Table I

Summary of phosphopeptides identified

PeptidesaPhosphopeptidesaSelectivityPhosphopeptidesSites NRUnique peptidesdUnique sites
ValidatedbNRc
%
HILIC-IMAC8208149910479141004788
IMAC-HILIC89954560684607677438
IMAC147144168109
Totals18781665184913351410
  • a Based on a false positive rate of <1.3%.

  • b Includes peptides manually validated as described in the text.

  • c NR, non-redundant peptides. We eliminated duplicate sequences including alternative charge states and sequence extensions.

  • d Peptides not found in the other two data sets.