Table I

Proteins differentially recognized by l-PHA on 2-DE gels and identified by ESI-MS spectrometry and a Mascot blast search against NCBInr

Accession gi no.IdentitiesPeptides matchedSequence coverage (%)Total scoreaMr/pIbLevelsc (±S.D.)
177836 α-1-antitrypsin precursor726.343846.7/5.50.26 (0.03)
15079348 Angiotensinogen preproprotein310.118953.1/5.81.19 (0.07)
4261632 β-N-acetylhexosaminidase A1022.357560.7/5.01.77 (0.17)
54697170 Cathepsin D preproprotein511.732044.5/6.13.87 (0.24)
3650498 Cathepsin X precursor25.910733.9/7.11.19 (0.11)
68533097 DDR1 variant protein34.414199.0/6.30.66 (0.06)
16877430 Dipeptidyl peptidase 7 preproprotein311.314654.3/5.92.33 (0.17)
38327632 Discoidin receptor tyrosine kinase26.19996.9/6.11.40 (0.12)
4758116 Dystroglycan 1 precursor23.310697.6/8.71.42 (0.15)
5031863 Galectin 3 binding protein36.814765.3/5.10.53 (0.06)
4504151 Granulin isoform 1 precursor35.714963.5/6.41.21 (0.09)
5729877 Heat shock 70 kDa protein 8 isoform 1714.637970.9/5.42.47 (0.15)
15010550 Heat shock protein gp96 precursor912.048490.2/4.70.34 (0.04)
11602963 Heparan sulfate proteoglycan perlecan31.1171466.6/6.03.26 (0.33)
16924217 Hexosaminidase B preproprotein510.426663.1/6.30.87 (0.06)
5080756 Human Fc binding protein31.6179572.1/5.10.19 (0.02)
106586 Ig kappa chain V-III325.125223.1/5.81.48 (0.11)
9845498 Laminin γ1 precursor118.7746177.6/5.01.47 (0.06)
56682964 Legumain preproprotein312.715249.4/6.11.54 (0.11)
51095116 Met proto-oncogene75.3471155.4/7.01.76 (0.04)
4503899 N-acetylgalactosamine-6-sulfatase514.929558.0/6.30.63 (0.06)
4504061 N-acetylglucosamine-6-sulfatase817.945762.0/8.60.18 (0.02)
57209715 Prosaposin510.931858.1/5.12.01 (0.08)
4505989 Protective protein for β-galactosidase25.417854.5/6.20.35 (0.04)
2136061 Protein-tyrosine kinase-related receptor PTK779.2432118.3/6.70.47 (0.02)
57160745 Protein tyrosine phosphatase kappa77.8374162.0/5.62.04 (0.07)
5231228 Ribonuclease T2 precursor525.326229.5/6.70.86 (0.07)
57210053 Tissue inhibitor of metalloproteinase-1426.121523.2/8.51.97 (0.06)
120749 Tumor-associated calcium signal transducer 1211.818434.9/7.41.08 (0.09)
38026 Zn-α-2-glycoprotein419.523434.7/5.70.41 (0.05)
  • a Total score is a sum of the score values obtained from each of an individual peptide. Score is −10 x Log (P), where P is the probability that the observed match is a random event; it is based on NCBInr database using the MASCOT searching program as MS/MS data.

  • b Molecular weight (Mr) and isoelectric point (pI) are theoretical values where glycan residues were not considered for the calculations. The theoretical values are prone to be changed by an attachment of glycans to peptides and thus to be different from the experimental values estimated on two-dimensional electrophoresis gels.

  • c Numbers refer to the relative levels of each protein from WiDr:GnT-V compared to those from WiDr:mock (n = 5).