Table IV

Comparison of quantification results between global proteomics experiments, quantitative RT-PCR, and targeted MRM experiments for BMP4 treatment versus control in hESCs

Ranges for qRT-PCR are shown in parentheses. N/A, not applicable.

ProteinRatio BMP4 vs. control
GlobaliTRAQ MRMqRT-PCR
Cytokeratin-83.44, p < 0.006.93, p < 0.00↑64.74, S.D. = 0.4 (↑48.86–↑85.80)
Tubulin β-III0.80, p < 0.000.58, p < 0.00N/A
CRMP20.72, p < 0.000.55, p = 0.03↓4.19, S.D. = 0.4 (↓3.25–↓5.40)
CRMP40.71, p < 0.000.32, p = 0.02↓4.39, S.D. = 0.3 (↓3.51–↓5.48)
NASP0.85, p = 0.020.57, p = 0.02↓5.34, S.D. = 0.2 (↓4.58–↓6.22)
MMIF0.95, p = 0.600.71, p = 0.10N/A