Skip to main content
Molecular & Cellular Proteomics

Main menu

  • Home
  • Articles
    • Current Issue
    • Papers in Press
    • Reviews and Minireviews
    • Special Issues
    • Editorials
    • Archive
    • Letters to the Editor (eLetters)
  • Info for
    • Authors
      • Editorial Policies
      • How to Submit
      • Manuscript Contents & Organization
      • Data Reporting Requirements
      • Publication Charges
    • Reviewers
    • Librarians
    • Advertisers
    • Subscribers
  • Guidelines
    • Proteomic Identification
      • Checklist (PDF)
      • Instructions for Annotated Spectra
      • Tutorial (PDF)
    • Clinical Proteomics
      • Checklist (PDF)
    • Glycomic Identification
      • Checklist (PDF)
    • Targeted Proteomics
      • Checklist (PDF)
    • Data-Independent Acquisition
      • Checklist (PDF)
    • Frequently Asked Questions
  • About
    • Mission Statement and Scope
    • Editorial Policies
    • Editorial Board
    • MCP Lectureships
    • Permissions and Licensing
    • Partners
    • Alerts
    • Contact Us

Submit

  • Submit
  • Publications
    • ASBMB
    • Molecular & Cellular Proteomics
    • Journal of Biological Chemistry
    • Journal of Lipid Research

User menu

  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
  • Publications
    • ASBMB
    • Molecular & Cellular Proteomics
    • Journal of Biological Chemistry
    • Journal of Lipid Research
  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart
Molecular & Cellular Proteomics

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Papers in Press
    • Reviews and Minireviews
    • Special Issues
    • Editorials
    • Archive
    • Letters to the Editor (eLetters)
  • Info for
    • Authors
      • Editorial Policies
      • How to Submit
      • Manuscript Contents & Organization
      • Data Reporting Requirements
      • Publication Charges
    • Reviewers
    • Librarians
    • Advertisers
    • Subscribers
  • Guidelines
    • Proteomic Identification
      • Checklist (PDF)
      • Instructions for Annotated Spectra
      • Tutorial (PDF)
    • Clinical Proteomics
      • Checklist (PDF)
    • Glycomic Identification
      • Checklist (PDF)
    • Targeted Proteomics
      • Checklist (PDF)
    • Data-Independent Acquisition
      • Checklist (PDF)
    • Frequently Asked Questions
  • About
    • Mission Statement and Scope
    • Editorial Policies
    • Editorial Board
    • MCP Lectureships
    • Permissions and Licensing
    • Partners
    • Alerts
    • Contact Us
  • Submit
Research

Excretory/Secretory Proteome of the Adult Developmental Stage of Human Blood Fluke, Schistosoma japonicum

Feng Liu, Shu-Jian Cui, Wei Hu, Zheng Feng, Zhi-Qin Wang and Ze-Guang Han
Molecular & Cellular Proteomics June 1, 2009, First published on March 18, 2009, 8 (6) 1236-1251; https://doi.org/10.1074/mcp.M800538-MCP200
Feng Liu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shu-Jian Cui
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wei Hu
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zheng Feng
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhi-Qin Wang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ze-Guang Han
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: hanzg@chgc.sh.cn
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Schistosomes are the causative agents of schistosomiasis, one of the most prevalent and serious of the parasitic diseases that currently infects ∼200 million people worldwide. Schistosome excretory/secretory (ES) proteins have been shown to play important roles in modulating mammalian host immune systems. In our current study, we performed a global proteomics identification of the ES proteins from adult worms of Schistosoma japonicum, one of the three major schistosome species. Our results unambiguously identified 101 proteins, including 53 putatively secreted proteins. By quantitative analysis, we revealed fatty acid-binding protein as a major constituent of the in vitro ES proteome. Strikingly the heat shock proteins HSP70s, HSP90, and HSP97 constituted the largest protein family in the ES proteome, implying a central role for these proteins in immunomodulation in the host-parasite relationship. Other important S. japonicum ES proteins included actins, 14-3-3, aminopeptidase, enolase, and glyceraldehyde-3-phosphate dehydrogenase, some of which have been considered as viable vaccine candidates and therapeutic targets. A comparison with previous studies suggests that 48.5% of S. japonicum ES proteins are common to other parasite ES products, indicating that the molecular mechanisms involved in evading the host immune response may be conserved across different parasites. Interestingly seven host proteins, including antimicrobial protein CAP18, immunoglobulins, and a complement component, were identified among in vitro S. japonicum ES products likely originating from the schistosome tegument or gut, indicating that host innate and acquired immune systems could defend against schistosome invasion. Our present study represents the first attempt at profiling S. japonicum ES proteins, provides an insight into host-parasite interactions, and establishes a resource for the development of diagnostic agents and vaccines for the control of schistosomiasis.

Footnotes

  • ↵* This work was supported by Chinese National Key Program on Basic Research (973) Grants 2007CB513100 and 2006CB708510, Chinese High-Tech Research and Development Program (863) Grants 2006AA02Z318 and 2007AA02Z153, the National Foundation for Excellence Doctoral Project, Shanghai Commission for Science and Technology Grants 06JC14059 and 05DZ22201, the China Postdoctoral Science Foundation, and Shanghai Rising-Star Program Grant 07QA14043.

  • ↵Embedded Image The on-line version of this article (available at http://www.mcponline.org) contains supplemental material.

  • ↵2 M. Berriman, unpublished data.

  • ↵3 Z. Chen, unpublished data.

  • ↵4 S.-Y. Wang, unpublished data.

  • ↵1 The abbreviations used are:

    ES
    excretory/secretory
    CAP
    cytoskeleton-associated protein
    DB
    database
    ESP
    ES product
    EST
    expressed sequence tag
    FABP
    fatty acid-binding protein
    FPR
    false-positive rate
    GAPDH
    glyceraldehyde-3-phosphate dehydrogenase
    HSP
    heat shock protein
    Ig
    immunoglobulin
    nano-LC-MS/MS
    nanoscale capillary LC-MS/MS
    NC
    non-classical
    RP
    reverse-phase
    SC
    spectral count
    SCX
    strong cation exchange
    SOD
    superoxide dismutase
    SP
    signal peptide
    TPX
    thioredoxin peroxidase
    WCL
    whole cell lysis
    BLAST
    basic local alignment search tool
    Mowse
    molecular weight search
    Sj
    S. japonicum
    Sm
    S. mansoni
    MSD
    mass selective detector
    XCT
    X-ray computed tomography.

    • Received November 24, 2008.
    • Accepted March 11, 2009.
  • © 2009 by The American Society for Biochemistry and Molecular Biology, Inc.
View Full Text
PreviousNext
Back to top
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on Molecular & Cellular Proteomics.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Excretory/Secretory Proteome of the Adult Developmental Stage of Human Blood Fluke, Schistosoma japonicum
(Your Name) has sent you a message from Molecular & Cellular Proteomics
(Your Name) thought you would like to see the Molecular & Cellular Proteomics web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Excretory/Secretory Proteome of the Adult Developmental Stage of Human Blood Fluke, Schistosoma japonicum
Feng Liu, Shu-Jian Cui, Wei Hu, Zheng Feng, Zhi-Qin Wang, Ze-Guang Han
Molecular & Cellular Proteomics June 1, 2009, First published on March 18, 2009, 8 (6) 1236-1251; DOI: 10.1074/mcp.M800538-MCP200

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Request Permissions

Share
Excretory/Secretory Proteome of the Adult Developmental Stage of Human Blood Fluke, Schistosoma japonicum
Feng Liu, Shu-Jian Cui, Wei Hu, Zheng Feng, Zhi-Qin Wang, Ze-Guang Han
Molecular & Cellular Proteomics June 1, 2009, First published on March 18, 2009, 8 (6) 1236-1251; DOI: 10.1074/mcp.M800538-MCP200
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

In this issue

Molecular & Cellular Proteomics: 8 (6)
Molecular & Cellular Proteomics
Vol. 8, Issue 6
1 Jun 2009
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Back Matter (PDF)
  • Ed Board (PDF)
  • Front Matter (PDF)

View this article with LENS

Jump to section

  • Article
    • Abstract
    • EXPERIMENTAL PROCEDURES
    • RESULTS
    • DISCUSSION
    • Acknowledgments
    • Footnotes
    • REFERENCES
  • Figures & Data
  • eLetters
  • Info & Metrics
  • PDF

  • Follow MCP on Twitter
  • RSS feeds
  • Email

Articles

  • Current Issue
  • Papers in Press
  • Archive

For Authors

  • Submit a Manuscript
  • Info for Authors

Guidelines

  • Proteomic Identification
  • Clinical Proteomics
  • Glycomic Identification
  • Targeted Proteomics
  • Frequently Asked Questions

About MCP

  • About the Journal
  • Permissions and Licensing
  • Advertisers
  • Subscribers

ASBMB Publications

  • Molecular & Cellular Proteomics
  • Journal of Biological Chemistry
  • Journal of Lipid Research
  • ASBMB Today

© 2019 American Society for Biochemistry and Molecular Biology | Privacy Policy

MCP Print ISSN 1535-9476 Online ISSN 1535-9484

Powered by HighWire