Table III

Serum protein cross-reactivity

The affinity was determined for all SPR array antibodies reacting detectably with mouse and human forms of albumin and transferrin. For the nearly 400 different antibodies arrayed, only 19 instances of off-target binding were observed. In all cases, the fastest on-rates were exhibited by the specifically targeted antigens. Affinity was calculated from the kinetic rates assuming a simple, one-compartment model. Hum, human; Mono, monoclonal antibody; Poly, polyclonal antibody; ASGPR, asialoglycoprotein receptor; ASL, argininosuccinate lyase.

Analyte nameAntibody targetCodeForFromTypekfwdkrevAffinityResponse
M−1 s−1 nm μRIU
Mouse albuminAlbuminHu45MouseRabbitPoly610,0007.92 × 10−41.3048.1
Mouse albuminAlbuminAACHumGoatMono17,5009.73 × 10−455.751.4
Mouse albuminTransferrinHu31Hum, mouse, ratChickenPoly6,4109.89 × 10−415444.3
Mouse albuminC4 complementH336671.18 × 10−217,700>600
Mouse albuminTransferrinHu43MouseGoatPoly2592.21 × 10−38,530526
Mouse transferrinTransferrinHu43MouseGoatPoly123,0002.19 × 10−317.964.0
Mouse transferrinTransferrinHu31Hum, mouse, ratChickenPoly98,0001.80 × 10−318.4165
Mouse transferrinApolipoprotein BHu29Hum, mouseRabbitPoly65,4001.00 × 10−315.321.9
Mouse transferrinTransferrinATCHumGoatMono64,6002.76 × 10−342.643.9
Mouse transferrinTransferrinHu81HumMouseMono35,7006.59 × 10−418.536.6
Human albuminAlbuminHu45MouseRabbitPoly386,0001.08 × 10−32.8025.3
Human albuminAlbuminAACHumGoatMono74,6003.02 × 10−44.05189
Human albuminASGPRHu30RatMouseMono46,5009.87 × 10−421.289.9
Human albuminHaptoglobinHu32Hum, mouse, ratChickenPoly38,700<10−42.59565
Human albuminTrypsinHu35Hum, mouse, ratChickenPoly11,500<10−48.68116
Human albuminTransferrinHu81HumMouseMono8,5702.67 × 10−431.2167
Human albuminTransferrinHu31Hum, mouse, ratChickenPoly5,8301.61 × 10−327557.2
Human albuminASLHu51HumMouseMono3,4601.01 × 10−329295.5
Human albuminTransferrinATDHumGoatMono3,2302.11 × 10−365391.9
Human transferrinTransferrinATCHumGoatMono278,0005.59 × 10−42.0162.2
Human transferrinTransferrinHu31Hum, mouse, ratChickenPoly110,0001.44 × 10−41.30357
Human transferrinTransferrinATDHumGoatMono94,3001.10 × 10−311.7114
Human transferrinAlbuminAACHumGoatMono61,7002.54 × 10−44.11209
Human transferrinAlbuminAADHumGoatMono53,6001.92 × 10−335.789.3
Human transferrinTrypsinHu35Hum, mouse, ratChickenPoly36,5005.71 × 10−415.6112
Human transferrinFerritinHu34HumMouseMono35,9002.01 × 10−356.029.2
Human transferrinHaptoglobinHu32Hum, mouse, ratChickenPoly31,9006.37 × 10−52.00353
Human transferrinCD16/32CD16HumMouseMono22,5002.15 × 10−395.540.6
Human transferrinC3 complementACCHumChickenMono21,6003.12 × 10−414.5227
Human transferrinC3 complementHu27Hum, mouse, ratSheepPoly20,0005.31 × 10−426.633.4