Table I

Precision of absolute quantification based on addition of peptide ion intensities

The intensities of peptides matching a particular protein were added up and expressed normalized to total protein in the gel section. The results of five replicate experiments analyzing the expression of one gel band containing a total cell lysate fraction are shown. Raw data are shown in Supplemental Fig. 2. n, number of peptides used for quantification per protein.

GI No.Protein NamenAmount of protein in gel section (ng/μg of total protein) in replicate experiment numberMeanS.D.CV%
12345
31543113Lymphocyte cytosolic protein 1297.610.113.113.914.411.82.924.5
98463665-kDa macrophage protein266.79.011.812.312.710.52.524.2
11066098Transketolase166.29.910.912.312.110.32.524.2
52783203Carboxykinase71.31.92.42.42.52.10.523.5
226021Growth-regulated nuclear 6861.21.41.81.91.81.60.319.3
56385SWAP-70171.11.01.03.910.13.43.9114.0
13242328Lymphocyte cytosolic protein 151.11.31.61.71.71.50.318.1
29368965-kDa macrophage protein61.11.61.72.02.11.70.425.0
16359229Transketolase80.91.21.41.51.51.30.319.8
16741161Carboxykinase60.60.80.81.01.10.80.223.4
19882225Growth-regulated nuclear 6840.50.70.91.01.00.80.224.4
2137619Lymphocyte cytosolic protein 120.30.40.50.50.60.50.124.0
232341065-kDa macrophage protein30.20.30.20.30.30.30.012.0