Table II

Selected proteins involved in outer segment structure, metabolism, and vesicular trafficking

The number of unique peptides used for the identification is given. The protein to rhodopsin ratio was estimated by APEX analysis. NF, not found in the APEX list of 150 most abundant proteins in the primary data set.

ProteinUnique peptidesProtein to rhodopsin ratio (APEX)
Structure
    Peripherin/rds140.141
    rom-1110.248
    Prominin-118NF
    Retinitis pigmentosa 1 protein240.006
    Actin 2160.026
    Tubulin, β2200.128
Metabolism/transport
    Brain creatine kinase100.039
    Glucose transporter 1 (GLUT-1)70.038
    Glyceraldehyde-3-phosphate dehydrogenase250.109
    Hexokinase 1190.012
    Phosphoglycerate kinase 1150.025
    Sodium/potassium-transporting ATPase340.029
    Transketolase50.004
Vesicular trafficking
    NSF160.008
    Rab 11b150.010
    Rab 1b100.030
    Rab 1890.009
    Rab 3a110.049
    RabGDI (α)3NF
    Syntaxin 380.013
    Syntaxin-binding protein 1 (Munc18-1)220.017
    VAMP2/33NF