Table I

Overview of phosphorylated peptide sequences identified on well characterized synaptic proteins

Seven sequences were identified on glutamate receptor NMDA, several of which are not yet annotated in the databases Phosphosite (www.phosphosite.org), Phosphobase (www.cbs.dtu.dk/databases/PhosphoBase/), or Human Protein Reference Database (www.hprd.org); 10 sequences were identified on PKC. Many other characteristic PSD proteins such as CaMKII, PSD-95, or DAP possess multiple uncharacterized sites.

ProteinAccessionPhosphopeptideResiduePosition
CaMKIIβ KCC2B_MOUSE ESSDSTNTTIEDEDAKSera397
CaMKIIβ KCC2B_MOUSE NSSAITSPKSer367
CaMKIIβ KCC2B_MOUSE QETVECLKKThr287
CaMKIIγ KCC2G_MOUSE QETVECLRThr287
CaMKK1 KKCC1_MOUSE SFGNPFEPQARSer458
CaMKK1 KKCC1_MOUSE SMSAPGSLLMKSera475
CaMKK2 KKCC2_MOUSE SFGNPFEGSRSera495
mAChR2 ACM2_MOUSE EPVANQDPVSPSLVQGRSer231
mAChR2 ACM2_MOUSE EPVANQDPVSPSLVQGRSer233
PSD-93 DLG2_MOUSE AISLEGEPRSer414
PSD-93 DLG2_MOUSE QPSVTLQRSera406
PSD-95 DLG4_MOUSE EQLMNSSLGSGTASLRSer415
PSD-95 DLG4_MOUSE EQLMNSSLGSGTASLRSer418
PSD-95 DLG4_MOUSE NTYDVVYLKTyr240
DAP-1 DLGP1_MOUSE SLDSLDPAGLLTSPKThr436
DAP-1 DLGP1_MOUSE GCSQDDECVSLRSer509
DAP-1 DLGP1_MOUSE ATQPSLTELTTLKSera389
DAP-1 DLGP1_MOUSE AVSEVSINRSera421
DAP-1 DLGP1_MOUSE GCSQDDECVSLRSer516
DAP-2 DLGP2_MOUSE ADSIEIYIPEAQTRSer1047
DAP-2 DLGP2_MOUSE SDVETATDSDTESRSera745
NR1 NMDZ1_MOUSE AITSTLASSFKSer890
NR2A NMDE1_MOUSE QDSLNQNPVSQRSera1025
NR2A NMDE1_MOUSE GSLIVDMVSDKSera929
NR2A NMDE1_MOUSE KMPSIESDVSer1459
NR2A NMDE1_MOUSE SPDFNLTGSQSNMLKThra888
NR2B NMDE2_MOUSE NMANLSGVNGSPQSALDFIRSera917
NR2B NMDE2_MOUSE QHSYDTFVDLQKSer1303
mGluR7 MGR7_MOUSE TELCENVDPNSPAAKSera900
PP2Bα PP2BA_MOUSE ITSFEEAKThra468
PKCα KPCA_MOUSE VISPSEDRSera318
PKCβ KPCB_MOUSE HPPVLTPPDQEVIRThr640
PKCε KPCE_MOUSE LAAGAESPQPASGNSPSEDDRSera337
PKCε KPCE_MOUSE SAPTSPCDQELKSera350
PKCγ KPCG_MOUSE TFCGTPDYIAPEIIAYQPYGKThra514
PKCγ KPCG_MOUSE MGPSSSPIPSPSPSPTDSKSera322
PKCγ KPCG_MOUSE SPTSPVPVPVMSera687
PKCγ KPCG_MOUSE TFCGTPDYIAPEIIAYQPYGKThra518
PKCγ KPCG_MOUSE MGPSSSPIPSPSPSPTDSKSera330
PKCγ KPCG_MOUSE AAPALTPPDRThr655
  • a A total of 21 phosphorylation sites not annotated at the time of analysis were identified.