- Almost three decades have elapsed since O’Farrell and Klose independently introduced high-resolution two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)—often referred to as gel-based proteomics—as a core technology for analyzing the protein composition of cells, tissue, or bio fluids. Since then, many technological developments have taken place leading to improvements in the procedures for detecting, quantitating, comparing, characterizing, and storing the wealth of information generated by this method.
- The sequencing of the human and other genomes is only the beginning of the quest to understand the functionality of cells, tissues, and organs in health and disease. Together with progress in bioinformatics, this development has paved the way to the revolution in biology and medicine that we are experiencing today. We are steadily moving from the study of single molecules to the analysis of complex biological systems, and the current explosion in the number of technologies available within proteomics and functional genomics promises to have a major impact on the way diseases will be diagnosed, treated, and managed in the near future.