Table III

Identified phosphorylation sites involved in the Wnt signaling pathway

Accession numberaGene nameLC-MS/MS run detectedbPrecursor massCharge statePeptidecProbabilitydFinal scoredXcorrdΔCNd
(Da)
FBgn0001139 gro 1 (DDA 2+)1,182.515062R↓NSVS*PADREK↓Y1.83 × 10−30.853.260.31
FBgn0001139 gro 1 (DDA 2+)1,356.675382R↓TRS*PLDIENDSK↓R1.52 × 10−60.823.280.43
FBgn0002783 mor 1 (DDA 2+)1,520.710462K↓ANAALQSTAS*PAPGGK↓S1.03 × 10−30.703.340.05
FBgn0015805 Rpd3 1 (DDA 2+)2,141.758412R↓IVPENEYS*DS*EDEGEGGR↓R2.53 × 10−50.956.440.09
FBgn0015805 Rpd3 1 (DDA 2+)2,297.859522R↓IVPENEYS*DS*EDEGEGGRR↓D2.41 × 10−40.564.650.14
FBgn0022131 aPKC 2 (DDA >2+)2,845.322673K↓EGIRPGDTTS*TFCGTPNYIAPEILR↓G1.20 × 10−30.534.040.30
FBgn0003371 sgg 2 (DDA >2+)1,852.841203K↓QLLHGEPNVSY*ICSR↓Y2.23 × 10−30.844.090.08
FBgn0003371 sgg 3 (INL-1)1,020.403382R↓TSS*FAEGNK↓Q8.72 × 10−30.551.920.29
FBgn0032157 CG5899 3 (INL-1)3,177.567493 K↓LLTTALGLDKDQEEQLNNSLNNSIAS*PAK 2.19 × 10−50.936.920.17
FBgn0032157 CG5899 4 (INL-2)1,981.629372K↓DQVYDS*DDS*DSEMSTK↓M2.29 × 10−40.955.600.09
FBgn0015589 Apc 4 (INL-2)2,738.398523 R↓RNS*VAGSGQNVDSPPVVIPASLQPLR↓S 2.30 × 10−40.533.820.09
FBgn0027492 wdb 5 (INL-NL)2,007.989932R↓YRNS*QDVELQQLPPLK↓A4.46 × 10−30.904.450.15
  • a Accession number as indicated in Flybase 4.3.

  • b Runs 1 (DDA 2+) and 2 (DDA >2+), only doubly or higher charged peptide ions, respectively, were allowed to trigger CID by DDA. Runs 3 (INL-1) and 4 (INL-2), directed LC-MS/MS of unsequenced features extracted from runs 1 and 2. Run 5 (INL-NL), directed LC-MS/MS of unassigned neutral loss-showing precursors.

  • c Phosphorylation sites are italic and followed by an asterisk.

  • d Search result values obtained from Bioworks 3.2.