Streamlined Protocol for Deep Proteomic Profiling of FAC-sorted Cells and Its Application to Freshly Isolated Murine Immune Cells

Samuel A. Myers; Andrew Rhoads; Alexandra R. Cocco; Ryan Peckner; Adam L. Haber; Lawrence D. Schweitzer; Karsten Krug; D. R. Mani; Karl R. Clauser; Orit Rozenblatt-Rosen; Nir Hacohen; Aviv Regev; Steven A. Carr

doi:10.1074/mcp.RA118.001259

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Streamlined Protocol for Deep Proteomic Profiling of FAC-sorted Cells and Its Application to Freshly Isolated Murine Immune Cells

View ORCID ProfileSamuel A. Myers Correspondence email, Andrew Rhoads, Alexandra R. Cocco, Ryan Peckner, Adam L. Haber, Lawrence D. Schweitzer, Karsten Krug, D. R. Mani, Karl R. Clauser, Orit Rozenblatt-Rosen, Nir Hacohen, Aviv Regev and Steven A. Carr Correspondence email

Molecular & Cellular Proteomics May 1, 2019, First published on February 21, 2019, 18 (5) 995-1009; https://doi.org/10.1074/mcp.RA118.001259

Samuel A. Myers

From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142; §Klarman Cell Observatory, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142;

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ORCID record for Samuel A. Myers
For correspondence: sammyers@broadinstitute.org

Andrew Rhoads

¶Division of Immunology, Department of Microbiology and Immunobiology, Harvard Medical School, and Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women's Hospital, Boston, Massachusetts;

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Alexandra R. Cocco

From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;

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Ryan Peckner

From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;

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Adam L. Haber

From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142; §Klarman Cell Observatory, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142;

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Lawrence D. Schweitzer

From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;

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Karsten Krug

From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;

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D. R. Mani

From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;

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Karl R. Clauser

From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;

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Orit Rozenblatt-Rosen

From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142; §Klarman Cell Observatory, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142;

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Nir Hacohen

From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142; ‖Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, Massachusetts 02215; **Center for Cancer Immunology, Massachusetts General Hospital, Boston, Massachusetts 02114;

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Aviv Regev

From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142; §Klarman Cell Observatory, The Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142; ‡‡Howard Hughes Medical Institute and Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02140

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Steven A. Carr

From the ‡The Broad Institute or MIT and Harvard, Cambridge, Massachusetts 02142;

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For correspondence: scarr@broad.mit.edu

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Streamlined Protocol for Deep Proteomic Profiling of FAC-sorted Cells and Its Application to Freshly Isolated Murine Immune Cells

Samuel A. Myers, Andrew Rhoads, Alexandra R. Cocco, Ryan Peckner, Adam L. Haber, Lawrence D. Schweitzer, Karsten Krug, D. R. Mani, Karl R. Clauser, Orit Rozenblatt-Rosen, Nir Hacohen, Aviv Regev, Steven A. Carr

Molecular & Cellular Proteomics May 1, 2019, First published on February 21, 2019, 18 (5) 995-1009; DOI: 10.1074/mcp.RA118.001259