PT  - JOURNAL ARTICLE
AU  - Hsu, Chia-Wei
AU  - Chang, Kai-Ping
AU  - Huang, Yenlin
AU  - Liu, Hao-Ping
AU  - Hsueh, Pei-Chun
AU  - Gu, Po-Wen
AU  - Yen, Wei-Chen
AU  - Wu, Chih-Ching
TI  - Proteomic profiling of paired interstitial fluids reveals dysregulated pathways and salivary NID1 as a biomarker of oral cavity squamous cell carcinoma
AID  - 10.1074/mcp.RA119.001654
DP  - 2019 Jan 01
TA  - Molecular & Cellular Proteomics
PG  - mcp.RA119.001654
4099  - http://www.mcponline.org/content/early/2019/07/17/mcp.RA119.001654.short
4100  - http://www.mcponline.org/content/early/2019/07/17/mcp.RA119.001654.full
AB  - Patients with oral cavity squamous cell carcinoma (OSCC) are frequently first diagnosed at an advanced stage, leading to poor prognosis and high mortality rates. Early detection of OSCC using body fluid-accessible biomarkers may improve the prognosis and survival rate of OSCC patients. As tumor interstitial fluid is a proximal fluid enriched with cancer-related proteins, it is a useful reservoir suitable for the discovery of cancer biomarkers and dysregulated biological pathways in tumor microenvironments. Thus, paired interstitial fluids of tumor (TIF) and adjacent noncancerous (NIF) tissues from 10 OSCC patients were harvested and analyzed using one-dimensional gel electrophoresis coupled with liquid chromatography-tandem mass spectrometry (GeLC-MS/MS). Using label-free spectral counting-based quantification, 113 proteins were found to be upregulated in the TIFs compared to the NIFs. The gene set enrichment analysis (GSEA) revealed that the differentially expressed TIF proteins were highly associated with aminoacyl tRNA biosynthesis pathway. The elevated levels of 4 proteins (IARS, KARS, WARS, and YARS) involved in the aminoacyl tRNA biosynthesis were verified in the OSCC tissues with immunohistochemistry (IHC). In addition, nidogen-1 (NID1) was selected for verification as an OSCC biomarker. Salivary level of NID1 in OSCC patients (n = 48) was significantly higher than that in the healthy individuals (n = 51) and subjects with oral potentially malignant disorder (OPMD; n = 53). IHC analysis showed that NID1 level in OSCC tissues was increased compared to adjacent noncancerous epithelium (n =222). Importantly, the elevated NID1 level was correlated with the advanced stages of OSCC, as well as the poor survival of OSCC patients. Collectively, the results suggested that TIF analysis facilitates understanding of the OSCC microenvironment and that salivary NID1 may be a useful biomarker for OSCC.