RT Journal Article SR Electronic T1 Comparative Performance of Four Methods for High-throughput Glycosylation Analysis of Immunoglobulin G in Genetic and Epidemiological Research JF Molecular & Cellular Proteomics JO Mol Cell Proteomics FD American Society for Biochemistry and Molecular Biology SP 1598 OP 1610 DO 10.1074/mcp.M113.037465 VO 13 IS 6 A1 Huffman, Jennifer E. A1 Pučić-Baković, Maja A1 Klarić, Lucija A1 Hennig, René A1 Selman, Maurice H. J. A1 Vučković, Frano A1 Novokmet, Mislav A1 Krištić, Jasminka A1 Borowiak, Matthias A1 Muth, Thilo A1 Polašek, Ozren A1 Razdorov, Genadij A1 Gornik, Olga A1 Plomp, Rosina A1 Theodoratou, Evropi A1 Wright, Alan F. A1 Rudan, Igor A1 Hayward, Caroline A1 Campbell, Harry A1 Deelder, André M. A1 Reichl, Udo A1 Aulchenko, Yurii S. A1 Rapp, Erdmann A1 Wuhrer, Manfred A1 Lauc, Gordan YR 2014 UL http://www.mcponline.org/content/13/6/1598.abstract AB The biological and clinical relevance of glycosylation is becoming increasingly recognized, leading to a growing interest in large-scale clinical and population-based studies. In the past few years, several methods for high-throughput analysis of glycans have been developed, but thorough validation and standardization of these methods is required before significant resources are invested in large-scale studies. In this study, we compared liquid chromatography, capillary gel electrophoresis, and two MS methods for quantitative profiling of N-glycosylation of IgG in the same data set of 1201 individuals. To evaluate the accuracy of the four methods we then performed analysis of association with genetic polymorphisms and age. Chromatographic methods with either fluorescent or MS-detection yielded slightly stronger associations than MS-only and multiplexed capillary gel electrophoresis, but at the expense of lower levels of throughput. Advantages and disadvantages of each method were identified, which should inform the selection of the most appropriate method in future studies.