PT - JOURNAL ARTICLE AU - Schwenk, Jochen M. AU - Igel, Ulrika AU - Neiman, Maja AU - Langen, Hanno AU - Becker, Charlotte AU - Bjartell, Anders AU - Ponten, Fredrik AU - Wiklund, Fredrik AU - Grönberg, Henrik AU - Nilsson, Peter AU - Uhlen, Mathias TI - Toward Next Generation Plasma Profiling via Heat-induced Epitope Retrieval and Array-based Assays AID - 10.1074/mcp.M110.001560 DP - 2010 Nov 01 TA - Molecular & Cellular Proteomics PG - 2497--2507 VI - 9 IP - 11 4099 - http://www.mcponline.org/content/9/11/2497.short 4100 - http://www.mcponline.org/content/9/11/2497.full SO - Mol Cell Proteomics2010 Nov 01; 9 AB - There is a need for high throughput methods for screening patient samples in the quest for potential biomarkers for diagnostics and patient care. Here, we used a combination of undirected target selection, antibody suspension bead arrays, and heat-induced epitope retrieval to allow for protein profiling of human plasma in a novel and systematic manner. Several antibodies were found to reveal altered protein profiles upon epitope retrieval at elevated temperatures with limits of detection improving into lower ng/ml ranges. In a study based on prostate cancer patients, several proteins with differential profiles were discovered and subsequently validated in an independent cohort. For one of the potential biomarkers, the human carnosine dipeptidase 1 protein (CNDP1), the differences were determined to be related to the glycosylation status of the targeted protein. The study shows a path of pursuit for large scale screening of biobank repositories in a flexible and proteome-wide fashion by utilizing heat-induced epitope retrieval and using an antibody suspension bead array format.