Table II

Previously uncharacterized peptides from mouse brain tissue

All the peptides were identified using this new identification approach. The peptides in the table are identified from the sample in the second dataset or from similar samples. Mass is theoretical calculated in Da. See the supplemental data for a more detailed table and tandem mass spectra for all the peptides in the table. Residues in bold signify amino acids that are not part of the final (detected) sequence; * signifies a non-basic amino acid (not Lys or Arg).

UniProt accession no.Precursor nameSequenceTheoretical mass
Da
O70176 Pituitary adenylate cyclase-activating polypeptideAR↓GAGENLGGSAVDDPAPLT↓KR1639.77
P01193 Corticotropin-lipotropinPR↓KYVMGHF↓R**R880.43
P01193 Corticotropin-lipotropinKR↓ELEGERPLGLEQV↓LE1467.76
P01193 Corticotropin-lipotropinEE↓AVWGDGSPEPSPRE-amide↓GKR1481.69
P01193 Corticotropin-lipotropinEA↓VWGDGSPEPSPRE-amide↓GKR1410.65
P12961 Neuroendocrine protein 7B2KK↓acetyl-LLYEKMKGGQ↓RR1207.63
P12961 Neuroendocrine protein 7B2KK↓LLYEKMKGGQ↓RR1165.62
P16014 Secretogranin-1KR↓YPQSKWQEQE↓KN1321.6
P16014 Secretogranin-1RPR↓SEESQEREY↓KR1155.47
P16014 Secretogranin-1R**R↓DPADASGTRWASS↓RE1319.57
P26339 Chromogranin AKR↓LEGEDDPDRSMKLSF↓R*R1737.79
P26339 Chromogranin AKR↓LEGEDDPDRSM↓K***R1262.51
P35455 Vasopressin neurophysinVQ↓LAGTRESVDSAKPR↓VY1485.79
P35455 Vasopressin neurophysinR**R↓AREPSNATQLDGPA↓R****R1425.68
P35455 Vasopressin neurophysinTR↓ESVDSAKPRVY1249.63
P41539 Protachykinin 1KR↓DADSSVEKQVALLKALYGHGQIS↓HKR2428.26
P47867 Secretogranin-3K****R↓ELSAERPLNEQIAEAEADKI↓KK2225.12
P47867 Secretogranin-3K****R↓ELSAERPLNEQIAEAEAD↓KIKK1983.94
P56388 Cocaine- and amphetamine-regulated transcript proteinRA↓pyro-Glu(Q)EDAELQPR↓AL1067.49
Q03517 Secretogranin-2KR↓IPVGSLKNEDTPNRQYLDEDM↓LL2433.15
Q03517 Secretogranin-2KR↓IPVGSLKNEDTPN↓RQ1382.70
Q03517 Secretogranin-2KR↓SGQLGLPDEEN↓RR1157.52
Q03517 Secretogranin-2KR↓TNEIVEEQYTPQSL↓AT1649.78
Q03517 Secretogranin-2SVF↓pyro-Glu(Q)ELGKLTGPSNQ↓KRa1253.63
Q62361 Thyrotropin-releasing hormoneKR↓EEKEEDVEAEERGDLGEVGAWRPH↓KR2765.25
Q9QXV0 Pro-SAASRR↓SVDQDLGPEVPPENVL-amide↓GA1705.85
Q9QXV0 Pro-SAASRA↓WGSPRASDPPLAPDDDPDAPAAQLARAL↓LR2869.36
  • a Novel modification; sequence identified previously by Che et al. (2).