Table II

Cerebral cortex protein alterations in response to treatment with GABAAR agonist phenobarbital in infancy

For each altered isoprotein, an abbreviation, the 2-DE spot number, the Swiss-Prot/TrEMBL accession number and/or NCBI reference number, the expression group (S, stable; E, early; L, late; T, transient), the number of peptides identified through ESI-MS, the sequence coverage (Seq. cov.), an ESI-MS identification score, and the percentage of difference of an individual spot between treated mice and controls are presented. For mobility variants (mV), the numbers of mouse pairs showing this alteration are given in parentheses. Triangles pointed upward (▴) and downward (▾) indicate an increase or decrease, respectively, of a spot intensity in treated mice as compared with their control littermates.

Accession no.Swiss-Prot no.Spot no.ProteinEGPeptide no.Seq. cov.ScoreQuantitative changes
P7 acuteP14 post-1 weekP35 post-4 weeks
A. Proteins involved in oxidative stress and apoptosis
    PRDX1_MOUSEP3570054792322Peroxiredoxin 1S731371▴ 7a
    1433E_MOUSEP62259603911921614-3-3 protein, ε polypeptide (14-3-3ε)S23691102▴ 71b
    1433G_MOUSEP61982484287221814-3-3 protein, γ polypeptide (14-3-3γ)S1553830▴ 152a
    LGUL_MOUSEQ9CPU02136264040Glyoxalase 1 (GLO1)S434247▴ 14a
B. Proteins involved in growth and energy metabolism
    NACA_MOUSEQ608177115199711Nascent polypeptide-associated complex subunit α (NACA)E131108▴ 26c
    NACA_MOUSEQ608177115199712Nascent polypeptide-associated complex subunit α (NACA)E425301▴ 24c
    NACA_MOUSEQ608177115199713Nascent polypeptide-associated complex subunit α (NACA)E319218▴ 17c
    NACA_MOUSEQ608177115199714Nascent polypeptide-associated complex subunit α (NACA)E319270▾ 14b
    PRS7_MOUSEP46471205324109Proteasome 26 S subunit ATPase 2 (PSMC2)S1233653▴ 56a
    PSD11_MOUSEQ8BG325278322910Proteasome 26 S subunit ATPase subunit 11 (PSMD11)L25103▴ 10b
    CAA51209.139738717Tropomyosin 5 (TPM5)L21531118▴ 38a
    NP_067248.1109465748Creatine kinase, brain (CKB)L1647867▴ 144a
    AAL02400.1165801282Dihydrolipoamide S-acetyltransferase precursor (DLAT)L513236▴ 36a
    NDUA4_MOUSEQ624252350309024NADH dehydrogenase (ubiquinone) 1α subcomplex, 4 (NDUFA4)S336165▾ 12a
    CAI24120.15620690225NADH dehydrogenase (ubiquinone) Fe-S protein 1 (NDUFS1)S2029946▾ 34b
    PHB_MOUSEP677785403883719/34Prohibitin (PHB)S1252781▴ 25b▾ 78c
    PHB_MOUSEP677785403883738Prohibitin (PHB)S1252781▾ 78c
    NP_032836.1667926130Pyruvate dehydrogenase E1 α1 (PDHA1)T1024447▾ 38a
    DHSA_MOUSEQ8K2B35278278526Succinate dehydrogenase flavoprotein subunit, mitochondrial (SDHA)L1421680▾ 14a
    EIF1_MOUSEP48024740446631Eukaryotic translation initiation factor 1 α1E59248▴ 53a
    AK1A1_MOUSEQ9JII62265362833Aldo-keto reductase family 1 member A4 (AKR1A4)1232583▴ 16b
    VDAC3_MOUSEQ60931609377036Voltage-dependent anion channel 3 (VDAC3)S837540▾ 14b
    NP_033989.2281735506Cell division cycle 10 homolog (CDC10)L1126578▾ 12b
    NP_033989.22817355044Cell division cycle 10 homolog (CDC10)L817432▴ 14a
    TERA_MOUSEQ0185314629107843Transitional endoplasmic reticulum ATPase, VCPS20271207▾ 11b
C. Proteins involved in synapse function/vesicular transport
    GFAP_MOUSEP039951463454237Glial fibrillary acidic protein, astrocyte (GFAP)S22481341▴ 13b
    GFAP_MOUSEP0399514634542327Glial fibrillary acidic protein, astrocyte (GFAP)S26521445▴ 25a
    XP_619816.16349240837Similar to synaptosomal-associated protein 25 isoform SNAP25A (SIMSNAP2)S1044523▴ 10b
    PEBP1_MOUSEP702962984083939Phosphatidylethanolamine-binding protein (PEBP)S544344▴ 12b
D. Proteins involved in neuronal migration, axon growth, and guidance
    DPYL1_MOUSEP9742731220305Collapsin response mediator protein (CRMP1)E1122565▴ 40a
    DPYL2_MOUSEO08553947303761Collapsin response mediator protein (CRMP2)E616287▴ 23c
    NP_033494.166812194Collapsin response mediator protein (CRMP3)S1124533▴ 81b
    NP_033494.166812193Collapsin response mediator protein (CRMP4)E921510▴ 132b
    ACTZ_MOUSEP611644711765229ARP1 actin-related protein 1 homolog A, centractin alpha (ACTR1)S1029603▾ 16b
    NP_075891.12172837641Myosin regulatory light chain-like (MRLCL)S638382mV (6/9)
    TCTP_MOUSEP630285170332838Translationally controlled tumor protein (TPT1)S421213▴ 9a
    TCTP_MOUSEP630285170332845Translationally controlled tumor protein (TPT1)S520231▴ 16b
    PROF2_MOUSEQ9JJV21312447046Profilin 2 (PFN2)S744391▾ 27a
E. Other proteins
    LZIC_MOUSEQ8K3C38191467421Leucine zipper and CTNNBIP1 domain-containing protein (LZIC)S745408▴ 18c
    NP_077183.11815279315Pyruvate dehydrogenase (lipoamide) β (PDHB)L718304▴ 60b
    O08855_MOUSEO088558187037820Apolipoprotein A-1 (APOA1)S929465▾ 30a
    BAE22051.17414089123Unnamed proteinS791462▴ 12a
    PRVA_MOUSEP32848333447842Parvalbumin (PRVA)L753362▾ 11c
    NP_038692.1730548528SH3-domain GRB2-like 1 (SH3GL1)S29125▴ 35c
    BAE31148.17421461335Unnamed proteinT1034454▾ 14b
    NP_083849.11825028432Isocitrate dehydrogenase 3 (NAD+) α (IDH3A)L410242▴ 20b
  • a p < 0.01.

  • b p < 0.05.

  • c p < 0.001.