents & Organization
      • Data Reporting Requirements
      • Publication Charges
    • Reviewers
    • Librarians
    • Advertisers
    • Subscribers
  • Guidelines
    • Proteomic Identification
      • Checklist (PDF)
      • Instructions for Annotated Spectra
      • Tutorial (PDF)
    • Clinical Proteomics
      • Checklist (PDF)
    • Glycomic Identification
      • Checklist (PDF)
    • Targeted Proteomics
      • Checklist (PDF)
    • Data-Independent Acquisition
      • Checklist (PDF)
    • Frequently Asked Questions
  • About
    • Mission Statement and Scope
    • Editorial Policies
    • Editorial Board
    • MCP Lectureships
    • Permissions and Licensing
    • Partners
    • Alerts
    • Contact Us

Submit

  • Submit
  • Publications
    • ASBMB
    • Molecular & Cellular Proteomics
    • Journal of Biological Chemistry
    • Journal of Lipid Research

User menu

  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
  • Publications
    • ASBMB
    • Molecular & Cellular Proteomics
    • Journal of Biological Chemistry
    • Journal of Lipid Research
  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Molecular & Cellular Proteomics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Papers in Press
    • Reviews and Minireviews
    • Special Issues
    • Editorials
    • Archive
    • Letters to the Editor (eLetters)
  • Info for
    • Authors
      • Editorial Policies
      • How to Submit
      • Manuscript Contents & Organization
      • Data Reporting Requirements
      • Publication Charges
    • Reviewers
    • Librarians
    • Advertisers
    • Subscribers
  • Guidelines
    • Proteomic Identification
      • Checklist (PDF)
      • Instructions for Annotated Spectra
      • Tutorial (PDF)
    • Clinical Proteomics
      • Checklist (PDF)
    • Glycomic Identification
      • Checklist (PDF)
    • Targeted Proteomics
      • Checklist (PDF)
    • Data-Independent Acquisition
      • Checklist (PDF)
    • Frequently Asked Questions
  • About
    • Mission Statement and Scope
    • Editorial Policies
    • Editorial Board
    • MCP Lectureships
    • Permissions and Licensing
    • Partners
    • Alerts
    • Contact Us
  • Submit
Research

Metabolomics Coupled with Proteomics Advancing Drug Discovery toward More Agile Development of Targeted Combination Therapies

Xijun Wang, Aihua Zhang, Ping Wang, Hui Sun, Gelin Wu, Wenjun Sun, Haitao Lv, Guozheng Jiao, Hongying Xu, Ye Yuan, Lian Liu, Dixin Zou, Zeming Wu, Ying Han, Guangli Yan, Wei Dong, Fangfang Wu, Tianwei Dong, Yang Yu, Shuxiang Zhang, Xiuhong Wu, Xin Tong and Xiangcai Meng
Molecular & Cellular Proteomics May 1, 2013, First published on January 29, 2013, 12 (5) 1226-1238; https://doi.org/10.1074/mcp.M112.021683
Xijun Wang
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: xijunwangls@126.com
Aihua Zhang
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ping Wang
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hui Sun
¶Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gelin Wu
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wenjun Sun
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Haitao Lv
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Guozheng Jiao
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hongying Xu
‖Research Center of Chinese Medicine, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ye Yuan
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lian Liu
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dixin Zou
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zeming Wu
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ying Han
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Guangli Yan
‖Research Center of Chinese Medicine, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wei Dong
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fangfang Wu
¶Department of Pharmaceutical Analysis, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tianwei Dong
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yang Yu
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shuxiang Zhang
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiuhong Wu
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xin Tong
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiangcai Meng
From the ‡National TCM Key Lab of Serum Pharmacochemistry, Key Lab of Chinmedomics, Heilongjiang University of Chinese Medicine and Key Pharmacometabolomic Platform of Chinese Medicines, Heping Road 24, Harbin 150040, China;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Figures & Data

Figures

  • Additional Files
  • Fig. 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 1.

    Histology, immunohistochemistry, and TUNEL assay. A, representative pictures of liver histopathology (H&E staining), immunohistochemistry, and TUNEL assay of apoptotic hepatocytes after CCL4 with DGR treatment in rats (original magnification: ×200). B, quantitative evaluation of the density of apoptotic hepatocytes using TUNEL staining. C, quantitative immunohistochemistry evaluation of the density of Fas- and BCL-2 positive cells. The values are expressed as mean ± S.D. and were compared via analysis of variance. *p < 0.05 versus the control group; **p < 0.01 versus the control group; #p < 0.05 versus the HI group; ##p < 0.01 versus the HI group.

  • Fig. 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 2.

    Metabolomic multivariate analysis. A, Base peak intensity chromatograms of urine samples. B, Base peak intensity chromatograms of plasma samples. C, OPLS-DA score plot for the control and HI groups. (▴, control group; ■, model group). D, the PLS-DA loading plots for the control and HI groups. E, a combination plot of S-plot and VIP values. The red point graph is the VIP value plot, which represents the value of each metabolite. The farther from the origin, the higher the VIP value of the metabolites. F, chemical structure and mass fragment information for 2-((hexyloxy) carbonyloxy)benzoic acid.

  • Fig. 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 3.

    Loading plots of OPLS-DA model of potential biomarkers identified. A, urine. B, plasma.

  • Fig. 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 4.

    Merged network combining major signaling networks associated with the metabolites differentially expressed in the Ingenuity pathway analysis. A, HI group; B, D group; C, DG group; D, DGR group.

  • Fig. 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 5.

    DGR causes synergistic effects on trajectory analysis of HI rats. For each cluster, dotted circles indicate the subject closest to the average user profile within the group. A, trajectory analysis of PCA score plots for the HI rats after D treatment. B, trajectory analysis of PCA score plots for the HI rats after G treatment. C, trajectory analysis of PCA score plots for the HI rats after R treatment. ■C, control; ■M (pink), HI group; ■COC (red), DGR group; ■DG (green); ■DR (brown); ■GR (yellow); ■G (blue); ■D (brown); ■R (green).

  • Fig. 6.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 6.

    Two-dimensional gel electrophoresis representative proteomic maps. A, the control group. B, the HI group. C, the YCHT-treated group. D, the DGR-treated group. The protein spots that are indicated with numbers represent the differentially expressed proteins.

Additional Files

  • Figures
  • Supplemental Data

    Files in this Data Supplement:

    • Supporting Information - Supporting Information
    • Supporting tables - Supporting tables
PreviousNext
Back to top
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Molecular & Cellular Proteomics.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Metabolomics Coupled with Proteomics Advancing Drug Discovery toward More Agile Development of Targeted Combination Therapies
(Your Name) has sent you a message from Molecular & Cellular Proteomics
(Your Name) thought you would like to see the Molecular & Cellular Proteomics web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Metabolomics Coupled with Proteomics Advancing Drug Discovery toward More Agile Development of Targeted Combination Therapies
Xijun Wang, Aihua Zhang, Ping Wang, Hui Sun, Gelin Wu, Wenjun Sun, Haitao Lv, Guozheng Jiao, Hongying Xu, Ye Yuan, Lian Liu, Dixin Zou, Zeming Wu, Ying Han, Guangli Yan, Wei Dong, Fangfang Wu, Tianwei Dong, Yang Yu, Shuxiang Zhang, Xiuhong Wu, Xin Tong, Xiangcai Meng
Molecular & Cellular Proteomics May 1, 2013, First published on January 29, 2013, 12 (5) 1226-1238; DOI: 10.1074/mcp.M112.021683

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Request Permissions

Share
Metabolomics Coupled with Proteomics Advancing Drug Discovery toward More Agile Development of Targeted Combination Therapies
Xijun Wang, Aihua Zhang, Ping Wang, Hui Sun, Gelin Wu, Wenjun Sun, Haitao Lv, Guozheng Jiao, Hongying Xu, Ye Yuan, Lian Liu, Dixin Zou, Zeming Wu, Ying Han, Guangli Yan, Wei Dong, Fangfang Wu, Tianwei Dong, Yang Yu, Shuxiang Zhang, Xiuhong Wu, Xin Tong, Xiangcai Meng
Molecular & Cellular Proteomics May 1, 2013, First published on January 29, 2013, 12 (5) 1226-1238; DOI: 10.1074/mcp.M112.021683
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

In this issue

Molecular & Cellular Proteomics: 12 (5)
Molecular & Cellular Proteomics
Vol. 12, Issue 5
1 May 2013
  • Table of Contents
  • Table of Contents (PDF)
  • Cover (PDF)
  • About the Cover
  • Index by author
  • Ed Board (PDF)

View this article with LENS

Jump to section

  • Article
    • Abstract
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • Footnotes
    • REFERENCES
  • Figures & Data
  • eLetters
  • Info & Metrics
  • PDF

  • Follow MCP on Twitter
  • RSS feeds
  • Email

Articles

  • Current Issue
  • Papers in Press
  • Archive

For Authors

  • Submit a Manuscript
  • Info for Authors

Guidelines

  • Proteomic Identification
  • Clinical Proteomics
  • Glycomic Identification
  • Targeted Proteomics
  • Frequently Asked Questions

About MCP

  • About the Journal
  • Permissions and Licensing
  • Advertisers
  • Subscribers

ASBMB Publications

  • Molecular & Cellular Proteomics
  • Journal of Biological Chemistry
  • Journal of Lipid Research
  • ASBMB Today

© 2019 American Society for Biochemistry and Molecular Biology | Privacy Policy

MCP Print ISSN 1535-9476 Online ISSN 1535-9484

Powered by HighWire