Table II

Sample recoveries for tandem IgY12 and SuperMix separations

Replicate^a	A	B	C	D	E	Recovery^b
						%
IgY12 flow-through (μg)^c	1249	1429	1281	1443	1457	10.0 ± 0.72
SuperMix bound (μg)^d	485	421	424	438	414	3.19 ± 0.21
SuperMix flow-through (μg)^d	121	128	123	117	122	0.89 ± 0.03
SuperMix flow-through postdigestion (μg)^d	68	54	69	78	50	0.47 ± 0.08

↵ ^a For each replicate experiment, 225 μl of plasma was used with the total protein quantity of ∼13.7 mg (plasma concentration, ∼61 mg/ml).
↵ ^b All recoveries are expressed as the percentage of recovered proteins or peptides compared with the 13.7 mg of starting materials along with the standard deviations.
↵ ^c The amount of proteins or peptides recovered for each replicate.
↵ ^d Only nine-tenths of the IgY12 flow-through was used for SuperMix separations.