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Molecular & Cellular Proteomics

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Streamlined Protocol for Deep Proteomic Profiling of FAC-sorted Cells and Its Application to Freshly Isolated Murine Immune Cells

View ORCID ProfileSamuel A. Myers  Correspondence email, Andrew Rhoads, Alexandra R. Cocco, Ryan Peckner, Adam L. Haber, Lawrence D. Schweitzer, Karsten Krug, D. R. Mani, Karl R. Clauser, Orit Rozenblatt-Rosen, Nir Hacohen, Aviv Regev and Steven A. Carr  Correspondence email
Molecular & Cellular Proteomics May 1, 2019, First published on February 21, 2019, 18 (5) 995-1009; https://doi.org/10.1074/mcp.RA118.001259
Samuel A. Myers
From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142; §Klarman Cell Observatory, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142;
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  • ORCID record for Samuel A. Myers
  • For correspondence: sammyers@broadinstitute.org
Andrew Rhoads
¶Division of Immunology, Department of Microbiology and Immunobiology, Harvard Medical School, and Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women's Hospital, Boston, Massachusetts;
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Alexandra R. Cocco
From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;
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Ryan Peckner
From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;
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Adam L. Haber
From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142; §Klarman Cell Observatory, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142;
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Lawrence D. Schweitzer
From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;
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Karsten Krug
From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;
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D. R. Mani
From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;
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Karl R. Clauser
From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;
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Orit Rozenblatt-Rosen
From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142; §Klarman Cell Observatory, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142;
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Nir Hacohen
From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142; ‖Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts 02215; **Center for Cancer Immunology, Massachusetts General Hospital, Boston, Massachusetts 02114;
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Aviv Regev
From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142; §Klarman Cell Observatory, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142; ‡‡Howard Hughes Medical Institute and Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02140
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Steven A. Carr
From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;
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  • For correspondence: scarr@broad.mit.edu
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Streamlined Protocol for Deep Proteomic Profiling of FAC-sorted Cells and Its Application to Freshly Isolated Murine Immune Cells
Samuel A. Myers, Andrew Rhoads, Alexandra R. Cocco, Ryan Peckner, Adam L. Haber, Lawrence D. Schweitzer, Karsten Krug, D. R. Mani, Karl R. Clauser, Orit Rozenblatt-Rosen, Nir Hacohen, Aviv Regev, Steven A. Carr
Molecular & Cellular Proteomics May 1, 2019, First published on February 21, 2019, 18 (5) 995-1009; DOI: 10.1074/mcp.RA118.001259

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Streamlined Protocol for Deep Proteomic Profiling of FAC-sorted Cells and Its Application to Freshly Isolated Murine Immune Cells
Samuel A. Myers, Andrew Rhoads, Alexandra R. Cocco, Ryan Peckner, Adam L. Haber, Lawrence D. Schweitzer, Karsten Krug, D. R. Mani, Karl R. Clauser, Orit Rozenblatt-Rosen, Nir Hacohen, Aviv Regev, Steven A. Carr
Molecular & Cellular Proteomics May 1, 2019, First published on February 21, 2019, 18 (5) 995-1009; DOI: 10.1074/mcp.RA118.001259
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Molecular & Cellular Proteomics: 18 (5)
Molecular & Cellular Proteomics
Vol. 18, Issue 5
1 May 2019
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