Table II

Inhibition constants for active gelatinase/TIMP-1 interactions

TIMP-1Gelatinaseskon (M−1·s−1×105)koff (s−1×103)Ki (nm)
TIMP-1: mock62 kDa MMP-24.99 ± 0.722.71 ± 0.475.43 ± 1.39
82 kDa MMP-97.07 ± 0.642.26 ± 0.433.20 ± 1.23
TIMP-1: GnT-V62 kDa MMP-20.98 ± 0.063.83 ± 0.2439.1 ± 8.38
82 kDa MMP-90.75 ± 0.072.74 ± 0.3936.5 ± 6.54