Table III

Comparison of quantification results between global proteomics experiments, quantitative RT-PCR, and targeted MRM experiments for noggin treatment versus control in hESCs

Ranges for qRT-PCR are shown in parentheses. N/A, not applicable.

ProteinRatio noggin vs. control
GlobaliTRAQ MRMqRT-PCR
Cytokeratin-80.92, p = 0.331.02, p = 0.25↓5.28, S.D. = 0.8 (↓3.12–↓8.92)
Tubulin β-III1.06, p = 0.701.08, p = 0.24N/A
CRMP21.17, p = 0.091.05, p = 0.10↓1.10, S.D. = 0.8 (↓1.66–↑1.38)
CRMP41.21, p < 0.001.25, p = 0.03↑1.55, S.D. = 0.5 (↑1.08–↑2.22)
NASP1.11, p = 0.201.00, p = 0.47↓3.14, S.D. = 0.6 (↓2.11–↓4.67)
MMIF1.16, p = 0.201.05, p = 0.10N/A