Table I

Representative phosphorylation sites that display large changes in stoichiometry between the cerebellum and hippocampus

637 phosphorylated sites were quantified in both biological replicates. The table lists those sites showing a high, reproducible level of differential phosphorylation in the hippocampus relative to the cerebellum. The top portion of the table lists the 10 phosphorylation sites showing the highest hippocampus:cerbellum ratio; the bottom portion shows the 9 sites with the lowest ratio. Lowercase "s, t, or y" designates the site of modification. "Acetyl-" refers to protein amino terminal acetylation, and lowercase "m" refers to oxidation of methionine. Parentheses designate ambiguity regarding the exact site of modification. The relative stoichiometries in each brain region are listed along with the S.D. from both replicates in parentheses. The final column lists the ratio of relative phosphorylation in the hippocampus versus cerebellum. Hip, hippocampus; Cer, cerebellum.

Protein namePeptideRelative stoichiometry
CortexMidbrainCerebellumHippocampusHip/Cer
Densin 180(sYstEsYGAsQtRPVsARPt)mAALLEK0.75 (0.14)0.60 (0.12)0.25 (0.05)1.55 (0.03)6.22
Calcium channel α1EFGEAVVVGRPGsGDGDSDQSR1.31 (0.07)0.87 (0.10)0.48 (0.17)1.46 (0.21)3.06
Densin 180SQsIDEIDVGTYK0.83 (0.07)0.83 (0.01)0.49 (0.17)1.31 (0.07)2.67
Calcium channel γ3DLsPISK1.48 (0.61)0.99 (0.08)0.38 (0.20)1.02 (0.33)2.66
Liprin, α3VSSSGLDsLGR0.97 (0.14)0.89 (0.03)0.54 (0.08)1.44 (0.03)2.65
Amino acid transporter 4Acetyl-(ss)HGNSLFLR1.16 (0.23)0.90 (0.39)0.62 (0.22)1.61 (0.39)2.62
D15Wsu169eFL(sLEYs)PVGK0.48 (0.33)0.64 (0.09)0.66 (0.21)1.61 (0.19)2.46
CNK2QEVTG(ssAVs)PIRK1.01 (0.29)0.61 (0.04)0.60 (0.18)1.41 (0.05)2.36
Liprin, α3sLPGSALELR1.19 (0.16)0.93 (0.20)0.53 (0.07)1.24 (0.01)2.31
Munc13-1ESYSDSmHsYEEFSEPR0.96 (0.44)0.88 (0.14)0.59 (0.05)1.34 (0.21)2.29
GluR δ-2APNGGFFRsPIK0.80 (0.20)0.86 (0.12)1.16 (0.13)0.58 (0.28)0.50
Connexin 43VAAGHELQPLAIVDQRP(ss)R0.84 (0.33)1.01 (0.03)1.70 (0.41)0.59 (0.26)0.35
Ankyrin 3RQsFASLALR0.84 (0.08)1.02 (0.29)1.44 (0.12)0.65 (0.29)0.46
CaMKII, αmLtINPSK1.22 (0.02)1.19 (0.12)1.54 (0.24)0.69 (0.05)0.45
CaMKII, αITAAEALKHPWIsHR1.14 (0.06)1.28 (0.22)1.57 (0.25)0.69 (0.14)0.44
CaMKII, αLKGAILTtmLATR0.99 (0.03)1.14 (0.11)2.44 (0.58)0.64 (0.16)0.26
CaMKII, αAGAYDFPsPEWDTVTPEAK1.06 (0.07)1.58 (0.12)1.57 (0.30)0.63 (0.02)0.40
Solute carrier family 12EIQSI(tDEsRGs)IR0.40 (0.02)0.82 (0.13)1.70 (0.00)0.50 (0.16)0.29
Diacylglycerol lipase, αLLsPVAAASAAR0.20 (0.11)0.34 (0.12)1.61 (0.14)0.15 (0.04)0.09