Table I

X. laevis oocyte proteins identified by mass spectrometry using the GalNAz/biotin double labeling

The accession number, the bank used for the identification, the protein name, the apparent molecular mass found in SDS-PAGE, the theoretical molecular mass, the percentage of coverage, the Mascot score, and the number of peptides for each protein are reported. For each peptide, the charge state, the observed precursor m/z (Obs. M), the experimental (Exp. M) and theoretical (Cal. M) precursor neutral masses, the delta mass (ΔM), the number of missed cleavages, the peptide score value delivered by Mascot, and the peptide sequence are indicated. References in the last column of the table indicate the study relating the first description of the protein as bearing O-GlcNAc moieties.

Accession numberBank nameProtein nameApparent molecular massTheoritical molecular massCoverageMascot scoreNumber of peptidesChargeObs. MExp. M (molecular weight)Cal. MΔMMissed cleavagesScore, peptideSequencePrevious O-GlcNAc description
Da Da %
Cell shape and architecture
    TBB4_XENLASwiss-ProtTubulin β-4 chain54,95050,2401225952572.71143.391142.620.7688055K↓LAVNMVPFPR↓L
2665.11328.181327.640.5449053R↓INVYYNEATGGK↓Y
2801.811601.611600.810.7984068R↓AVLVDLEPGTMDSVR↓S
2846.711691.411690.860.5564076R↓ALTVPELTQQMFDAK↓N
2923.0118441842.921.0828192R↓INVYYNEATGGKYVPR↓A
    ACTB_XENLASwiss-Protβ-Actin42,24042,0821715452519.191036.381035.640.7399121K↓IKIIAPPER↓K32
2567.191132.371131.510.8549051R↓GYSFTTTAER↓E
2759.521517.041515.691.3477052K↓QEYDESGPSIVHR↓K
2896.251790.51789.880.6171060K↓SYELPDGQVITIGNER↓F
2977.831953.641953.060.5911054R↓VAPEEHPVLLTEAPLNPK↓A
Metabolism
    O129758XENLAMSDBAldolase39,66039,381613532533.231064.451063.550.9033056R↓QLLFTADEK↓V
2667.321332.631331.690.9403066K↓GILAADESTGSIAK↓R
2745.541489.081487.791.2897172K↓GILAADESTGSIAKR↓L
    G3P_XENLASwiss-ProtGlyceraldehyde-3-phosphate dehydrogenase44,68036,0172826551869.65868.64868.50.1473022K↓VIPELNGK↓I26, 27
11355.751354.741354.720.0271035R↓GAGQNIIPASTGAAK↓A
2779.421556.831555.81.0343088R↓VPTPNVSWDLTCR↓L
2844.751687.491686.930.5621065K↓LIINDQVITVFQER↓D
3870.042607.122605.321.8032059K↓VINDNFGIVEGLMTTVHAFTATQK↓T
    ENOA_XENLASwiss-Protα-Enolase51,40047,93024812572.491142.961142.60.3615048R↓IGAEVYHNLK↓N26, 27
    KPYK_XENLASwiss-ProtPyruvate kinase62,33057,4891218461856.68855.68855.510.1648026R↓APIISVTR↓N26
1964.72963.71963.550.1608019R↓GIFPVLYR↓E
2546.281090.551089.590.9632116R↓QLFEELRR↓V
2614.771227.531226.650.8831074R↓LDIDSEPIVAR↓N
2797.571593.131591.771.3624070R↓DPTEATAVGAVEASFK↓C
2807.921613.831612.711.1194060R↓EAVHEAWAEDVDSR↓V
    Q5XGY5_XENLAMSDBPyruvate dehydrogenase PdhE1β-2 protein31,57039,59549512902.011802.021800.891.1304095R↓VFLLGEEVAQYDGAYK↓I
    LDHA_XENLASwiss-Protl-Lactate dehydrogenase A chain31,42036,92588322602.571203.121202.620.5009083R↓IIGSGTNLDSAR↓F
2857.921713.831712.910.9166116R↓IVWTGGVRQQEGESR↓L
    LDHB_XENLASwiss-Protl-Lactate dehydrogenase B chain31,57036,3352231371943.7942.69942.590.1073028K↓FIIPQWK↓Y
2562.461122.911121.591.3202060K↓WDSAYEVIK↓L
2567.211132.421131.590.8307050K↓SAETLWGIQK↓D
2844.361686.711684.871.8367063K↓ELADELALVDILEDK↓L
3606.291815.861813.971.8938143K↓LKGEVMDLQHGSLFLK↓T
2919.861837.711836.920.7973185K↓TPTIVADKDYSVTANSR↓I
2964.561927.111926.051.0615165K↓ELADELALVDILEDKLK↓G
    Q8AVH2_XENLAMSDBCreatine kinase isozyme IV39,66042,4421110132617.571233.131231.61.5249026K↓DLFDPIIEDR↓H27
2754.781507.541506.690.8524082K↓GGDDLDPNYVLSSR↓V
2983.441964.881963.920.9591044R↓GTGGVDTAAVGGVFDVSNADR↓L
    Q918J7_XENLAMSDBTransketolase73,54068,3781119342641.261280.511280.67−0.1651021K↓ICPLVPTADAPK↓I
2861.751721.491720.880.6161086R↓SGKPTELLDMFGISAR↓C
21057.92113.792113.170.6133063R↓VIDPFTIKPLDAATILSSGR↓A
21234.162466.322465.241.0803160R↓TSRPDTAVIYSPEEKFEIGQAK↓V
    Q7SZ23_XENLAMSDBGlutathione S-transferase24,22025,5463413861962.67961.66961.530.1339023K↓NLQAFLTR↓F
2501.661001.311000.440.8666062K↓FSCFLGDR↓S
2675.61349.21347.681.5159041R↓ILDPTCLQNFK↓N
2794.611587.221585.781.4361054R↓LLLEYTGTQYEEK↓L
2938.571875.131873.981.1566034K↓LGLDFPNLPYLVDGDVK↓L
3748.342242.012240.071.937143K↓LYVTGDAPNYDKSQWLNEK↓E
    SAHHA_XENLASwiss-ProtAdenosylhomocysteinase B48,35048,1722429882511.0110201019.540.4616043K↓TGVPVYAWK↓G
2511.871021.731020.61.1255040R↓HIILLAEGR↓L
2602.211202.411201.60.8061044K↓VADISLADWGR↓K
2631.051260.091258.571.5167150K↓SKFDNLYGCR↓E
2691.461380.91379.741.1682170K↓KLDEAVAAAHLDK↓L
2825.23648.441647.810.6364069K↓GISEETTTGVHNLYK↓M
2915.781829.561828.950.61063K↓SSGTLQVPAINVNDSVTK↓S
3789.912366.72365.191.5135067K↓DGKPLNMILDDGGDLTNLVHTK↓Y
Translation
    EF1A2_XENLASwiss-ProtElongation factor 1-α, oocyte form 3-151,40050,43377122513.591025.171024.60.5723071K↓IGGIGTVPVGR↓V31
3840.092517.262516.350.909907R↓VETGVLKPGMIVTFAPSNWTTEVK↓S
    RS31_XENLASwiss-Prot40 S ribosomal protein S3-A31,57027,1563922172516.091030.171028.621.554059R↓TEIIILATR↓T
2547.231092.441091.560.8851051K↓AELNEFLTR↓E
2572.881143.741142.571.1688032K↓IMLPWDPSGK↓I
2712.71423.381422.660.7271059R↓ELAEDGYSGVEVR↓V
2787.521573.031571.751.284065R↓FGFPEGSVELYAEK↓V
2958.661915.311913.961.3483032K↓AAKPDQPQPPAMPQPVATA
3824.052469.132467.181.9528055K↓FVDGLMIHSGDPVNYYVDTAVR↓H
    RS32_XENLASwiss-Prot40 S ribosomal protein S3-B31,57027,1324018562547.231092.441091.560.8851051K↓AELNEFLTR↓E
2572.881143.741142.571.1688032K↓IMLPWDPSGK↓I
2712.71423.381422.660.7271059R↓ELAEDGYSGVEVR↓V
2787.521573.031571.751.284065R↓FGFPEGSVELYAEK↓V
2965.81929.61927.981.6193030K↓GAKPDQPQPPVMPQPVATA
3824.052469.132467.181.9528055K↓FVDGLMIHSGDPVNYYVDTAVR↓H
    RL4A_XENLASwiss-Prot60 S ribosomal protein L4-A44,68045,1921914952634.31266.581265.640.9461161R↓KLDDLYGTWR↓K
2635.191268.361267.750.6165043R↓NIPGITLLNVSK↓L
3520.541558.611556.731.8783029K↓LAGHQTSAESWGTGR↓A
3621.961862.861861.031.8318033R↓APIRPDIVNFVHTNLR↓K
2969.331936.641936.010.6315066R↓YAVCSALAASALPALIMSK↓G
    RL5A_XENLASwiss-Prot60 S ribosomal protein L5-A33,91034,18175712670.411338.811337.691.1225057K↓GAVDGGLSIPHSTK↓R
    RL18A_XENLASwiss-Prot60 S ribosomal protein L18-A23,20021,73555612571.631141.261140.620.635056K↓GQNTVLLSGPR↓K
Other function
    PCNA_XENLASwiss-ProtProliferating cell nuclear antigen31,57028,87878121933.09932.08931.480.6052031R↓YLNFFTK↓A
2634.181266.351265.620.7294072K↓FSASGELGTGNVK↓L
    TERA_XENLASwiss-ProtTransitional endoplasmic reticulum ATPase88,83089,76011183611021.681020.681020.510.1676042K↓DVDVDFLAK↓M
2526.811051.61050.511.091065K↓MDELQLFR↓G
2587.241172.461171.660.807032R↓GILLYGPPGTGK↓T
2771.261540.521539.790.7254039R↓LGDVISIQPCPDVK↓Y
2790.321578.631577.780.8443049K↓AIANECQANFISK↓G
2913.161824.321822.911.4128057R↓ELQELVQYPVEHPDK↓F
    Q7ZWU1_XENLAMSDBStress-induced phosphoprotein (Hop)73,54062,50627312709.191416.371415.760.6111073R↓VAYINPDLALEAK↓N
    JC4316MSDBPhosphoprotein phosphatase 2A-β catalytic chain31,42036,12634012671.341340.671339.651.0152040K↓YSFLQFDPAPR↓R
    RAN_XENLAMSDBGTP-binding nuclear protein ran26,100–24,22024,55445522608.071214.141213.590.5442035K↓NLQYYDISAK↓S26
2648.41294.791293.591.1968055K↓FNVWDTAGQEK↓F