Table II

Kinetic parameters and limits of detection for four arrayed antibodies

A series of dilutions of four serum proteins in PBS was applied to the high density array. Kinetic parameters for a two-compartment model were determined by curve fitting. kD, dissociation constant; LOD, limit of detection.

OrganismProteinMolecular mass, kDaAntibodyComponent 1Component 2LODConcentration
kfwd, M−1s−1krev, s−1kD, nmResponse, μRIUkfwd, M−1s−1krev, s−1kD, nmResponse, μRIUng/mlpmμg/mlμm
HumanTransferrin80HuECT3.2 × 1055.4 × 10−41.7253.71.4 × 1041.8 × 10−312574.0141752.0625.7
HumanAlbumin67Hu455.9 × 1051.0 × 10−41.7215.65.4 × 1034.3 × 10−479.441.95075024.3362
MouseTransferrin80Hu319.0 × 104<10−4<1.1181.85.4 × 1051.2 × 10−222.959.1587251.1814.8
MouseAlbumin69Hu452.8 × 105<10−4<0.3624.94.1 × 1042.5 × 10−360.733.65275523.6342