Table I

Proteins showing statistically significant expression changes upon T cell stimulation

Peptide identificationazPeptide quantification
Protein nameXcorrΔCnFDRPeptidelog2RRatiop valueFDR
%%
Actin 2.73 0.30 0.6 DSYVGDEAQSK +2 −3.67 1.0:12.7 0·E+00 0.0
Actin 4.07 0.38 0.0 VAPEEHPTLLTEAPLNPK +2 −1.87 1.0:3.6 3·E−08 0.0
Actin 2.86 0.33 0.3 EITALAPSTMK +2 −0.75 1.0:1.7 0.008 12
Histone H4 3.39 0.38 0.0 DAVTYTEHAK +2 −2.14 1.0:4.4 4·E−10 0.0
Histone H4 3.29 0.37 0.0 TVTAM*DVVYALK +2 −0.86 1.0:1.8 0.003 5
Histone H4 3.75 0.43 0.0 TVTAMDVVYALK +2 −0.52 1.0:1.4 0.05 58
Histone H43.790.400.0ISGLIYEETR+2−0.441.0:1.40.0970
Pleckstrin4.080.370.0SEEENLFEIITADEVHYFLQAATPK+3−0.881.0:1.80.0047
Cathepsin G3.710.160.3TIQNDIMLLQLSR+2−0.811.0:1.80.00812
Tubulin β-2 chain3.270.320.1AILVDLEPGTMDSVR+2−0.811.0:1.80.00711
l-Lactate dehydrogenase B chain4.650.420.0SLADELALVDVLEDK+2−0.811.0:1.70.00610
Calnexin3.910.350.0IPNPDFFEDLEPFR+2−0.761.0:1.70.00912
Vinculin3.690.540.0AIPDLTAPVAAVQAAVSNLVR+20.891.9:1.00.0117
Calmodulin3.280.410.0DGNGYISAAELR+20.901.9:1.00.00812
Calgranulin B, migration-inhibitory factor-related protein 143.610.450.0QLSFEEFIMLMAR+20.921.9:1.00.0047
Thrombospondin-1 3.39 0.34 0.1 GGVNDNFQGVLQNVR +2 0.76 1.7:1.0 0.03 32
Thrombospondin-1 4.94 0.54 0.0 IPESGGDNSVFDIFELTGAAR +2 1.06 2.1:1.0 0.003 6
Thrombospondin-1 3.60 0.43 0.0 FTGSQPFGQGVEHATANK +2 1.22 2.3:1.0 8·E−05 0.2
Thrombospondin-1 3.28 0.47 0.0 FVFGTTPEDILR +2 1.38 2.6:1.0 7·E−05 0.2
Actin 5.61 0.42 N/A DFEQEMATAASSSSLEK +2 1.97 3.9:1.0 6·E−10 0.0
  • a Parameters related to peptide identification are those obtained using SEQUEST as described previously by Lopez-Ferrer et al. (21). Repeated peptide assignments were removed for sake of clarity. Data related to proteins assumed to have significant expression changes, as explained in the text, are indicated in bold. Asterisks indicate oxidization of the preceding methionine residue. N/A, not applicable.