Comparison of quantification results between global proteomics experiments, quantitative RT-PCR, and targeted MRM experiments for noggin treatment versus control in hESCs
Ranges for qRT-PCR are shown in parentheses. N/A, not applicable.
| Protein | Ratio noggin vs. control | ||
|---|---|---|---|
| Global | iTRAQ MRM | qRT-PCR | |
| Cytokeratin-8 | 0.92, p = 0.33 | 1.02, p = 0.25 | ↓5.28, S.D. = 0.8 (↓3.12–↓8.92) |
| Tubulin β-III | 1.06, p = 0.70 | 1.08, p = 0.24 | N/A |
| CRMP2 | 1.17, p = 0.09 | 1.05, p = 0.10 | ↓1.10, S.D. = 0.8 (↓1.66–↑1.38) |
| CRMP4 | 1.21, p < 0.00 | 1.25, p = 0.03 | ↑1.55, S.D. = 0.5 (↑1.08–↑2.22) |
| NASP | 1.11, p = 0.20 | 1.00, p = 0.47 | ↓3.14, S.D. = 0.6 (↓2.11–↓4.67) |
| MMIF | 1.16, p = 0.20 | 1.05, p = 0.10 | N/A |