Table I

Comparison of the peptides detected by ESI-MS from uroplakins II and III after conventional in-gel digestion or on-membrane digestion after Western blot analysis

ND, not detected.

m/z sequenceScorea
IGbOM/NC-freec
Uroplakin II
1033AESIGLAMAR + Ox(M)d3113
1092EIPMSTFPR + Ox(M)3023
1145KAESIGLAMAR + Ox(M)3475
1148YYSYLVTK3931
1462LSAYQVTNLAPGTK9291
1651ELVSVVDSGSGFTVTR7996
Uroplakin III
1261ASEILNAYLIR6081
1491LTLYSAIDTWPGRND81
1496SLGTSEPSYTSVNR7832
1506TPLSSTFQQTQGGR89104
2025GDADGATSHDSQITQEAVPKND96
  • a Mascot score.

  • b Conventional in-gel digestion.

  • c On-membrane digestion carried out after Western blot analysis.

  • d Oxidation of methionine.