Table I

Cerebral cortex protein alterations in response to treatment with NMDAR antagonist dizocilpine in infancy

For each altered isoprotein, an abbreviation, the 2-DE spot number, the Swiss-Prot/TrEMBL accession number and/or NCBI reference number, the expression group (S, stable; E, early; L, late; T, transient), the number of peptides identified through ESI-MS, the sequence coverage (Seq. cov.), an ESI-MS identification score, and the percentage of difference of an individual spot between treated mice and controls are presented. For mobility variants (mV), the numbers of mouse pairs showing this alteration are given in parentheses. Triangles pointed upward (▴) and downward (▾) indicate an increase or decrease, respectively, of a spot intensity in treated mice as compared with their control littermates.

Accession no.Swiss-Prot no.Spot no.ProteinEGPeptide no.Seq. cov.ScoreQuantitative changes
P7 acuteP14 post-1 weekP35 post-4 weeks
A. Proteins involved in oxidative stress and apoptosis
    PARK7_MOUSEQ99LX05640494422DJ-1 protein (DJ1)S1761128▾ 29a
    PRDX1_MOUSEP3570054792340Peroxiredoxin 1 (PRDX1)S1028519▴ 42b
    CAH2_MOUSEP0092014634538337Carbonic anhydrase 2 (CA2)L1770166▴ 38c
    QOR_MOUSEP47199117280150Zeta crystallin, quinone oxidoreductase (CRYZ)L1245131▾ 18b
    LGUL_MOUSEQ9CPU02136264017/39/57Glyoxalase 1 (GLO1)S434247mV (9/11)mV (6/9)mV (10/11)
B. Proteins involved in growth and energy metabolism
    PSA5_MOUSEQ9Z2U11222995314Proteasome subunit, α type 5 (PSAM5)S312155▾ 10c
    EIF3G_MOUSEQ9Z1D1235030736Eukaryotic translation initiation factor 3, subunit 4 (EIF3g)S49210▴ 21a
    GUAD_MOUSEQ9R1119910725Guanine deaminase (GDA)E933511▾ 12b
    GBB2_MOUSEP62880513173057G protein β2 subunit (GNB2)L72773▾ 79b
    GBB2_MOUSEP62880513173058G protein β2 subunit (GNB2)L1336106▾ 48c
    GFAP_MOUSEP039951463454234Glial fibrillary acidic protein, astrocyte (GFAP)S2259265▾ 12c
    GMFB_MOUSEQ9CQI34657664026Glia maturation factor, beta (GMFB)E74178▴ 14b
    NP_067248.1109465745Creatine kinase, brain (CKB)L1646117▾ 63a
    MDHM_MOUSEP0824914634545711Malate dehydrogenase, mitochondrial precursor (MDH2)L62364▾ 21b
    NP_033989.22817355031Cell division cycle 10 homolog (CDC10)L3764317▾ 18b
    Q3TI62_MOUSEQ3TI6212378665246Chaperonin subunit 6a (CCT6)S1634134▴ 14c
    TERA_MOUSEQ0185314629107843Transitional endoplasmic reticulum ATPase, VCPS4444369▾ 17c
    Q6GTD3_MOUSEQ6GTD38188630651NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9 (NDUFA9)S2057217▴ 29c
C. Proteins involved in synapse function/vesicular transport
    CPLX2_MOUSEP840865131699624Complexin 2 (CPLX2)S94876▾ 13c
D. Proteins involved in neuronal migration, axon growth, and guidance
    DPYL1_MOUSEP97427312203025Collapsin response mediator protein 1 (CRMP1)E1764▴ 38b
    DPYL1_MOUSEP9742731220301Collapsin response mediator protein 1 (CRMP1)E2753169▴ 78a
    COF1_MOUSEP1876011684927Cofilin 1, non-muscle (COF1)S327258▴ 37c
    MAP1B_MOUSEP1487312674513Microtubule-associated protein 1B (MAP1B)S83168▴ 18b
    MAP1B_MOUSEP1487312674553Microtubule-associated protein 1B (MAP1B)L93087▾ 8c
    NP_032801.24025462418Platelet-activating factor acetylhydrolase, isoform 1b, α2 subunit (PAFAH1B2)S93689▴ 13b
    RSSA_MOUSEP142061463455072Laminin receptor 1 (LAMR1)S625401▴ 18c
    PHB_MOUSEP677785403883734Prohibitin (PHB)S2271281▴ 10b
    PHB_MOUSEP677785403883719/35/55Prohibitin (PHB)S2176241mV (10/11)mV (8/9)mV (9/11)
    RANG_MOUSEP340224639782816Ran-binding protein 1 (RANBP1)S317152mV (10/11)
    GDIR1_MOUSEQ99PT12175913015/38/56Rho GDP dissociation inhibitor 1 (RHOGDI1)S739453mV (8/11)mV (6/9)mV (9/11)
    GDIA_MOUSEP503964711785544Rab GDP dissociation inhibitor 1 (RABGDI1)S1130594▴ 37c
    KHDR1_MOUSEQ607496251110829KH domain-containing, RNA-binding, signal transduction-associated protein 1 (KHDRBS1)S1517104▴ 13b
    COF1_MOUSEP1876011684942Cofilin-1 (CFL1)E460242▴ 36c
    FSCN1_MOUSEQ6155314634542147Fascin (FSCN1)S1857192▴ 25c
    FSCN1_MOUSEQ6155314634542148Fascin (FSCN1)S2855240▴ 25c
    PROF2_MOUSEQ9JJV21312447060Profilin 2 (PFN2)S64473▾ 11c
    ARPC2_MOUSEQ9CVB611082570652Actin-related protein 2/3 complex subunit 2 (ARPC2)T1542171▴ 14a
    NP_038692.1730548532/49SH3-domain GRB2-like 1 (SH3GL1)L1133596▴ 31a▴ 27b
    MYL6_MOUSEQ606054760644259Smooth muscle and nonmuscle myosin light chain alkali 6 (MYL6)L86672▾ 46b
    NP_075891.12172837623/41/58Myosin regulatory light chain-like (MRLCL)S638416mV (10/11)mV (7/9)mV (10/11)
E. Other proteins
    PDXK_MOUSEQ8K183612298419Pyridoxal kinase (PDXK)L1137103▾ 13b
    APOA1_MOUSEQ0062323155720Apolipoprotein A-1 precursor (APOA1)S1342100▴ 16b
    APOA1_MOUSEQ0062323155721Apolipoprotein A-1 precursor (APOA1)S1649145▾ 11c
    NP_082695.12131215328RIKEN cDNA 2900070E19L219132▾ 13b
    ROA3_MOUSEQ8BG053031620110Heterogeneous nuclear ribonucleo protein A3 (HNRNPA3)S2658178▴ 29a
    ROA3_MOUSEQ8BG053031620133Heterogeneous nuclear ribonucleo protein A3 (HNRNPA3)S2853202▴ 26b
    NMRL1_MOUSEQ8K2T18190102912/36NmrA-like family domain containing 1 (NMRA1)S1924276mV (9/11)mV (7/9)
    Q8CIB4_MOUSEQ8CIB48187819230Aldehyde dehydrogenase family 6, subfamily A1 (ALDH6A1)L2553260▴ 26c
    PUR9_MOUSEQ9CWJ9146345497455-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (ATIC)S2856265▴ 20a
    Q8R5L1_MOUSEQ8R5L18187917454Complement component 1, q subcomponent-binding protein C1QBPS63165▾ 14b
  • a p < 0.001.

  • b p < 0.01.

  • c p < 0.05.