RT Journal Article
SR Electronic
T1 Comparative Performance of Four Methods for High-throughput Glycosylation Analysis of Immunoglobulin G in Genetic and Epidemiological Research
JF Molecular & Cellular Proteomics
JO Mol Cell Proteomics
FD American Society for Biochemistry and Molecular Biology
SP 1598
OP 1610
DO 10.1074/mcp.M113.037465
VO 13
IS 6
A1 Huffman, Jennifer E.
A1 Pučić-Baković, Maja
A1 Klarić, Lucija
A1 Hennig, René
A1 Selman, Maurice H. J.
A1 Vučković, Frano
A1 Novokmet, Mislav
A1 Krištić, Jasminka
A1 Borowiak, Matthias
A1 Muth, Thilo
A1 Polašek, Ozren
A1 Razdorov, Genadij
A1 Gornik, Olga
A1 Plomp, Rosina
A1 Theodoratou, Evropi
A1 Wright, Alan F.
A1 Rudan, Igor
A1 Hayward, Caroline
A1 Campbell, Harry
A1 Deelder, André M.
A1 Reichl, Udo
A1 Aulchenko, Yurii S.
A1 Rapp, Erdmann
A1 Wuhrer, Manfred
A1 Lauc, Gordan
YR 2014
UL http://www.mcponline.org/content/13/6/1598.abstract
AB The biological and clinical relevance of glycosylation is becoming increasingly recognized, leading to a growing interest in large-scale clinical and population-based studies. In the past few years, several methods for high-throughput analysis of glycans have been developed, but thorough validation and standardization of these methods is required before significant resources are invested in large-scale studies. In this study, we compared liquid chromatography, capillary gel electrophoresis, and two MS methods for quantitative profiling of N-glycosylation of IgG in the same data set of 1201 individuals. To evaluate the accuracy of the four methods we then performed analysis of association with genetic polymorphisms and age. Chromatographic methods with either fluorescent or MS-detection yielded slightly stronger associations than MS-only and multiplexed capillary gel electrophoresis, but at the expense of lower levels of throughput. Advantages and disadvantages of each method were identified, which should inform the selection of the most appropriate method in future studies.