Table I

Reproducibility of directed LC-MS/MS

No. of observationsFeature intensityaNo. of features targetedbNo. of features sequencedNo. of features identified
1All1,000984945
2All1,000972906
3All1,000953863
4All1,000923803
5All1,000865695
5Very high200200192
5High200186164
5Medium200170129
5Low200161117
5Very low20014893
  • a Feature intensity bins based on feature area determined by SuperHirn (very high, 2.6 × 109–3 × 107; high, 3.0-1.4 × 107; medium, 1.4 × 107–8.1 × 106; low, 8.1-4.9 × 106; and very low, 4.9 × 106–5.3 × 105).

  • b The same 1,000 features were targeted in each run, including 200 features of each intensity bin.